Nesrin SEYHAN, Aşkın IŞIMER, Cemal ÇEVİK, Ahmet AYDIN, Ayşe G. CANSEVEN

Effects of ambient elf magnetic fields: Variations in electrolyte levels in the brain and blood plasma

Amaç: Bu çalışmada, ELF frekanslı manyetik alanların beyin ve plazma elektrolit düzeylerini etkileyip etkilemediğini araştırmak amaçlanmıştır. Yöntem: Erkek, 250-300 g ağırlıkta kobaylar, sıcaklığı 23 °C, geomanyetik alanı 30 $mu$T (mikroTesla) olan laboratuarda plastik kafeslerde ve 12 saat gece-12 saat gündüz koşullarında muhafaza edildi. Kontrol ve deney grubu olmak üzere iki gruba ayrıldı;.kontrol grubu kobayları (n=l 1) aynı laboratuar şartlarında manyetik alana maruz bırakılmadan tutuldu. Deney grubu (n=24) ise günde 4 saat olmak üzere 5 gün boyunca 50 Hz, 2 mT manyetik alana maruz bırakıldı. Maruziyet tamamlandığında tüm hayvanların kan örnekleri enjektör ile doğrudan kalbe girerek alındı, bunu takiben hayvanlar eter ile bayıltıldı ve beyin dokuları çıkarıldı. Plazma be beyin Cu++, Zn++, Ca++ ve Mg++ konsantrasyonları atomik absorbsiyon tekniği ile Na+ ve K+ ise doğrudan plazmanın alev fotometre cihazına uygulanması yoluyla tesbit edildi. Bulgular: Plazma Na+, Ca++ ve Mg++ düzeylerinin manyetik alan etkisi ile artmış olduğu, Zn++ ve K+ seviyelerinin ise azalmış olduğu belirlendi. Ca++ seviyesindeki artış istatistiksel anlamda önemli bulundu. Cu++ düzeyi manyetik alandan etkilenmedi. Beyin dokusu Cu++, Zn++, Ca++ ve Mg++ konsantrasyonları artmış bulundu, ancak Zn++ ve Mg++'un manyetik alandan daha fazla etkilenmiş olduğu saptandı. Sonuç: ELF manyetik alanın beyin ve plazmada Ca++ miktarını etkilediği belirlendi. Dış kaynaklı alanların hedefinin hücre membranı olduğu ve ELF alanların canlı sistemlerle etkileşebilmesi için Ca++ tarafından düzenlenen mekanizmalara ihtiyaç duyulduğu yönünde bir genel inanış oluşmuştur. Bizim bulgularımız da bu hipotezi destekler görünmektedir.

Çevresel elf manyetik alanların etkileri: Beyin ve plazma elektrolit seviyelerinde değişimler

Purpose: To determine whether concentrations in (tie brain and plasma tissues are influenced by ELF magnetic fields.Methods: Male, 250-300 g guinea pigs were kept.under the laboratory conditions of 23 °C, a day and night cycle of 12 hours and an ambient geomagnetic field of 30 $mu$T (microTeslas) in plastic cages. The subjects were divided into, two groups: a control group (n=l 1) and an experimental group (n=24), which was exposed to a 50 Hz, 2 mT field for 4 hours/day for 5 days. The control subjects were handled in an identical manner without being exposed to any magnetic field. After the completion of the experiment, blood samples from the control and exposed animals were collected by cardiac puncture without hemolysis. The animals were sacrificed by ether inhalation in a closed box; then their brains were dissected out immediately. Cu++, Zn++, C.a++ and Mg++ concentrations in both the plasma and brain tissue of guinea pigs weie determined by flame atomic absorption. Na+ and K+ concentrations in the plasma were measured by direct application to flame photometry Results: Na+, Ca++ and Mg++ concentrations in the blood plasma increased while Zn++ and K+ concentrations decreased with the effect of the magnetic field. The increase in the Ca++ concentration was statistically significant The Cu++ concentration was not affected by magnetic field exposure The magnetic field, having a greater effect on Zn++ and Mg++ concentrations increased Cu++, Zn++, Ca++ and Mg++ concentrations in the brain tissue Conclusion: The ELF magnetic field altered the Ca++ concentration in the brain and plasma tissues. It is generally thought that the cell membrane is the first target of external fields, and calcium regulated activity is involvedin ELF field coupling to living systems. Our results appear consistent with this hypothesis.

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