İnsan eritrosit glukoz 6-fosfat dehidrogenaz enzim aktivitesi üzerine bazı antibiyotiklerin in vitro etkileri

Amaç : İnsan eritrositlerinden elde edilen glukoz 6-fosfat dehidrogenaz enzimi üzerine bazı antibiyotiklerin inhibisyon etkilerini araştırmak. Yöntem : Bu amaçla önce eritrosit glukoz 6-fosfat dehidrogenaz enzimi, 2',5'-ADP Sepharose 4B jelinin kullanıldığı afinite kromatografisi vasıtasıyla % 28 verimle 13.654 kat saflaştırıldı. Saflaştırma esnasında sıcaklık +2 o C'de kontrol altında tutuldu. Enzim aktivitesi Beutler metoduna göre 340 nm'de spektrofotometrik olarak belirlendi ve bütün kinetik çalışmalarda bu metod uygulandı. İlaç olarak seftriakson disodyum trisesquihidrat, penicillin G potasyum, amoksisilin sodyum kullanıldı. Bulgular : Bu ilaçların glukoz 6-fosfat dehidrogenaz enzimi üzerinde inhibisyon etkileri olduğu tesbit edildi. İnhibisyon etkisi gösteren bu ilaçlar için % aktivite-[I] grafikleri çizilerek I 50 değerleri bulundu. Sonuç : Glukoz 6-fosfat dehidrogenaz eksikliği olmayan sağlıklı kişilerde bu ilaçların kullanılması fazla sakıncalı olmamakla birlikte, glukoz 6-fosfat dehidrogenaz enzim eksikliği olan kişilerde, özellikle seftriakson disodyum trisesquihidrat kullanılmasının ciddi sağlık problemlerine yol açabilir.

In vitro effects of some antibiotics on enzymatic activity of human erythrocytes glucose 6-phosphate dehydrogenase

Objective : Inhibitory effects of some antibiotics on glucose 6-phosphate dehydrogenase enzyme isolated from human erythrocytes was researched. Methods : Erythrocyte glucose 6-phosphate dehydrogenase was purified 13.654 times with 28% yield by affinity chromatography in which 2',5'-ADP Sepharose 4B gel was used. During purification process, temperature was stabilized at +2 o C.Enzyme activity was determined by using spectrophotometer at 340 nm according to Beutler method. Ceftriaxone disodium trisesquihydrate, penicillin G potassium, amoxicillin sodium were used as antibiotics. Results : It was understood that all these drugs have inhibitory effects on glucose 6-phosphate dehydrogenase enzyme. I 50 values were found for these antibiotics by drawing % activity-[I] graphics. Conclusion : Although it does not cause any serious problem to use these drugs people who do not have G6PD deficiency, especially ceftriaxone disodium trisesquihydrate may cause serious health problems in G6PD deficient people.

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