Effect of Surface Functionalization on the Transport Characteristics of Methyl Orange Through Track-Etched Membranes
In this study we have prepared cylindrical and conical nanopores on poly(ethylene terephthalate) (PET) membranes using track-etching method. Later on we have investigated the mass transport of the chosen model dye Methyl Orange (MO) through these membranes. In order to enhance the transport flux of the dye, we have used surface functionalization using ethylenediamine (EDA) as the functionalization agent. We have confirmed the functionalization of the nanopore surface using electrochemical measurements. We have investigated mass transport through functionalized and bare PET membranes and shown that by attaching amine groups on the nanopore walls, we can indeed increase the transport of MO. Effects of pore size, pore geometry and temperature were investigated for the transport of MO. We have shown that PET, which has a negative surface charge at neutral pH, can be functionalized for a more effective transport of negatively charged analyte.
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- 1. Q. Zhai, J. Wang, H. Jiang, Q. Wei, E. Wang, Bare conical
nanopore embedded in polymer membrane for Cr(III)
sensing, Talanta, 140 (2015) 219-225.
- 2. Y.R. Kim, J. Min, I.H. Lee, S. Kim, A.G. Kim, K. Kim, K. Namkoong,
C. Ko, Nanopore sensor for fast label-free detection of short
double-stranded DNAs, Biosens. Bioelectron., 22 (2007)
2926-2931.
- 3. B.M. Venkatesan, A.B. Shah, J.M. Zuo, R. Bashir, DNA Sensing
Using Nanocrystalline Surface-Enhanced Al2O3 Nanopore
Sensors, Adv. Funct. Mater., 20 (2010) 1266-1275.
- 4. L.T. Sexton, P. Jin, K. Kececi, L. Baker, Y. Choi, C.R. Martin,
Resistive pulse sensing of proteins using single conical
nanopores in PET membranes, Abstr. Pap. Am. Chem. S., 231
(2006).
- 5. E.N. Savariar, K. Krishnamoorthy, S. Thayumanavan,
Molecular discrimination inside polymer nanotubules, Nat.
Nanotechnol., 3 (2008) 112-117.
- 6. A.S. Prabhu, T.Z.N. Jubery, K.J. Freedman, R. Mulero, P. Dutta,
M.J. Kim, Chemically modified solid-state nanopores for
high throughput nanoparticle separation, J. Phys. Condens.
Matter., 22 (2010) 454107.
- 7. B. Hornblower, A. Coombs, R.D. Whitaker, A. Kolomeisky,
S.J. Picone, A. Meller, M. Akeson, Single-molecule analysis
of DNA-protein complexes using nanopores, Nat. Meth., 4
(2007) 315.
- 8. C. Dekker, Solid-state nanopores, Nat. Nanotechnol., 2
(2007) 209-215.
- 9. R. Patricio, A. Pavel Yu, C. Javier, M. Salvador, Pore structure
and function of synthetic nanopores with fixed charges: tip
shape and rectification properties, Nanotechnology, 19
(2008) 315707.
- 10. D. Kaya, A. Dinler, N. San, K. Kececi, Effect of Pore Geometry
on Resistive-Pulse Sensing of DNA Using Track-Etched PET
Nanopore Membrane, Electrochim. Acta, 202 (2016) 157-
165.
- 11. B.A. Sartowska, O.L. Orelovitch, A. Presz, P.Y. Apel, I.V.
Blonskaya, Nanopores with controlled profiles in tracketched
membranes, Nukleonika, 57 (2012) 575-579.
- 12. O.A. Saleh, L.L. Saw, Biological sensing with an on-chip
resistive pulse analyzer, P. Ann. Int. IEEE Embs, Vols 1-7, 26
(2004) 2568-2570.
- 13. H.M. Kim, M.H. Lee, K.B. Kim, Theoretical and experimental
study of nanopore drilling by a focused electron beam in
transmission electron microscopy, Nanotechnology, 22
(2011) 275303.
- 14. T. Deng, M. Li, Y. Wang, Z. Liu, Development of solid-state
nanopore fabrication technologies, Chin. Sci. Bull., 60 (2015)
304-319.
- 15. K. Healy, B. Schiedt, A.P. Morrison, Solid-state nanopore
technologies for nanopore-based DNA analysis,
Nanomedicine (Lond), 2 (2007) 875-897.
- 16. D. Kaya, K. Kececi, Preparation of nanopores and their
application for the detection of metals, Bulg. Chem.
Commun., 49 (2017) 37-42.
- 17. S.R. Park, H. Peng, X.S. Ling, Fabrication of nanopores in
silicon chips using feedback chemical etching, Small, 3
(2007) 116-119.
- 18. I. Vlassiouk, P.Y. Apel, S.N. Dmitriev, K. Healy, Z.S. Siwy,
Versatile ultrathin nanoporous silicon nitride membranes,
Proc. Natl. Acad. Sci. USA, 106 (2009) 21039-21044.
- 19. K. Briggs, H. Kwok, V. Tabard-Cossa, Automated fabrication
of 2-nm solid-state nanopores for nucleic acid analysis,
Small, 10 (2014) 2077-2086.
- 20. S. Garaj, W. Hubbard, A. Reina, J. Kong, D. Branton, J.A.
Golovchenko, Graphene as a subnanometer trans-electrode
membrane, Nature, 467 (2010) 190-U173.
- 21. M. Lillo, D. Losic, Ion-beam pore opening of porous anodic
alumina: The formation of single nanopore and nanopore
arrays, Mater. Lett., 63 (2009) 457-460.
- 22. S. Kipke, G. Schmid, Nanoporous alumina membranes as
diffusion controlling systems, Adv. Funct. Mater., 14 (2004)
1184-1188.
- 23. R. Spohr, Status of ion track technology-prospects of single
tracks, Radiat. Meas., 40 (2005) 191-202.
- 24. S. Nasir, M. Ali, W. Ensinger, Thermally controlled
permeation of ionic molecules through synthetic nanopores
functionalized with amine-terminated polymer brushes,
Nanotechnology, 23 (2012) 225502.
- 25. B. Yameen, M. Ali, R. Neumann, W. Ensinger, W. Knoll, O.
Azzaroni, Synthetic Proton-gated ion channels via single
solid-state nanochannels modified with responsive polymer
brushes, Nano Lett., 9 (2009) 2788-2793.
- 26. M. Ali, P. Ramirez, S. Mafé, R. Neumann, W. Ensinger, A pHtunable
nanofluidic diode with a broad range of rectifying
properties, ACS Nano, 3 (2009) 603-608.
- 27. K. Kececi, L.T. Sexton, F. Buyukserin, C.R. Martin, Resistivepulse
detection of short dsDNAs using a chemically
functionalized conical nanopore sensor, Nanomedicine, 3
(2008) 787-796.
- 28. Q.H. Nguyen, M. Ali, R. Neumann, W. Ensinger, Saccharide/
glycoprotein recognition inside synthetic ion channels
modified with boronic acid, Sens. Actuat. B, 162 (2012) 216-
222.
- 29. Z. Siwy, E. Heins, C.C. Harrell, P. Kohli, C.R. Martin, Conicalnanotube
ion-current rectifiers: The role of surface charge,
J. Am. Chem. Soc., 126 (2004) 10850-10851.
- 30. C.C. Harrell, P. Kohli, Z. Siwy, C.R. Martin, DNA - Nanotube
artificial ion channels, J. Am. Chem. Soc., 126 (2004) 15646-
15647.
- 31. K.B. Jirage, J.C. Hulteen, C.R. Martin, Effect of thiol
chemisorption on the transport properties of gold
nanotubule membranes, Anal. Chem., 71 (1999) 4913-4918.
- 32. T.A. Desai, S. Sharma, R.J. Walczak, A. Boiarski, M. Cohen,
J. Shapiro, T. West, K. Melnik, C. Cosentino, P.M. Sinha,
Nanoporous implants for controlled drug delivery, in
BioMEMS and Biomedical Nanotechnology, 2006, Springer.
p. 263-286.
- 33. G. Jeon, S.Y. Yang, J.K. Kim, Functional nanoporous
membranes for drug delivery, J. Mater. Chem., 22 (2012)
14814-14834.
- 34. S.P. Adiga, C. Jin, L.A. Curtiss, N.A. Monteiro‐Riviere,
R.J. Narayan, Nanoporous membranes for medical and
biological applications, Wiley Interdiscip. Rev. Nanomed.
Nanobiotechnol., 1 (2009) 568-581.
- 35. A. Saxena, B.P. Tripathi, M. Kumar, V.K. Shahi, Membranebased
techniques for the separation and purification of
proteins: an overview, Adv. Coll. Interf. Sci., 145 (2009) 1-22.
- 36. K. Kececi, N. San, D. Kaya, Nanopore detection of doublestranded
DNA using a track-etched polycarbonate
membrane, Talanta, 144 (2015) 268-274.
- 37. Z. Siwy, P. Apel, D. Baur, D.D. Dobrev, Y.E. Korchev, R.
Neumann, R. Spohr, C. Trautmann, K.O. Voss, Preparation of
synthetic nanopores with transport properties analogous to
biological channels, Surf. Sci., 532 (2003) 1061-1066.
- 38. Z.S. Siwy, Ion‐Current Rectification in Nanopores and
Nanotubes with Broken Symmetry, Adv. Funct. Mater, 16
(2006) 735-746.
39. J.E. Wharton, P. Jin, L.T. Sexton, L.P. Horne, S.A. Sherrill, W.K.
- Mino, C.R. Martin, A method for reproducibly preparing
synthetic nanopores for resistive-pulse biosensors, Small, 3
(2007) 1424-1430.
- 40. D. Kaya, K. Keçeci, Transport Characteristics of Selected
Dyes Through Track-Etched Multiporous PET Membranes,
Hacettepe J. Biolog. Chem., 46 (2018) 1-11.
- 41. Q.H. Nguyen, M. Ali, S. Nasir, W. Ensinger, Transport
properties of track-etched membranes having variable
effective pore-lengths, Nanotechnology, 26 (2015) 485502.