ERCAN ÖZDEMİR, Ayşe DEMİRKAZIK, Ahmet Sevki TASKIRAN, OLCA KILINÇ, GÖKHAN ARSLAN

Nitrik oksit sentaz (NOS) inhibitörlerinin sıçanlarda çok düşük frekanslı manyetik alanın indüklediği analjezi üzerine etkileri

Amaç: Elektromanyetik alanın (EMA) farklı ağrı türlerini azalttığı bilinmektedir. Bununla birlikte, manyetik alanın analjezik etki mekanizması tam olarak anlaşılamamıştır. Bu çalışmanın amacı, nitrik oksit sentaz (NOS) inhibitörlerinin sıçanlarda çok düşük frekanslı EMA maruziyeti ile oluşan analjezi üzerine etkilerini araştırmaktır.Gereç ve Yöntem: Bu çalışmada 72 yetişkin erkek Wistar albino sıçan (yaklaşık 230 ± 12 g ağırlığında) kullanıldı. Sıçanlar, 22 ± 2 °C oda sıcaklığında, 12 saat aydınlık/karanlık siklusun sağlandığı ve ses yalıtımı olan ortamda tutuldu. Elektromanyetik alan (50 Hz), her gün dört defa 30 dakika süre ve 15 dakika aralıklar ile 15 gün boyunca uygulandı. Analjezik etki ölçümü tail-flick ve hot-plate testleri ile gerçekleştirildi. Analjezi testinden önce sıçanlara nitrik oksit donörü SNAP (30 mg/kg) ve NOS inhibitörleri L-NAME (40 mg/kg) ve 7-NI (25 mg/kg) intraperitoneal olarak enjekte edildi. Verilerin istatistiksel analizinde varyans analizi (iki yönlü ANOVA) kullanılmış ve çoklu karşılaştırma Tukey testleri ile yapıldı. İstatistiksel olarak anlamlılık düzeyi p

Objective: It is known that the electromagnetic field (EMF) reduces the different types of pain. However, the mechanism of magnetic field analgesia is not fully understood. Our aim of this study is to investigate the effects of nitric oxide synthase (NOS) inhibitors on analgesia induced by extremely low frequency EMF in rats.Material and Methods: In this study were used 72 adult male Wistar albino rats (approximately 230 ± 12 g). The rats were provided environment where is at 22 ± 2 °C room temperature, 12-hour light/dark cycle and insulated from sound. The application of electromagnetic field (50 Hz), the same times for 30 minutes each day for 15 days, and a total of four times every 15 minute intervals. The analgesic effect measurement was performed by tail-flick and hot-plate tests. Prior to analgesia test, nitric oxide donor SNAP (30 mg/kg) and NOS inhibitors L-NAME (40 mg/kg) and 7-NI (25 mg/kg) were injected intraperitoneally in rats. In the statistical analyzes of the data, analysis of variance (two-way ANOVA) was used and the multiple comparison determined by Tukey tests. The level of statistically significant was expressed p <0.05.Results: Analgesia test results indicated that the maximum analgesic effect of elektromagnetic field produces in 5 mT and on day 7. Administration of L-NAME and 7-NI in rats exposed to a magnetic field the analgesic effects were significantly higher than EMF group rats (p<0.05). On the contrary, administration of SNAP in rats exposed to a magnetic field the analgesic effects significantly reduced compared to the EMF group (p <0.05).Conclusion: Obtained data suggested that the administration of L-NAME and 7-NI increased analgesic efficacy subjected to electromagnetic field in rats, whereas the administration of SNAP reduced the analgesic activity.

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