Anabaena sp.'nin pigment içerikler üzerine glukozun etkisi

Gıda için doğal renklendiriciler, yenilenebilir kaynaklardan yapılmaktadırlar. Renklendiriciler genellikle mikroorganizmalardan ekstrakte edilmektedirler, ancak bitki materyali, böcek, algler, siyanobakteriler ve funguslar gibi diğer kaynaklar da kullanılmaktadır. Karbon ve azot siyanobakterilerde pigmentleri etkileyen en önemli çevresel faktörlerdendir. Glukoz, enerji ve metabolizma düzenleyici bir kaynak olmasının yanı sıra, hücrelerde ki klorofil-a, fikosiyanin, allofikosiyanin ve E-karoten gibi pigment içeriklerini de farklı olarak etkilemektedir. Mevcut araştırmada farklı glukoz konsantrasyonlarının (10- 320 mM) Anabaena sp.'nin klorofil-a, fikosiyanin, allofikosiyanin ve E-karoten içerikleri üzerine etkilerini araştırmayı amaçlamıştır. Maksimum pigment içerikleri 10 mM glukoz konsantrasyonunda saptanmıştır. Ancak, 40 mMdan yüksek glukoz konsantrasyonlarında, bu türün pigment içerikleri ciddi bir şekilde azaltılmıştır. Anabaena sp. GO10, 60 mM ve daha yüksek glukoz konsantrasyonları tarafından tamamen baskılanmıştır. Bu araştırmada, glukozun, pigment üretimi için karbon kaynağı olarak kullanıldığı gösterilmiştir. Günümüze kadar yapılan çalışmalarda, siyanobakterilerin pigmentleri üzerine glukozun etkisine yönelik çok az sayıda bilgi bulunmaktadır.

The effect of glucose on pigment contents of anabaena sp.

Natural colorants for food are made from renewable sources. Most often, the colorants are extracted from microorganisms, but other sources such as plant material, insects, algae, cyanobacteria and fungi are used as well. Carbon and nitrogen are the most significant environmental factors influencing the pigments in cyanobacteria. Glucose, a source of energy and a metabolism regulator, differently affected the pigment contents, chlorophyll-a, phycocyanin, allophycocyanin and E- caroten in the cells. The present study aimed to investigate the effects of various glucose concentrations (10-320 mM) on biomass, chlorophyll-a, phycocyanin, allophycocyanin and E- caroten, of Anabaena sp. The maximum pigment contents were estimated at 10 mM glucose concentration. But, in the high concentrations of glucose are more than 40 mM, biomasses and pigment contents of this strain were severely reduced. Anabaena sp. GO10 was completely supressed by 60 mM and higher glucose concentrations. In this study show that, glucose use as carbon source for pigment production. There has been little information published on glucose applications of Cyanobacteriae pigments.

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