Walaa Najm ABOOD, Ali Ibrahim ALİ EZZY AL

Indirect Heamagglutination as an Immunodiagnostic Technique for Cystic Echinococcosis in Iraqi Patients

Amaç: Irak'ta kistik ekinokokkozis tanısı için indirekt hemaglutinasyonun (IHA) etkinliğinin değerlendirilmesi ve hastalığın ortaya çıkmasında yaş ve cinsiyetin ilişkisinin araştırılmasıdır. Gereç ve Yöntem: IHA tekniği kistik ekinokokkus antijeni ile kaplanmış sensitize koyun eritositleri ile uygulanmıştır. Ki kare ve Pearson korelasyon testi analizlerde kullanılmıştır Bulgular: Bu çalışmaya kistik ekinokokkus kliniği olan toplam 202 hasta dahil edilmiştir. Ortalama yaş 31.64±8.98 yıldır. En düşük yaş 18 ve en ileri yaş ise 70 yaştır. Erkekler, hastaların %50'sini oluşturmakla birlikte ortalama yaş 33.50±7.8 yıldır. Kadınlarda ise ortalama yaş 29.78±9.6 yıl olarak saptanmıştır. Pozitif olgular %19.3 iken negatif olanlar %80.7 idi. Pozitif olgular kadınlar arasında daha yüksek orandayken (%22.8) erkeklerde ise %15.8 olarak bulunmuş ve aralarında anlamlı bir ilişki bulunmamıştır. Ayrıca, IHA sonuçları ve cinsiyet arasında da korelasyon yoktur (p = 0.214). Hastaların çoğunluğunun yaş aralığı; 24-30 yıl, 31-36 yıl ve 18-23 yıl olarak değişmektedir. Erkeler arasında, en yüksek pozitif olgu 31-36 yıl arasında diğerleri ise 24-30 yıl ve 49-54 yıl arasındadır. Kadınlar arasında ise en yüksek pozitif olgu 24-30 yıl arasında ve sonra 37-42 yıl ve 18-23 yıl gelmektedir. Yaş grupları ve cinsiyet arasında anlamlı ilişki saptanırken (p= 0.001), cinsiyete göre yaş grupları ve IHA sonuçları arasında ise anlamlı bir ilişki bulunmamıştır (p= 0.268). Sonuç: IHA, hızlı, güvenilir ve yeterli bir teknik olduğu için yüksek sayıdaki örneğin aynı anda taranmasına imkan verir.Teknik, iyi eğitimli personele ihtiyaç duymaz. Genç yaş gruplarında, kadınların kistik ekinokokkusa erkeklerden daha fazla yatkın oldukları gözlenmiştir.

Iraklı Hastalarda Kistik Ekinokokkozisin İmmunodiagnostik Teknikle Tanısı için İndirekt Hemaglutinasyonun Kullanılması

Objective: The aim is to determine the effectiveness of indirect Haemagglutination (IHA) as diagnostic techniques for Cystic Echinococcosis in Iraqi and the possible correlation of age and gender in disease occurrence. Material and Methods: IHA technique using sensitized sheep red blood cells coated with an Echinococcus granulosus antigen. A chi square, Pearson correlation used for analysis of categorical variables. Results: Patients presented with clinical manifestation of hydatidosis (202) selectively enrolled in this study. The mean age was 31.64±8.98 years. The minimum age was 18 years and the maximum age was 70 years. Males represent 50%, with the mean age of 33.50±7. 8 years. The rest of patients were females with a mean age of 29.78±9. 6 years. Positive cases represent 19.3%, and the 80.7% were negative. The number of positive cases was higher among females (22.8%) compared with males (15.8%) with no significant difference (p= 0.212) or correlation between gender and IHA results (p = 0.214) .The majority of patients had a the rage of 24-30 years, 31-36 years and 18-23 years. Among males, a higher number of positive cases at 31-36 years followed by 24-30 years and 49-54 years. Among females, a higher number of positive cases with 24-30 years of age was followed by those with 37-42 years of age and 18-23 years of age. There was a significant difference (p= 0.001) between age groups according to gender without a significant correlation between the age group and IHA results according to gender (p= 0.268). Conclusion: The IHA is considered as rapid, reliable and adequate technique that makes it possible to process a large number of samples simultaneously. It does not require highly trained technical personnel. Females appear to be more susceptible to CE than males especially in younger age groups.

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