Effect of alcohol on lipid levels in the brains of rats

Amaç: Biz çalısmamızda alkol tüketiminin sıçan beyninde kolesterol, trigliserid ve fosfolipid seviyelerine etkisini arastırdık. Gereç ve yöntem: Bu amaçla 24 Sprague-Dawley disi sıçan, alkol (n=12) ve kontrol (n=12) grubu olmak üzere iki gruba ayrıldı. Her iki grup çalısma süresince normal laboratuvar yemi ile beslendi. Kontrol grubuna normal musluk suyu verilirken alkol grubunun içme sularına %15 (v/v) oranında etanol ilave edildi. İki aylık beslenme süresinden sonra sıçanlar ketamin anestezisi altında dekapite edildi ve beyin dokuları çıkarılarak %0.1 digitonin içeren, 0.1 M soğuk fosfat tamponu içinde homojenize edildi. Homojenatlar santrifüj edildikten sonra süpernatanları alındı ve rutin metodlarla kolesterol, trigliserid ve fosfolipid düzeyleri çalısıldı. Bulgular: Alkol grubunda beyin kolesterol, trigliserid ve fosfolipid düzeyleri sırasıyla 29,36 ± 1,68, 39.81 ± 8,5 ve 2,91 ± 0,82 kontrol grubunda ise 22,58 ± 2,29, 32,57 ± 3,7 ve 2,47 ± 0,64 mgr/gr protein olarak bulundu. Her iki gruba ait beyin kolesterol (p

Alkolün sıçanlarda beyin lipid seviyelerine etkisi

Purpose: To investigate the effects of alcohol consumption on rat brain cholesterol, triglyceride, and phospholipids levels. Materials and methods: The study was performed on 24 Sprague-Dawley rats of which 12 were selected as the control group and 12 as the alcohol group. Both groups were fed regular laboratory chow. In addition, the alcohol group rats were fed 15% (v/v) of ethanol in their drinking water for 2 months. Then, the rats were decapitated under ketamine anesthesia and the brain tissues were removed and homogenized in ice-cold 0.1 M phosphate buffer containing 1% digitonin. The tissues were centrifuged and brain cholesterol, triglyceride, and phospholipid levels of supernatants were measured using commercially available enzymatic kits. Results: Brain cholesterol, triglyceride, and phospholipid levels of the alcohol group were 29.36 ± 1.68, 39.81 ± 8.5, and 2.91 ± 0.82, respectively, whereas the same parameters of the control group were 22.58 ± 2.29, 32.57 ± 3.7, and 2.47 ± 0.64mg/ g protein, respectively. Brain cholesterol (p

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