Okratoksin A Tayinine Yönelik Elektrokimyasal Aptasensör Geliştirilmesi

Ç alışmada Okratoksin A’nın OTA işaretsiz bir yöntemle tayinine yönelik elektrokimyasal aptasensör tasarımı gerçekleştirilmiştir. 5’ ucundan tiyohekzil işaretli SH- CH2 6 aptamer dizileri tek kullanımlık perde baskılı altın elektrot AuSCPE yüzeylerine tiyol grubu ile bağlanmıştır. OTA ve DNA aptamer dizisi arasındaki bağlanma Elektrokimyasal Empedans Spektrometri tekniği EIS ile 5mM [Fe CN 6]3-/4- çözeltisi varlığında elektron aktarımına dayalı olarak tayin edilmiş ve tayin sınırı da 0.375 nM olarak saptanmıştır

Anahtar Kelimeler:

Aptasensor, ochratoxin A, electrochemical impedance spectrometry EIS

Development of an Electrochemical Aptasensor for Detection of Ochratoxin A

Asensitive electrochemical aptasensor for label free detection of Ochratoxin A OTA is presented in this study. 5’thiohexyl SH- CH2 6 linked aptamer sequence was immobilized onto disposable screen printed gold electrode surfaces AuSCPE via thiol bounding. Interaction between OTA and DNA aptamer was monitored by Electrochemical Impedance Spectrometry EIS in the presence of 5mM [Fe CN 6]3-/4- based on electron transfer resistance Rct . And a limit of detection was found to be 0.375 nM.

Keywords:

Aptasensor, Ochratoxin A, electrochemical impedance spectrometry EIS,

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