Vasfi Mehmet BALKİ, Nükhet Nilüfer DEMİREL ZORBA, Nesrin ÇAKICI

YEŞİL SEBZELERDE GENİŞLEMİŞ SPEKTRUMLU BETA LAKTAMAZ VE KARBAPENEMAZ ÜRETEN ENTEROBACTERALES SUŞLARININ ARAŞTIRILMASI

Çanakkale semt pazarlarında satılan 96 adet yeşil sebze örneğinde “beta laktam” dirençli Enterobacterales suşlarının varlığı araştırılmıştır. Gövde, yaprak kısımlarından alınan örneklerden Violet Red Bile Glucose (VRBG) Agara ekilmiştir. Enterobacterales yükleri <1 log KOB/g ile 6.11 log KOB/g arasında tespit edilmiştir. Kromojenik GSBL Agar ve 2 mg/ml meropenem içeren EMB Agarda üreyen 129 adet izolat MALDI-TOF MS yöntemine göre Serratia liquefaciens (n=35), Serratia plymuthica (n=31), Klebsiella oxytoca (n=25), Klebsiella pneumonia (n=6), Raoultella ornithinolytica (n=14), Raoultella terrigena (n=3), Enterobacter cloacae (n=11), Enterobacter ludwigii (n=4), Enterobacter asburiae (n=1), Lelliottia amnigena (n=16), Escherichia coli (n=2), Escherichia hermannii (n=1), Leclercia adecarboxylata (n=3), Pantoea agglomerans (n=3), Kluyvera intermedia (n=2), Kosakonia cowanii (n=1) ve Hafnia alvei (n=1) olarak tanımlanmıştır. Disk difüzyon yöntemine göre izolatların %11.62’sinin (n=15) Genişlemiş Spektrumlu “Beta Laktamaz” (GSBL), %4.65’inin (n=6) karbapenemaz pozitif olduğu belirlenmiştir. Yeşil sebzelerde GSBL ve karbapenem dirençli izolatların varlığı ısıl işlem görmeden tüketilen bu gıdalardaki halk sağlığı riskini ortaya koymaktadır.

Anahtar Kelimeler:

Enterobacterales, GSBL, Karbapenemaz, Antimikrobiyal direnç

INVESTIGATION OF EXTENDED SPECTRUM BETA-LACTAMASE AND CARBAPENEMASE PRODUCING ENTEROBACTERALES STRAINS IN GREEN VEGETABLES

The presence of beta-lactam resistant Enterobacterales strains was investigated in 96 green vegetable samples sold in Çanakkale neighborhood markets. Samples taken from stem and leaf parts were inoculated in Violet Red Bile Glucose (VRBG) Agar. Enterobacterales loads were determined between <1 log CFU/g and 6.11 log CFU/g. One hundred twenty-nine isolates grown on EMB Agar containing 2 mg/ml meropenem and chromogenic ESBL Agar were identified as, Serratia liquefaciens (n=35), Serratia plymuthica (n=31), Klebsiella oxytoca (n=25), Klebsiella pneumonia (n=6), Raoultella ornithinolytica (n=14), Raoultella terrigena (n=3), Enterobacter cloacae (n=11), Enterobacter ludwigii (n=4), Enterobacter asburiae (n=1), Lelliottia amnigena (n=16), Escherichia coli (n=2), Escherichia hermannii (n=1), Leclercia adecarboxylata (n=3), Pantoea agglomerans (n=3), Kluyvera intermedia (n=2), Kosakonia cowanii (n=1) and Hafnia alvei (n=1) with MALDI-TOF MS method. According to the disk diffusion method, 11.62% (n=15) of the isolates were found to be Extended Spectrum Beta Lactamase (ESBL) and 4.65% (n=6) carbapenemase positive. ESBL and carbapenem-resistant isolates in green vegetables reveal the public health risk in these foods consumed without heat treatment.

Keywords:

Enterobacterales, ESBL, Carbapenemase, Antimicrobial resistance,

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