KIRMIZI TURP (Raphanus sativus L.) İÇ VE KABUKLARINDAN POLİFENOLİKLERİN ULTRASES SU BANYOSU VE ULTRASES PROB DESTEKLİ EKSTRAKSİYONU: IN VITRO SİNDİRİM SIRASINDA ANTİOKSİDAN AKTİVİTEDEKİ DEĞİŞİMİN ARAŞTIRILMASI

Bu çalışmada, kırmızı turp (Raphanus sativus L.) kabuk ve içlerinden elde edilen polifenolik ekstraktların in vitro sindirim sırasında toplam fenolik madde miktarı (TFMM) ve antioksidan aktivitesindeki (AOA) değişim araştırılmıştır. Öncelikle, utrases su banyosu destekli ekstraksiyon (USDE) ve ultrases prob destekli ekstraksiyon (UPDE), farklı ultrasonikasyon süreleri (20, 60, 180, 540 saniye) ile uygulanarak en yüksek TFMM ve AOA sağlayan ekstraksiyon yöntemi belirlenmiştir. Örneklerin TFMM içeriği Folin-Ciocalteu metodu ile AOA’si ise DPPH ve CUPRAC metotları ile belirlenmiştir. Sonuçlara göre, en yüksek TFMM ve AOACUPRAC değerleri, turp içleri için sırasıyla 41.17±0.70 mg GAE/g kuru madde (KM) ve 12.67±0.00 mg TE/g KM, turp kabukları için sırasıyla 243.26±5.44 mg GAE/g KM ve 89.65±0.48 mg TE/g KM olarak 60 saniye ultrasonikasyon süresinde USDE ile elde edilmiştir. Turp iç ve kabuklarından ekstraktların in vitro sindirimden sonra biyoerişilebilirliği (%) sırasıyla, TFMM için %54.21±1.94 ve %32.20±2.61, AOACUPRAC için %66.46±0.40 ve %27.89±0.99 ve AOADPPH için %302.03±28.52 ve %80.43±1.03 olarak gerçekleşmiştir.

Anahtar Kelimeler:

Kırmızı turp, polifenolikler, antioksidan aktivite, in vitro sindirim.

ULTRASOUND BATH- AND ULTRASOUND PROBE-ASSISTED EXTRACTIONS OF POLYPHENOLICS FROM PULP AND PEEL OF RED RADISH (Raphanus sativus L.): INVESTIGATION OF CHANGES IN ANTIOXIDANT ACTIVITY DURING IN VITRO DIGESTION

In this study, changes in total phenolic content (TPC) and antioxidant activity (AOA) of polyphenolics from pulp and peel of red radish (Raphanus sativus L.) were investigated during in vitro digestion. Firstly, ultrasound bath-assisted extraction (UBAE) and ultrasound probe-assisted extraction (UPAE) were applied with different ultrasonication time (20, 60, 180, 540 seconds) to determine the extraction method providing the highest TPC and AOA. The TPC and AOA (two methods) were determined by the Folin-Ciocalteu, DPPH and CUPRAC methods, respectively. According to the results, the highest TPC and AOACUPRAC were 41.17±0.70 mg GAE/g dry weight (DW) and 12.67±0.00 mg TE/g DW for pulp and 243.26±5.44 mg GAE/g DW and 89.65±0.48 mg TE/g DW for peel extracts in UBAE, respectively. The bioaccessibilities (%) of extracts from pulp and peel after in vitro digestion process were %54.21±1.94 and %32.20±2.61 for TPC, %66.46±0.40 and %27.89±0.99 for AOACUPRAC and %302.03±28.52 and %80.43±1.03 for AOADPPH, respectively.

Keywords:

Red radish, polyphenolics, antioxidant activity, in vitro digestion.,

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