Gözde AYDOĞDU TIĞ, Ecem Özlem BOLAT, Bülent ZEYBEK, Şule PEKYARDIMCI

Elektrokimyasal Olarak İndirgenmiş Grafen Oksit ve Poli 2,6- Piridindikarboksilik Asit Modifiye Camsı Karbon Elektrot ile Hesperidin-Dsdna Tayini

Bu çalışmada, camsı karbon elektrot GCE yüzeyi elektrokimyasal olarak indirgenmiş grafen oksit ERGO ile kaplandı ve poli 2,6-piridindikarboksilik asit P PDCA ikinci bir tabaka olarak ERGO modifiye GCE yüzeyinde biriktirildi. Hazırlanan elektrot dsDNA’yı immobilize etmek için kullanıldı. GCE/ERGO/P PDCA elektrodunun elektrokimyasal davranışı dönüşümlü voltametri CV yöntemi ile incelendi ve sonuçlar yalın GCE’den elde edilen değerler ile karşılaştırıldı. Ümit verici bir antikanser ajanı olarak kullanılan hesperidin HDN , GCE/ERGO/P PDCA /dsDNA biyosensörü kullanılarak diferansiyel puls voltametrisi DPV tekniği ile tayin edildi. +0.77 V’daki guanin yükseltgenme pik akımındaki azalma 0.02 M NaCl içeren 0.5 M ABS pH 4.8 ’deki etkileşim için bir indikatör olarak kullanıldı. dsDNA konsantrayonu, HDN konsantrasyonu, dsDNA adsorpsiyon süresi ve etkileşim süresi gibi deneysel parametreler optimize edildi. Guanin yükseltgenme pik akımlarının 0.82-82 µM HDN konsantrasyon aralığında doğrusal olarak değiştiği bulundu ve gözlenebilme sınırı 0.24 µM olarak hesaplandı. Tekrar üretilebilirlik, tekrarlanabilirlik ve analizin insan serum örneklerine uygulanabilirliği incelendi. dsDNA ile HDN arasındaki etkileşim hakkında daha çok bilgi sağlamak amacıyla UVgörünür bölge spektrofotometri ölçümleri de alındı. Hazırlanan yeni dsDNA biyosensörünün hassas, doğru ve hızlı bir şekilde HDN tayini yapabildiği sonucuna varıldı

Anahtar Kelimeler:

Hesperidin, dsDNA, biyosensör, elektrokimya

Hesperidin-dsDNA Interaction Based on Electrochemically Reduced Graphene Oxide and Poly- 2,6-Pyridinedicarboxylic Acid Modified Glassy Carbon Electrode

In this work, electrochemically reduced graphene oxide ERGO was deposited on a glassy carbon electrode GCE and poly 2,6-pyridinedicarboxylic acid P PDCA as a second layer was electrosynthesized on ERGO modified GCE and then, the prepared electrode was used to immobilize dsDNA. Electrochemical behaviour of GCE/ERGO/P PDCA was investigated by using cyclic voltammetry CV and compared with those of the bare GCE. Hesperidin HDN , a promising anticancer agent, can be detected using GCE/ERGO/P PDCA /dsDNA bio- sensor using the differential pulse voltammetry DPV . The decrease in the guanine oxidation peak current at +0.77 V was used as an indicator for the interaction in 0.5 M ABS pH 4.8 containing 0.02 M NaCl. The experi- mental parameters such as dsDNA concentration, HDN concentration, dsDNA adsorption time and interaction time were optimized. The guanine oxidation peak currents were linearly proportional to the concentrations of the HDN in the range of in the range of 0.82-82 μM and detection limit was found to be 0.24 μM. The rep- roducibility, repeatability, and applicability of the analysis to human serum samples were also examined. In order to obtain more information about the interaction between dsDNA and HDN, UV-vis spectrophotometry measurements were also performed. The novel DNA biosensor could serve for sensitive, accurate and rapid determination of the HDN.

Keywords:

Hesperidin, dsDNA, biosensor, electrochemistry,

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