Candan ÖZOĞUL, Deniz ERDOĞAN, Kubilay VİCDAN, Çiğdem ELMAS, Murat TAŞ

Investigation of the ultrastructure of the epididymis after application of oestrogen-free and oestrogen-rich diets

Amaç: Fitoöstrojenler, bitkilerde doğal olarak bulunan steroid yapıda olmayan östrojenlerdir. Bizim bu çalısmamızdaki amacımız, değisik yoğunluklarda fitoöstrojen içeren diyetin epididimis ince yapısı üzerindeki etkilerini elektron mikroskop düzeyinde arastırmaktır. Gereç ve Yöntem: Bu çalısmada fitoöstrojen içermeyen diyet ( Fito-0 grubu ), standart diyet ile aynı miktarda fitoöstrojen içeren ( Fito-500 yada kontrol grubu ) ve yüksek dozda fitoöstrojen içeren ( Fito-1000 yada fito zengin grubu ) diyetin etkilerinin arastırılması planlandığı için üç ayrı diyet hazırlandı. 18 adet 21 günlük Swiss Albino cinsi erkek fare sütten kesilir kesilmez hiçbir ek gıda almadan temin edildi ve rastgele her bir grup 6 fare içerecek sekilde 3 gruba ayrıldılar. Fareler 63 günlük ( 9 hafta ) oluncaya değin bu ortamda üç ayrı diyetle beslenecek sekilde bakıldılar. Carl Zeiss EM 900 elektron mikroskobunda epididimisin ince yapısı incelendi. Bulgular: Fito-0 grubunda; epididimise ait bazal hücreler (B) ve esas hücreler (E) normal yapılarıyla izlendiler. Fito-500 grubunda; bazal hücreler genel yapılarını korurken , esas hücrelerin iki farklı tipte yapısal özellik gösterdiği ilgiyi çekti. Hücreler açık (A) ve koyu (K) renk sitoplazmalıydı. Fito-1000 grubunda; koyu ve açık renk esas hücreler ve bazal hücreler yaygın dejenerasyon ile izleniyordu. Sonuç: Sonuç olarak, diyetle yüksek miktarda fitoöstrojen alınması epididimis hücrelerinde dejeneratif değisikliklere yol açmıstır ve bu erkek farelerde infertiliteye neden olabilir.

Östrojenden yoksun ve zengin diyet uygulamasında epididimis’ın ince yapısının incelenmesi

Purpose: Phytoestrogens are oestrogens that are non-steroidal and are naturally found in plants. This study examined the effects of a phytoestrogencontaining diet on the thin layer of epididymis at electron microscopic level. Materials and Methods: This study was carried out to investigate the effects of a non-phytoestrogen diet (Phyto-0 group), a standard diet containing the same amount of phytoestrogen (Phyto-500 or control group), and a diet with a high dose of phytoestrogen (Phyto-1000 or phyto-rich group). Three different diets were prepared. Eighteen Swiss Albino type male mice 21 days old were obtained soon after weaning before they received any supplementary food and they were fed until they were 63 days old (9 weeks). For electron microscopy, tissue sections were processed and stained using the usual methods. Results: Basal cells and chief cells built-in in the basal epithelium of the epididymis were examined and found to have their normal structure in the Phyto-0 group. It was interesting that chief cells showed two different structural characteristics and basal cells preserved their general structure in the Phyto-500 group, while light and dark coloured chief cells and basal cells were examined and found to have diffuse degeneration in the Phyto-1000 group. Conclusion: Histological changes seen in the epididymis due to a high dose of phytoestrogen in the diet may cause infertility in male mice.

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