Çise KARAMAN, Seher KUMCUOĞLU, Sibel KAYA BAYRAM, Şebnem TAVMAN

TAVUK STERNUMUNDAN ENZİMATİK HİDROLİZASYON İLE GLİKOZAMİNOGLİKAN (GAG) ÜRETİMİ VE İŞLEM PARAMETRELERİNİN OPTİMİZASYONU

Bu çalışmada tavuk sternumundan enzimatik hidrolizasyon yöntemiyle sülfatlanmış glikozaminoglikan (GAG) ekstraksiyonu gerçekleştirilmiş ve optimum enzimatik hidrolizasyon işlem koşulu belirlenmiştir. İşlem koşullarının optimizasyonu için Yanıt Yüzey Yöntemi kullanılmıştır. Enzim miktarı, pH değeri, ekstraksiyon sıcaklığı ve süresi olarak seçilen dört parametrenin GAG verimi üzerindeki etkilerini açıklamak için Merkezi Kompozit Tasarımıyla oluşturulan deneme deseni uygulanmıştır. En yüksek GAG verimine en kısa ekstraksiyon süresi ve en az enzim miktarı ile ulaşmak hedeflenmiştir. Ekstraksiyon işlemi için en uygun işlem koşulları %0.6 enzim miktarı, 6.99 pH değeri, 62.96°C ekstraksiyon sıcaklığı ve 10.79 saat ekstraksiyon süresi olarak bulunmuştur. Bu koşullarda, ekstraksiyon verim değerinin %26.07 olması öngörülmüş ve deneysel olarak da doğrulanması sağlanmıştır.

Anahtar Kelimeler:

Tavuk sternumu, glikozaminoglikan, GAG, enzimatik ekstraksiyon, optimizasyon

PRODUCTION OF GLYCOSAMINOGLYCAN (GAG) FROM CHICKEN STERNUM BY ENZYMATIC HYDROLYSIS AND OPTIMIZATION OF PROCESS PARAMETERS

In this study, sulfated glycosaminoglycan (GAG) extraction was performed from chicken sternum by enzymatic hydrolysis method and optimum enzymatic hydrolysis process conditions were determined. Response Surface Method was used for the optimization of the process conditions. In order to determine the effects of the selected four parameters as enzyme amount, pH value, extraction temperature and time on GAG yield, a trial design created with Central Composite Design was applied. It is aimed to reach the highest GAG efficiency in the shortest time and by using the least amount of enzyme. The most suitable process conditions for the extraction process were found to be 0.6% enzyme amount, 6.99 pH value, 62.96°C extraction temperature and 10.79 hours extraction time. Under these conditions, the extraction yield value was predicted to be 26.07% and it was confirmed experimentally.

Keywords:

Chicken sternum, glycosaminoglycan, GAG, enzymatic extraction, optimization,

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