Polisilik aromatik hidrokarbon (PAH)' lardan benzo (A) pirenin sızma, riviera ve prina zeytinyağlarında belirlenmesi

Bu çalışma, 20 adet naturel sızma zeytinyağı, 20 adet riviera zeytinyağı ve 10 adet rafine prina yağında bulunan benzo(a)piren (BaP) miktarlarını belirlemek amacıyla yapılmıştır. Örneklerin hazırlanmasında silika kartuş kullanılmış ve katı faz ekstraksiyonu yapılmıştır. Analizler fluoresans dedektörü ile kombine edilmiş HPLC cihazında yapılmıştır. Yöntemde ortalama geri alma oranı % 76,08 ± 1,49, tespit edilebilir en düşük miktar 0,3 µg/kg olarak belirlenmiştir. Bu bilgiler, yöntemin zeytinyağlarındaki BaP miktarının belirlenmesinde rutin olarak kullanılabileceğini göstermiştir. Analizi yapılan naturel sızma zeytinyağı örneklerinin 3'ünde 0,330 µg/kg ile 0,870 µg/kg arasında BaP tespit edilmiştir. Bu örnekler için ortalama BaP miktarı 0,539 ± 0,167µg/kg'dır. Riviera zeytinyağının 8'inde tespit edilebilir düzeyde BaP kalıntısına rastlanmazken, 12'sinde 2,465 µg/kg seviyesine kadar BaP bulunmuştur. BaP tespit edilen örneklerde ortalama miktar 0,737 ± 0,192 µg/kg olarak saptanmıştır. Prina yağlarının çoğunda yüksek miktarlarda BaP bulunmuştur. BaP tespit edilen 7 örnekte ortalama miktar 31,472 ± 7,42 µg/kg (en az: 0,460 µg/kg, en çok: 58,241 µg/kg)'dır. Naturel sızma zeytinyağı, riviera zeytinyağı ve prina yağlarının ortalamaları arasındaki farklılıkların irdelenmesinde varyans analizi tekniği kullanılmış ve ortalamalar arasındaki farkların önemli olduğu saptanmıştır. Farklı yağ gruplarının tespitinde ise DUNCAN testinden yararlanılmış, sadece prina yağı ortalaması ile diğer yağ örneklerinin ortalamaları arasındaki farkların istatistik olarak önemli olduğu belirlenmiştir (p< 0,01).

Determination of benzo (A)pyrene from polycyclic aromatic hydrocarbons in extra virgin, riviera and olive pomace oils

This study was carried out to determine the amount of benzo(a)pyrene in 20 of extra virgin olive oils, 20 of riviera olive oils and 10 of refined olive pomace oils. Solid phase extraction (silica cartidge) was used in the preparation of samples. Analyses were made by HPLC combined with fluorescence detector. In this method, mean of recovery was found % 76.08 ± 1.49 and limit of detection was 0.3 µg/kg. The findings showed that this method can be used for determining benzo(a)pyrene in olive oils for routine analysis. In three of the extra virgin olive oil samples analysed, BaP was determined between 0.330 µg/kg and 0.870 µg/kg and mean concentration was 0.539 ± 0.167µg/kg. BaP was not determined at limit of dedection in eight of riviera olive oils samples whereas; it was found 2.465 µg/kg in 12 of these samples. In the samples containing BaP, mean concentration of BaP was determined 0.737 ± 0.192 µg/kg. In the most of the olive pomace oils, BaP was found at the highest level. The mean concentration of BaP was 31.472 ± 7.42 µg/kg (min: 0.460 µg/kg, max: 58.241 µg/kg) in 7 samples containing BaP. Results were analyzed by the one-way analysis of variance for determining the differences among the BaP concentrations of extra virgin olive oils, riviera olive oils, olive pomace oils. It was found that the mean concentrations were significantly different (p≤0.09) DUNCAN test for comparision among means, only mean concentration of BaP in olive pomace oil was statistically significant (p< 0.01).

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