Türkiye’den Endemik Astragalus argaeus Boiss.’in Antioksidan, Antimikrobiyal ve Sitotoksik Aktivitesi

Bu çalışmada, Türkiye florası için endemik olan Astragalus argaeus’un toprak üstü ve altı kısımlarından elde edilen ekstrelerin toplam fenolik ve flavonoid madde miktarları, in vitro antioksidan, antiradikal, hidrojen peroksit giderme, metal şelatlama, toplam indirgeyici güç, demir indirgeyici toplam antioksidan aktivite (FRAP), bakır (II) iyon indirgeyici antioksidan aktiviteleri araştırılmıştır. Her iki ekstrede de ferulik asit başlıca bileşen olarak tespit edilmiştir. Her iki ekstrede zayıf antioksidan aktivite göstermiştir. Ekstreler agar difüzyon yönteminde 15 mikroorganizmadan sadece Pseudomonas aeruginosa’ya karşı düşük antibakteriyel aktivite göstermişlerdir. Ekstrelerin MCF-7 (insan meme kanser hücre hattı) ve fibroblast hücrelerine karşı sitotoksik etkileri 24 ve 48 saat uygulama sonrası MTT yöntemi ile tespit edilmiştir. Ekstreler 24 saat sonrasında MCF-7 hücrelerine zayıf sitotoksik aktivite göstermişlerdir.

Antioxidant, Antimicrobial and Cytotoxic Activities of Endemic Astragalus argaeus Boiss. from Turkey

In this study, phenolic compound, the total phenolic and flavonoid contents, in vitro antioxidant, antiradical, hydrogenperoxide scavenging, metal chelating, total reducing power, iron ion reducing antioxidant potential (FRAP), copper (II) ionreducing antioxidant capacity (CUPRAC) activities of the extracts obtained from underground and aerial parts of Astragalusargaeus, which is endemic to Turkish flora were investigated. The ferulic acid has been identified as main compound in theboth extracts. Both extracts showed weak antioxidant activities. The extracts showed weak antibacterial activity againstonly Pseudomonas aeruginosa among the 15 microorganisms by agar well diffusion assay. The cytotoxic effects of extractson MCF-7 (human breast cancer cell lines) and fibroblast cells during 24 and 48 hours were determined by MTT method. Theextracts showed weak cytotoxic activity on MCF-7 after 24h.

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