Sibel BAYRAK, Yasemin AKSOY, Bilge PEHLİVANOĞLU, Dicle Zeynep BALKANCI

Effects of transient hypotension on glutathione levels and lipid peroxidation in the liver during pregnancy in rats

Amaç: Gebelik, kan basıncının düzenlenmesi veya çesitli organlardaki oksidan durum gibi çok sayıda farklı vücut sistemlerinde fizyolojik olarak önemli ve çoğu zaman geri dönüsümlü değisikliklere neden olur. Sıklıkla rastlanmasına karsın geçici hipotansiyonun anne ve fetus üzerindeki etkileri çok fazla çalısılmamıstır. Hipotansiyon sırasında pek çok dokunun kan akımında ve oksijenizasyonunda değisiklikler gözlenir. Karaciğer hipoksiye duyarlıdır ve oksidan dengedeki değisiklikler kan gibi diğer dokulardaki değisikliklerin öncülü olabilir. Bu çalısmada literatür bilgilerine dayanarak, gebelerde geçici hipotansiyonun karaciğerdeki oksidan strese etkisini arastırdık. Gereç ve Yöntem: 28 adet üç aylık Wistar albino sıçan gebe olmayan kontrol (GOK), gebe olmayan hipotansiyon (GOH), gebe kontrol (GK) ve gebe hipotansiyon (GH) olmak üzere dört gruba ayrıldı. GK ve GH grupları gebeliğin 15. gününde GOK ve GOH grupları ise laboratuvara geldikten bir hafta sonra sağ femoral arter kateterizasyonu yapılarak, kan alınması yoluyla 30 dakikalık hipotansiyona maruz bırakıldı. Cerrahiden 48 saat sonra hayvanlar feda edildi ve karaciğer dokusu çıkarılarak malonyldialdehid (MDA) ve redükte glutatyon (GSH) düzeyleri için biyokimyasal analizler yapılana kadar -80° C’da saklandı. Bulgular: MDA düzeyleri gebe ve hipotansiyon gruplarında kontrol hayvanlarına göre anlamlı olarak yüksekti. Tüm gruplar arasında GH grubunda en düsük GSH ve en yüksek MDA düzeyleri ölçüldü (p

Sıçanlarda gebelik sırasında geçirilen geçici hipotansiyonun karaciğer lipit peroksidasyonu ve glutatyon düzeyine etkisi

Objective: Pregnancy is associated with physiologically reversible but important changes in various body systems like blood pressure regulation or oxidant status in various organs. The effects of transient hypotension on the fetus and mother are not studied extensively although experienced frequently. During hypotension, blood flow of many tissues is altered. The liver is vulnerable to hypoxia and the affected oxidant balance may lead to changes in other tissues, including the blood. On the basis of these accumulated data, in the present study we tested the effects of transient hypotension on oxidant stress in the liver in pregnant rats. Materials and Methods: Twenty-eight 3-month-old female Wistar-albino rats were divided into virgin control (VC), virgin hypotension (VH), pregnant control (PC), and pregnant hypotension (PH) groups. On day 15 of pregnancy in the PH and PC groups and a week after admission to the laboratory in the VH and VC groups the right femoral artery was catheterized and hypotension was created for 30 minutes by blood withdrawal. Forty-eight hours after the surgery all the animals were sacrificed and liver tissues were stored at -80 °C until biochemical analysis for malonyldialdehyde (MDA) and reduced glutathione (GSH) was performed. Results: MDA levels were higher in the pregnant and hypotensive groups compared to the control animals. Amongst all the groups, the PH group had the lowest GSH and the highest MDA levels (p

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1. Kumar CA, Das UN. Oxidant stress in preeclampsia and essential hypertension. J Assoc Physicians India 2002; 50:1372-1375.
2. Sainz RM, Reiter RJ, Mayo JC, et al. Changes in lipid peroxidation during pregnancy and after delivery in rats: effect of pinealectomy. J Reprod Fertil 2000; 119:143-149.
3. Voss A, Baumert M, Baier V, Stepan H, Walther T, Faber R. Autonomic cardiovascular control in pregnancies with abnormal uterine perfusion. Am J Hypert 2006; 19:306-312.
4. Silver HM, Tahvanainen KUO, Kuusela TA, Eckberg DL. Comparison of vagal baroreflex functions in nonpregnant women and in women with normal pregnancy, preeclampsia, or gestational hypertension. Am J Obstet Gynecol 2001; 184:1189-1195.
5. Hines T, Beauchamp T, Rice C. Baroreflex control of sympathetic nerve activity in hypertensive pregnant rats with reduced uterine perfusion. Hypert Preg 2007; 26:303–314.
6. Eliseev OM. Cardiovascular diseases and pregnancy. New York: Springer-Verlag 1988.
7. Brooks VL, Keil LC. Hemorrhage decreases arterial pressure sooner in pregnant compared with nonpregnant dogs: role of baroreflex. Am J Physiol 1994; 266:H1610-1619.
8. Kumar CA, Das UN. Lipid peroxides, antioxidants and nitric oxide in patients with preeclempsia and essential hypertension. Med Sci Monit 2000; 6:901-907.
9. Ng PH, Walters WAW. The effects of chronic maternal hypotension during pregnancy. Aust NZJ Obstet Gynaecol 1992; 32:14-16.
10. Chen A, Basso O. Does low maternal blood pressure during pregnancy increase the risk of perinatal death? Epidemiology 2007;18:619-622.
11. Rensing H, Bauer I, Peters I, et al. Role of reactive oxygen species for hepatocellular injury and heme oxygenase-1 gene expression after hemorrhage and resuscitation. Shock 1999; 12:300-308.
12. Raza H, John A. Glutathione metabolism and oxidative stress in neonatal rat tissues from streptozotocin-induced diabetic mothers. Diabetes Metab Res Rev 2004; 20:72-78.
13. Altavilla D, Saita A, Guarini S, Galeano M, Squadrito G et. al. Oxidative stress causes nuclear factor-kappa B activation in acute hypovolemic hemorrhagic shock. Free Rad Biol Med 2001; 30:1055-1066.
4. Mover-Lev H, Amos A. Changes in enzymatic antioxidant activity in pregnant rats exposed to hyperoxia or hypoxia. Comp Biochem Physiol 1997; 118:35335-35339.
15. Regel G, Grotz M, Weltner T, Sturm JA, Tscherne H. Pattern of organ failure following severe trauma. W J Surgery 1996; 20:422-429.
16. Paxian M, Bauer I Rensing H, et al. Recovery of hepatocellular ATP and pericentral apoptosis after hemorrhage and resuscitation. FASEB J 2003; 17:993-1002.
17. Codes of Practice for the Care and Use of Animals for Scientific Purposes. Available from http://www.nhmrc.gov.au/publications/synopses/ ea16syn.htm. Accessed in 28 February 2008.
18. Bergman AS, Sinelnikova MP. Functional-morphological changes of placental tissue in women with hypotensive syndrome, Akush Ginekol 1973; 49:45-48.
19. Steer PJ, Little MP, Kold-Jensen T, Chapple J, Elliott P. Maternal blood pressure in pregnancy, birth weight, and perinatal mortality in first births: prospective study. Br Med J 2004; 329: 1312-1347.
20. Mihara M, Uchiyama M. Determination of malonyldialdehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1977;86:271.
21. Tietz F. Enzymic method of quantitative determination of nanogram amounts of total and oxidized glutathione: application to mammalian blood and other tissues. Anal Biochem 1969; 27:502.
22. Miller RS, Thompson ML, Williams MA. Trimester-specific blood pressure levels in relation to maternal pre-pregnancy body mass index. Paediatr Perinatal Epidemiol 2007; 21:487-494.
23. Hermida RC, Ayala DE, Iglesias M. Predictable blood pressure variability in healthy and complicated pregnancies. Hypertension 2001;38:736-741.
24. Karaman A, Fadillioglu E, Turkmen E, Tas E, Yilmaz Z. Protective effects of leflunomide against ischemia-reperfusion injury of the rat liver. Pediatr Surg Int 2006; 22:428–434.
25. Fondevila C, Shen X, Tsuchiyashi S, Yamashita K et al. Biliverdin therapy protects rat livers from ischemia and reperfusion injury. Hepatology 2004; 40:1333–1341.
26. Jaeschke H. Reactive oxygen and mechanisms of inflammatory liver injury. J Gastroenterol Hepatol 2000; 15:718–724.
27. Kurcer Z, Oguz E, Iraz M, Fadillioglu E, et al. Melatonin improves methanol intoxication-induced oxidative liver injury in rats. J Pineal Res 2007; 43:42–49.
28. Yoshioka T, Motoyama H, Yamasaki F, Ando M, Takehara Y, Yamasaki M. Lipid peroxidation and vitamin E levels during pregnancy in rats. Biol Neonate 1987; 52:223-231.
29. Wisdom SJ, Wilson R, McKillop JH, Walker J. Antioxidant systems in normal pregnancy and in pregnancy-induced hypertension. Am J Obstet Gynecol 1991; 165:1701-1704.
30. Kiray M, Ergur BU, Bagriyanik A, Pekcetin C, Aksu I, Buldan Z. Suppression of apoptosis and oxidative stress by deprenyl and estradiol in aged rat liver. Acta histochemica 2007; 109:480–485.
31. Kankofer M, Radzki RP, Bienko M, Albera E. Anti-oxidative/oxidative status of rat liver after ovariectomy J Vet Med 2007; 54: 225–229.
32. Yamamasu S, Sato EF, Ogita S, Inoue M. Role of glutathione metabolism and apoptosis in the regression of liver hemopoiesis. Free Radic Biol Med 1997; 23:100-109.
33. Walsh SW, Wang Y. Deficient glutathione peroxidase activity in preeclampsia is associated with increased placental production of thromboxane and lipid peroxides. Am J Obstet Gynecol 1993; 169:1456-1461.
34. Kundu KT, Hille R, Velayutham M, Zweier JL. Characterization of superoxide production from aldehyde oxidase: An important source of oxidants in biological tissues. Arch Biochem Biophys 2007; 460:113–121.