Kolesistektomi yapılan hastaların safra örneklerinden izole edilen mikroorganizmalar ve antibiyotiklere duyarlılıkları

Amaç: Safra kesesinde oluşan taşlar inflamasyon ve tıkanmaya bağlı olarak kese hidîopsuna neden olabilmekte, bunun sonucunda bakteriyel enfeksiyonlar gelişebilmektedir. Çalışmada bu durumun ortaya konulması amacıyla safra kesesi taşı nedeniyle ameliyat edilen hastalarda safra örnekleri alınarak bakteriyolojik açıdan incelenmiştir. Yöntem: Alınan safra örnekleri aerobik ve anaerobik olarak değerlendirilmiş, üreyen aerob ve anaerob kültürlerdeki patojenlerin identifikasyonu ve 37 antibiyotiğe karşı duyarlılıklarının belirlenmesinde Sceptor (Becton Oickinson-USA) panelleri kullanılmıştır. Bulgular: Çalışmada 41 safra örneğinden 33'ünde'(% 80.5) bakteriyel öreme saptanmıştır. Bu örneklerden 20'sinde (% 60.6) Gram negatif aerob [Escherichia coli (5), Enterobacter ctoacae (4), Acinetobacter baumannii (3), Pseudomonas aeruginosa (3), Stenotrophomonas maltophilia (1), Enterobacter agglomerans (1), Klebsiella pneumoniae (1), Serratia odorifera (1) ve Edvvardsiella ictaluri (1)], 8'inde (% 24.2) Gram pozitif aerob bakteriler [Staphylococcus aureus (1), Koagülaz negatif stafilokoklar (KNS) (5) ve Streptococcus agalactiae (2)], 4'ünde (% 12.1) anaerob bakteriler [Ruminococcus productus (2), Bacteroides ovatus (2)], bir örnekte ise (% 3.1) Candida alblcans izole edilmiştir. Sonuç: izole edilen bakterilere karşı yapılan antibiyotik duyarlılık testi sonucunda, en etkili antibiyotiklerin Gram negatiflere karşı amikasin ve imipenem, Gram pozitif bakterilere karşı vankomisin, oksasilin ve ampisilin-sulbaktam, anaeroblara karşı ise kloramfenikol olduğu tespit edilmiştir.

Microorganisms isolated from bile samples of the patients who had cholesistectomy and their antibiotics susceptibilities

Objective: Gallstones forming in the gali bladder cause inflammation and obstruction, and as a result bacterial infections may develop. in this study, bile samples of the patients who were operated for gallstones were collected and bacteriologically evaluated. Methods: Bile samples were investigated both aerobically and anaerobically, and yielded pathogens vvere identified and their antibiotic susceptibilities were detected by Sceptor (Becton Dickinson-USA)systems. Results: in this study, from 41 bile samples, in 33 samples (80.5%) bacterial yielding vvere detected. From 20 samples (60.6%) Gram negative aerobs [Escherichia coli (5), Enterobacter cloacae (4), Acinetobacter baumannii (3), Pseudomonas aeruginosa (3), Stenotrophomonas mattophilia (1), Enterobacter agglomerans (1), Klebsiella pneumoniae (1), Serratia odorifera (1) and Edwardsiella ictaluri (î)], in 8 samples (24.2%) Gram positive aerob bacteria [Staphylococcus aureus (1), Coagulase negative staphylococci (CNS) (5) and Streptococcus agalactiae (2)], in 4 samples (12.1%) anaerob bacteria [Ruminococcus productus (2), Bacteroides ovatus (2)] and in 1 sample (3.1%) Candida alblcans vvere isolated. Conclusions: As a result of the antibiotic susceptibility test performed on the isolated bacteria, amicasin and imipenem to Gram negatives, vancomycin, oxacilin and ampicilin-sulbactam to Gram positives, and chloramphenicol to anaerobs vvere detected as the most effective antibiotics.

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