Pitaya (hylocereus guatemalensis) kabuğu ekstraktlarının bazı metabolik enzimler üzerine olan inhibisyon etkisinin araştırılması

Pitaya, çeşitli kullanımları olan tropikal ve subtropikal bir bitkidir. Çalışmada maserasyon yöntemi ile pitaya meyvesinin kabuk kısımlarından aseton, etanol, etil asetat, diklorometan ve metanol ekstraktları elde edilmiştir. Meyve kabuğu ekstraktlarının kritik metabolik enzimler olan asetilkolinesteraz (AChE), butirilkolinesteraz (BChE), insan karbonik anhidraz CA I- II ve α-glikozidaz enzim aktiviteleri üzerine in vitro etkileri araştırıldı. AChE için Pitaya kabuğu etanol (PBE) (IC50: 20.16±0.22 µg/mL, r2:0.9694) ve BChE için Pitaya kabuğu aseton (PBA) (IC50:42.10±1.045 µg/mL, r2:0.9807) ekstraktı en güçlü inhibisyon etkisini gösterdi. Pitaya kabuğu etil asetat (PBEA) ekstresi hCA-I ve II için en kuvvetli inhibitör etkiyi gösterdi (sırasıyla IC50:53.51±2.22, r2:0.9562 µg/mL, IC50:26.29±0.34 µg/mL, r2:0.9966). Ek olarak PBEA, a-Glikozidaz enzimi üzerinde en güçlü inhibisyon etkisini göstermiştir (IC50:24.54±1.80 µg/mL, r2:0.9199). Sonuçlara göre ekstraktlar glokom, epilepsi tip 2 Diabetes mellitus (T2DM) ve Alzheimer hastalığı (AH) gibi hastalıkların tedavisinde etkin bir şekilde kullanılabilir.

Anahtar Kelimeler:

Enzim, Enzim İnhibisyonu, Pitaya

Investigation of the inhibition effect of pitaya (hylocereus guatemalensis) bark extracts on some metabolic enzymes

Pitaya is a tropical and subtropical plant with various uses. In this study, the maceration method obtained acetone, ethanol, ethyl acetate, dichloromethane, and methanol from the bark parts of pitaya fruit. The in vitro effects of fruit peel extracts on the critical metabolic enzyme activities of acetylcholinesterase (AChE), butyrylcholinesterase (BChE), human carbonic anhydrase (hCA I and hCA II), and α-glycosidase, were investigated. Pitaya bark ethanol (PBE) (IC50: 20.16±0.22 µg/mL, r2:0.9694) for AChE and Pitaya bark acetone (PBA) (IC50:42.10±1.045 µg/mL, r2:0.9807) extract for BChE showed the most potent inhibition effect. Pitaya bark ethyl acetate (PBEA) extract showed the most potent inhibitory effect for hCA I and hCA II (IC50:53.51±2.22 µg/mL, r2:0.9562 and IC50:26.29±0.34 µg/mL, r2:0.9966), respectively. In addition, PBEA showed the most potent inhibition effect on α-glycosidase enzyme (IC50: 24.54±1.80 µg/mL, r2:0.9199). According to the results, the extracts can be effectively used treatment of diseases such as glaucoma, epilepsy, type 2 Diabetes mellitus (T2DM), and Alzheimer's disease (AD).

Keywords:

Enzyme, Enzyme Inhibition, Pitaya,

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