Günün farklı zamanlarında yapılan futbol maçlarında oksidan-antioksidan dengenin incelenmesi

Amaç: Total oksidan seviye vücutta oksidatif stres düzeyinin arttığını gösteren ölçütlerden biridir. Total antioksidan seviye; total vücut antioksidan seviyesini göstermektedir. Bu çalışmanın amacı günün farklı zamanlarında yapılan akut egzersizlerin total oksidan ve antioksidan düzeyi üzerine olan etkilerini antrenmanlı ve sedanter deneklerde karşılaştırmalı olarak araştırmaktır. Böylece sirkadiyen ritim ve egzersizin; TAS ve TOS düzeyini nasıl değiştirdiği, antrenmanlı ve sedanter denekler arasında fark olup olmadığı belirlenecektir.Gereç ve Yöntem: Bu çalışmaya katılan 10 antrenmanlı ve 10 sedanter erkek denek sabah ve gece futbol maçı yapmışlardır. Maçtan önce ve maçtan hemen sonra venöz kan örnekleri alınmıştır. Serum total oksidan ve antioksidan düzeyi ELISA yöntemi kullanılarak analiz edilmiştir. Bulgular: Total antioksidandüzeyiantrenmanlıvesedanterdeneklerdesabah (1.03±0.08 mmol/L'den 0.91±0.06 mmol/L'ye; 0.98±0.08 mmol/L'den 0.83±0.1 mmol/L'ye; sırasıyla) vegece (0.94±0.1 mmol/L'den 0.82±0.09 mmol/L'ye; 1.11±0.16 mmol/L'den 0.96±0.10 mmol/L'ye; sırasıyla)istatistikselolarakanlamlıorandaazalmıştır (p < 0.01). Total oksidandüzeyiantrenmanlıvesedanterdeneklerdesabah (14.96±4.3 µmol/L'den 18.01±8.6 µmol/L'ye; 13.65±2.7 µmol/L'den 18.33±2.9 µmol/L'ye; sırasıyla) vegece (13.62±3.2 µmol/L'den 19.33±6.3 µmol/L'ye; 14.83±4.2 µmol/L'den 25.79±10.4 µmol/L'ye; sırasıyla) istatistiksel olarak anlamlı oranda artmıştır (p < 0.01). Sonuç: Bu çalışma sonuçları akut egzersizin TAS düzeyini azaltırken TOS düzeyini artırdığını göstermektedir. Bunun sonucunda akut egzersiz sırasında oksidatif stresin arttığı ve egzersiz zamanının önemi açığa çıkmaktadır.

Objective: Total oxidantlevelsare a measurethatshowingincreasedlevels of oxidativestress in body. Total antioxidantlevelsareshowedlevels of total body antioxidantlevels.Aim of thisstudy is investigatedeffects on levels of total oxidantandantioxidantlevelsthatperformeddifferenttimesacuteexercise as comparatively in trainedanduntrainedsubjects. Circadianrhythmandexercisewilldetermine how changing total oxidantandantioxidantlevelsandwhetherdifferencebetweentrainedanduntrainedsubjects. Material and Methods: Ten trained and ten untrained subjects participated this study that performed playing soccer match in morning and night. Pre and post match venous blood samples were taken. Serum total oxidant and antioxidant levels were analysed using Enzyme Linked-Immunosorbent Assay.Results: Total antioxidant levels were decreased significantly in morning (from 1.03±0.08 mmol/L to 0.91±0.06 mmol/L; from 0.98±0.08 mmol/L to 0.83±0.1 mmol/L; respectively) and night (from 0.94±0.1 mmol/L to 0.82±0.09 mmol/L; from 1.11±0.16 mmol/L to 0.96±0.10 mmol/L; respectively) in trained and untrained subjects (p < 0.01). Total oxidant levels were increased significantly in morning (from 14.96±4.3 µmol/Lto18.01±8.6 µmol/L; from13.65±2.7 µmol/L to 18.33±2.9 µmol/L; respectively) and night (from 13.62±3.2 µmol/Lto19.33±6.3 µmol/L; from14.83±4.2 µmol/L to 25.79±10.4 µmol/L; respectively) in trained and untrained subjects (p < 0.01). Conclusion: Thisstudydocumentsshoweddecreasing total antioxidantlevelsincreasing total oxidantlevelsduringacuteexercise. Thereby, thisresultshavebringoutintotheopenincreasedoxidativestressduringacuteexerciseandimportant of exercisetiming.

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