Naciye KUTLU KANTAR, Cansu BIÇAK, Duygu EKİNCİ, Ezgi KILIÇ, Naz ERDEM, Aslı İŞCİ YAKAN, Özge ŞAKIYAN DEMİRKOL

LİKAPADAN (Vaccinium arctostaphylos) TOPLAM FENOLİK BİLEŞİKLERİN MİKRODALGA DESTEKLİ EKSTRAKSİYONUNUN YANIT YÜZEY YÖNTEMİ İLE OPTİMİZASYONU

Mikrodalga-destekli ektraksiyon (MAE), konvansiyonel tekniklerle ile karşılaştırıldığında ekstraksiyon süresi ile solvent tüketimini düşürürken, ekstrakt kalitesini arttırması nedeniyle yenilikçi bir yöntem olarak son yıllarda yaygın bir şekilde kullanılmaktadır. Bu çalışmada, mikrodalga gücü, katı konsantrasyonu ve ekstraksiyon süresinin, likapa meyvesinin toplam fenolik içeriği (TPC), renk değerleri ve dielektrik özellikleri üzerine etkisi incelenmiştir. Proses değişkenleri yanıt yüzey yöntemi kullanılarak optimize edilmiştir. Likapadan en yüksek TPC, 360 W mikrodalga gücü, %15 katı konsantrasyonu ve 3.5 dakika ekstraksiyon süresi koşullarında elde edilmiştir. Sonuçlara bakıldığında, ekstrakların L* ve a* değerleri, TPC ile ikinci dereceden polinom ile yüksek korelasyon göstermiştir. Ayrıca, grupların dielektrik özellikleri arasında anlamlı fark bulunamamıştır.

Anahtar Kelimeler:

Likapa, mikrodalga-destekli ekstraksiyon, optimizasyon, toplam fenolik içerik, dielektrik

APPLICATION OF RESPONSE SURFACE METHODOLOGY TO OPTIMIZE MICROWAVE-ASSISTED EXTRACTION OF TOTAL PHENOLIC COMPOUNDS FROM CAUCASIAN WHORTLEBERRY

Microwave-assisted extraction (MAE) is commonly used in recent years as an innovative approach to enhancing the quality of extracts while decreasing the extraction time and the solvent consumption in comparison to conventional techniques. In this study, the influence of microwave power, solid concentration and extraction time on total phenolic content (TPC), colour values and dielectric properties of Caucasian whortleberry were investigated. The process variables were optimized using response surface methodology. The highest TPC from Caucasian whortleberry was obtained at an extraction time of 3.5 min, a solid concentration of 15% and a microwave power of 360 W. The results showed that L* and a* values of the extracts were highly correlated with TPC by a second-order polynomial. Moreover, no significant difference was found in dielectric properties between the groups.

Keywords:

Caucasian whortleberry, microwave-assisted extraction, optimization, total phenolic content, dielectric,

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