GEÇ ŞİŞME ETMENİ CLOSTRİDİAL TÜRLERİN SPORULASYON SÜRESİNCE ENDOSPOR YAPISAL VE UÇUCU BİLEŞİKLERİNİN BELİRLENMESİ

u çalışmada, çeşitli örneklerden izole edilen ve peynirde geç şişme kusuruna neden olan clostridial türlerin sporulasyon süresince endospor yapısal ve uçucu bileşikleri araştırılmıştır. Gram boyama, katalaz ve oksidaz testi, anaerobik gelişim testleri ile morfolojik olarak tanımlanan türler 16S rRNA gen sekans analizi ile doğrulanmıştır. Sporulasyon süresince, yüksek gaz üretim kapasitesine sahip suşlar Taramalı Elektron Mikroskobu (SEM) ve gaz kromatografisi-kütle spektrometresi (GC-MS) ile analiz edilmiştir. Sporulasyon sonunda bütanol, pentanol, ethanol, hexanol, proponol, oktanol gibi alkol bileşikleri; sülfit, ester ve asit grubu bileşikler öne çıkan bileşenlerdendir. Toplam bağıl yüzde alanı >%50 sülfit grubu bileşiğe sahip olan Clostridium sporogenes suşlarının hücre duvarı kalıntıları ve endospor yapısal bileşenleri; bütanol üretim düzeyi yüksek olan Clostridium butyricum suşlarına göre daha yoğun şekilde gözlemlenmiştir. Bu çalışmadan elde edilen bilgiler geç şişme etmeni clostridial türlerin endospor yapısal ve uçucu bileşiklerinin tespitine yönelik çalışmalara kolaylık sağlayacaktır.

Anahtar Kelimeler:

Clostridium spp., GC-MS, SEM, geç şişme, 16S rRNA

DETERMINATION OF ENDOSPORE STRUCTURE AND VOLATILE COMPOUNDS OF CLOSTRIDIAL SPECIES DURING SPORULATION

In this study, endospore structural and volatile compounds of clostridial species causing late blowing defect (LBD) in cheese were investigated during sporulation. The species were morphologically identified by Gram staining, catalase and oxidase tests, and anaerobic growth tests, and were confirmed by 16S rRNA gene sequence analysis. Strains with high gas production capacity were analyzed by Scanning Electron Microscopy (SEM) and gas chromatography-mass spectrometry (GC-MS) during sporulation. At the end of sporulation, alcohols such as butanol, pentanol, ethanol, hexanol, propanol, octanol, as well as sulfite, ester and acid group compounds were the prominent components. The cell wall residues and endospore structural components of Clostridium sporogenes strains with a total relative percentage area of >50% sulfite group compound were observed more intensively than Clostridium butyricum strains with high butanol production levels. The information obtained from this study will facilitate studies aimed at identifying endospore structural and volatile compounds of LBD clostridial species.

Keywords:

Clostridium spp., GC-MS, SEM, late blowing, 16S rRNA,

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