Anestezi cihazı ortamı ve volatil anesteziklerin Candida albicans’ın üreme hızına etkileri

Amaç: Maske, konnektör, tüp ve nemli solunum devreleri gibi anestezi ekipmanları infeksiyon vekontaminasyon için iyi bir ortam sağlarlar. Fakat volatil anesteziklerle anestetize edilen hastalarda kontaminasyon beklendiği kadar sık değildir. Biz bu çalışmada, halotan, izofluran ve sevofluranın ve anestezi cihazı ortamının in vitro şartlarda C. albicans üreme hızı üzerine olan etkilerini araştırmayı amaçladık. Yöntem: C. albicans ışık kırıcılığı, spektrofotometre ile 450 nµ’da ölçülerek giriş değeri olarak kaydedildi. Daha sonra C. albicans bir ve iki MAC (Minimum alveolar konsantrasyon) halotan, isofluran ve sevoflurana % 50 oksijenli anestezi cihazı ortamında bir, iki, üç ve dört saat süreyle maruz bırakıldı. C. Albicans’ın işlem sonundaki spektrofotometredeki ölçüm değeri çıkış değeri olarak kaydedildi. Sonuç: Volatil anestezikler ve anestezi cihazı ortamı C. albicans üreme hızını inhibe etti.

Anahtar Kelimeler:

Halotan, Anestezi, Anestezikler, Candida albicans, Donanım kontaminasyonu, İzofluran

Effects of anesthesia equipment conditions and volatile anesthetics on the growth rate of Candida albicans

Objective: Anesthesia equipment such as masks, connectors, tubes and humid breathing circuits provide a good envoirment for infection and contamination. But contamination is not as frequent as expected in patients anesthetized with volatile anesthetics. In this study, we aimed to study the in vitro effects of halothane, isoflurane and sevoflurane, anesthesia equipment conditions on the growth rate of C. albicans. Methods: The light absorbancy of C. albicans was recorded as basic value at 450 nµ with a spectrophotometer. C. albicans inoculated in buyyon was subjected to one and two MAC halothane, isoflurane and sevoflurane for one, two, three and four hours in 50% oxygen within the anesthesia machine. The light absorbancy of the C. albicans was recorded at the end of the exposure to volatile anesthetics. A control group was studied without volatile anesthetics. Conclusion: Volatile anesthetics and anesthesia equipment conditions inhibited the growth rate of C. albicans.

Keywords:

Halothane, Anesthesia, Anesthetics, Candida albicans, Equipment Contamination, Isoflurane,

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1. Molliex S, Montraves P, Dureuil B. Halogenated anesthetics inhibit Pseudomonas aeruginosa growth in culture conditions reproducing the alveolar environment. Anesthesia Analgesia 1998;86:1075-8. 2. Asehnoune K, Cruaud P, Paries J, Gorce P, Pourriat JL. Effects of isoflurane on bacterial growth. Eur J Anaesth 2000;17:289-94. 3. Orkin FK. Anesthetic systems. In: Miller RD, editor. Anesthesia. 2nd ed. New York, Churchill Livingstone, 1986;147. 4. Larsen B, Snyder L, Galask RP. Bacterial growth inhibition in human amniotic fluid. Am J Gynecol 1974;119:492-6. 5. Bridget WS, Nunn JF. The effect of halotan on bacterial growth rate. Br J Anaesth 1971;43:919-25. 6. Gary C, Moulin MS, Albert J. The anesthesia machine and circle system are not likely to be sources of bacterial contamination. Anesthesiology 1977;47:353-8. 7. Horton JN, Sussman M, Mushin WW. The antibacterial action of anaesthetic vapours. Br J Anaesth 1970;42:483-7. 8. Slade JM. Bacterial growth in isoflurane vapour. Anaesthesia 1993;48:1053-4. 9. Wardley-Smith B, Nunn JF. The effect of halothane on bacterial growth rate. Br J Anaesth 1971;43:919-25. 10. Barry PP, Patement B, Dubeau M. Recherches sur l’activité antibactérienne de certains agents anesthesiques. Canadian Anaesth Soc J 1964;11:640-4. 11. Gary C, Moulin MS, Hedley-Whyte J. Bacterial interactions between anesthesiologists their patients and equipment. Anesthesiology 1982;57;37-41. 12. Giorgi A, Parodi F, Piacenza G, Montellini E, Sahio M, Cremonte LG, et al. Antibacterial and antifungal activity of isoflurane and common anesthetic gases. Minerva Med 1986;10:42-3. 13. Topal A, Duman A, Öğün C, Şahin TK, Erol A, Arslan U, ve ark. Volatil anesteziklerin bakteri üreme hızına etkileri. Genel Tıp Derg 2002;3:95-100.