Electroanalytical Studies and Simultaneous Validated Assay of Antihypertensive Compounds in Their Binary Mixtures Using Fast Electrochemical Technique
The electrochemical behavior of amlodipine (AML) and telmisartan (TLM) on a glassy carbon electrode were investigated in different aqueous solutions by using cyclic, differential pulse, and square wave voltammetric techniques. AML and TLM exhibited one peak (both of them) to the anodic direction. The oxidation process was found to be irreversible and adsorption controlled. To obtain good sensitivity, the instrumental and accumulation variables were studied using square wave voltammetry (SWV) techniques in 0.5 M H2SO4 solution, which was obtained as the most repeatabile and sensitive media. In this study; sensitive, fast and reliable method development was aimed for simultaneous detection of AML and TLM. This voltammetric method exhibited linear dynamic responses in the concentration range between 1.0×10-7 M and 1.0×10-4 M for AML and 1.0×10-7 M and 1.0×10-5 M for TLM, with detection limits of 0.654 nM and 22.6 nM, respectively. The proposed method is validated and successfully applied for the analysis of AML and TLM tablets.
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- R.A. Burges, D.G. Gardiner, M. Gwilt, A.J. Higgins, K.J.
Blackburn, S.F. Campbell, P.E. Cross, J.K. Stubbs, Calcium
channel blocking properties of amlodipine in vascular
smooth muscle and cardiac muscle in vitro: evidence for
voltage modulation of vascular dihydropyridine receptors, J.
Cardiovas. Pharm., 9 (1987) 110-9.
- 2. A.A.K. Gazy, Determination of amlodipine besylate by
adsorptive square-wave anodic stripping voltammetry
on glassy carbon electrode in tablets and biological fluids,
Talanta, 62 (2004) 575-582.
- 3. T. Manuel, V. Velasquez, Angiotensin II receptor blockers:
a new class of antihypertensive drugs, Arch. Fam. Med., 5
(1996) 351-356.
- 4. H. Yi, W.H.-C. and S.B. Biointerfaces, undefined 2007,
Enhancement effect of sodium dodecyl benzene sulfonate
(SDBS) and its application into voltammetric determination
of telmisartan, Elsevier. (n.d.).
- 5. M.S. Kondawar, K.G. Kamble, K.S. Raut, K.H. Maharshi, UV
Spectrophotometric estimation of Amlodipine besylate
and Telmisartan in Bulk drug and Dosage form by
Multiwavelength Analysis, Intern. J. Chem. Tech Res. 3 (n.d.)
974-4290.
- 6. A.S. Arul, G. Dhas, A.K. Muthu, R. Sankhla, S. Gupta, A.A.
Smith, R. Manavalan, Development and validation of a
reversed phase hplc method for simultaneous determination
of amlodipine and telmisartan in pharmaceutical dosage
form, article in journal of chemical research, J. Appl. Chem.
Res., 12 (2010) 43-52.
- 7. A.R. Chabukswar, S.C. Jagdale, S. V Kumbhar, V.J. Kadam, V.D.
Patil, B.S. Kuchekar, P.D. Lokhande, Simultaneous HPTLC
estimation of telmisartan and amlodipine besylate in tablet
dosage form, Arch. Appl. Sci. Res., 2 (2010) 94-100.
- 8. S. Nalwade, V.R. Reddy, D.D. Rao, I.K. Rao, Rapid
Simultaneous determination of telmisartan, amlodipine
besylate and hydrochlorothiazide in a combined poly pill
dosage form by stability-indicating ultra performance liquid
chromatography, Scientia Pharmaceutica. 79 (2011) 69-84.
- 9. R.N. Goyal, N. Bachheti, A. Tyagi, A.K. Pandey, Differential
pulse voltammetric determination of methylprednisolone
in pharmaceuticals and human biological fluids, Anal. Chim.
Acta, 12 (2007) 34-40.
- 10. N. Karadas-Bakirhan, M. Gumustas, B. Uslu, S.A. Ozkan,
Simultaneous determination of amlodipine besylate and
rosuvastatin calcium in binary mixtures by voltammetric
and chromatographic techniques, Ionics, 22 (2016) 277-288.
- 11. B.S. Goyal, Voltammetric determination of amlodipine
besylate in human urine and pharmaceuticals,
Bioelectrochem., 79 (2010) 234-240.
- 12. B. Bozal-Palabiyik, B. Dogan-Topal, B. Uslu, A. Can, S.A.
Ozkan, Sensitive voltammetric assay of etoposide using
modified glassy carbon electrode with a dispersion of multiwalled
carbon nanotube, J. Solid State Electrochem., 17
(2013) 2815-2822.
- 13. S. Kurbanoglu, B. Dogan-Topal, B. Uslu, A. Can, S.A.
Ozkan, Electrochemical investigations of the anticancer
drug idarubicin using multiwalled carbon nanotubes
modified glassy carbon and pyrolytic graphite electrodes,
Electroanalysis, 25 (2013) 1473-1482.
- 14. C. Brett, A. Brett, Electrochemistry: Principles, Methods,
and Applications, Springer, (1993) 427.
- 15. F. Agin, N. Karadas, B. Uslu, S.A. Ozkan, Electroanalytical
characterization of levodropropizine and its voltammetric
determination in pharmaceuticals, Curr. Pharm. Anal., 9
(2013).
- 16. S.A. Ozkan, J.M. Kauffmann, P. Zuman, Electroanalysis in
Biomedical and Pharmaceutical Sciences, Springer Berlin
Heidelberg, Berlin, Heidelberg, 2015.
- 17. N. Karadas, S. Sanli, M. Gumustas, S.A. Ozkan, Voltammetric
and RP-LC assay for determination of benidipine HCl, J.
Pharm. Biomed. Anal., 66 (2012).
- 18. N. Karadas, S. Sanli, B. Akmese, B. Dogan-Topal, A. Can,
S.A. Ozkan, Analytical application of polymethylene bluemultiwalled
carbon nanotubes modified glassy carbon
electrode on anticancer drug irinotecan and determination
of its ionization constant value, Talanta, 115 (2013) 911-919.
- 19. N. Karadas, S.A. Ozkan, Electrochemical preparation of
sodium dodecyl sulfate doped over-oxidized polypyrrole/
multi-walled carbon nanotube composite on glassy carbon
electrode and its application on sensitive and selective
determination of anticancer drug: Pemetrexed, Talanta, 119
(2014).
- 20. S.A. Ozkan, Electroanalytical Methods in Pharmaceutical
Analysis and Their Validation, First edit, HNB Pub., 2012.
- 21. M. Gumustas, N. Karadas, S.A. Ozkan, Validated
electroanalytical and RP-LC assay of ertapenem in its
pharmaceutical dosage form, Rev. Roum. Chim., 58 (2013)
679-690