UÇUCU YAĞ İÇEREN KİTOSAN BAZLI FİLMLERİN KARAKTERİZASYONU

Bu çalışmada, kekik yağı (TO, %2), üzüm çekirdeği yağı (GSO, %2) ve nar çekirdeği yağı (PSO, %2) eklenmiş kitosan (CH) filmler hazırlanmıştır. Film örnekleri, taramalı elektron mikroskobu (SEM) ile elde edilen görüntüler, su buharı geçirgenliği (WVP), mekanik testler ve optik özellikler ile karakterize edilmiştir. Antimikrobiyel aktivite ve filmlerin salım davranışı da incelenmiştir. SEM görüntüleri, CH-TO filmlerin, CH-GSO ve CH-PSO filmlerle karşılaştırıldığında daha homojen bir yapıya sahip olduğunu göstermektedir. CH filmlerin elastikiyeti yağ ilavesiyle geliştirilirken, filmlerin gerilme kuvvetinde önemli bir değişiklik gözlenmemiştir. CH filmlere esansiyel yağ ilavesi, filmlerin kalınlık, WVP ve su tutma kapasitesi değerlerinde önemli ölçüde azalışa neden olurken (p<0.05), filmlerin çözünürlük değerleri etkilenmemiştir. CH filmlere esansiyel yağ ilavesi, filmlerin optik özelliklerini etkilememiştir (p>0.05). En yüksek salım hızı, CH-TO film örneklerinde gözlenmiştir (p<0.05). Tüm film örnekleri, Escherichia coli, Staphylococcus aureus, Listeria monocytogenes ve Pseudomonas aeruginosa’ya karşı antimikrobiyel aktivite göstermiştir.

Anahtar Kelimeler:

Kitosan, kekik yağı, üzüm çekirdeği yağı, nar çekirdeği yağı, antimikrobiyel

CHARACTERIZATION OF CHITOSAN BASED FILMS INCORPORATED WITH ESSENTIAL OILS

In this study, thyme oil (TO, 2%), grape seed oil (GSO, 2%), and pomegranate seed oil (PSO, 2%) added chitosan (CH) films were prepared. Film samples were characterized by scanning electron microscopy (SEM), water vapor permeability (WVP), tensile, and optical properties. The antimicrobial activity and release behavior of films were also studied. SEM images showed that CH-TO films were more homogeneous when compared to CH-GSO, and CH-PSO films. The elasticity of films was improved upon the addition of oils while there was no significant change in tensile strength. The incorporation of oils significantly reduced thickness, WVP and water-uptake values of films (p<0.05) while the solubility did not change significantly. The optical properties of films were not significantly affected by the addition of oils. The highest release rate was observed in CH-TO film samples (p<0.05). All film samples showed antimicrobial activity against Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Pseudomonas aeruginosa.

Keywords:

Chitosan, thyme oil, grape seed oil, pomegranate seed oil, antimicrobial,

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