Cihan BOSTANCI, Sevgi ERTUĞRUL KARATAY, Gönül DÖNMEZ

YER FISTIĞI KABUĞUNDAN BİYOETANOL ÜRETİMİ

İkinci nesil biyoyakıtların önemi her geçen gün artmaktadır. Yer fıstığı ülkemizde bol miktarda yetişen bir tarım ürünüdür. Lignoselülozik bir tarımsal atık olan yer fıstığı kabuğu ikinci nesil biyoyakıt üretiminde biyokütle olarak kullanılabilmektedir. Yapılan deneylerde, üretilen biyoetanolü optimize etmek amacıyla farklı kabuk konsantrasyonları, hidroliz koşulları, Accellerase 1500 enzim kullanımı, inkübasyon koşulları, inokülasyon oranı ve farklı azot kaynakları denenmiştir. Denemeler sonucunda, enzimatik hidrolizle %10 kabuk ve 1 g/L soya unu ile hazırlanan besiyerlerinde, 12 saatte, aerobik koşullarda ve maya optik yoğunluğu 0.86 olduğunda etanol üretiminin 2.24 g/L olduğu belirlenmiştir. İnokülasyon oranı ve inkübasyon süresi arttığında maya gelişimi OD600: 6.0’a kadar yükselmiş ancak etanol üretimi düşmüştür.

Anahtar Kelimeler:

Yer fıstığı kabuğu, biyoetanol, yenilenebilir enerji, biyokütle

BIOETHANOL PRODUCTION FROM PEANUT SHELL

The importance of second generation biofuels is increasing day by day. Peanut is an agricultural product that is abundantly produced in Turkey. Peanut shell which is a lignocellulosic agricultural biomass may be used to produce biofuel. In experiments to optimise bioethanol production, different shell loading concentrations, pretreatment conditions, the usage of Accellerase 1500 enzyme, incubation conditions, inoculation ratios and different nitrogen source were studied. In the media preparing with peanut shell sugar, obtaining after enzymatic hydrolysis, the bioethanol concentration and yeast growth was obtained as 2.24 g/L and 0.86 (OD600) in the presence of 10% shell loading and 1 g/L soy flour at the end of 12 hours incubation time in aerobic conditions. Higher yeast growth such as 6.0 (OD600), lower bioethanol production were obtained when the inoculation ratio and incubation times were increased.

Keywords:

Peanut shell, bioethanol, renewable energy, biomass,

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