Sivas Kangal ve Alman Kurt Köpeklerinde Canine Herpesvirus-1 Seroprevalansı

Bu çalışmada; 40 adet Alman Kurt ve 55 adet Sivas Kangal olmak üzere toplam 95 adet köpek kanserum örneğinde canine herpesvirus-1 (CHV-1) antikorlarının ırk, cinsiyet ve yaş ile ilişkisininbelirlenmesi amaçlanmıştır. Köpeklerden elde edilen serum örneklerinde CHV-1 antikor varlığıkomplement ilaveli virus nötralizasyon testi (VNT) ve ELISA tekniği ile araştırılmıştır. VNT ile 24hayvanda (%25.2) CHV-1 antikoru tespit edilmiştir. VNT ile seropozitif bulunan serum örnekleriELISA ile de pozitif bulunmuştur. Antikor pozitifliğinin cinsiyetle ilişkisi arasında anlamlı bir farkbulunamazken (P>0.05), Sivas Kangal köpeklerindeki seropozitiflik oranının Alman Kurtköpeklerindekinden daha fazla olduğu, seropozifliğin yaşla birlikte arttığı ve en yüksek oranda(%64.7) 5 yaş köpeklerde olduğu saptanmıştır.

Seroprevalence of Canine Herpesvirus-1 in Anatolian Shepherd Dogs and German Shepherd Dogs

This study was conducted to determine relationship among the antibody of canine herpesvirus-1 (CHV-1), breed, gender and age in a total of 95 dogs (55 Anatolian Shepherd Dogs and 40 German Shepherd dogs). CHV-1 antibody was detected by using virus neutralization test with added complement (VNT), and ELISA technique. CHV-1 antibody was detected in 24 dogs (25.2%) by VNT. Serum samples which were positive by VNT were also found positive by ELISA. There was no significant relationship between being antibody positive and gender (P>0.05), but Anatolian Shepherd dogs had higher serapositive ratio than German Shepherd dogs. The rate of the seropositivity increased by age and the highest was at age 5 (64.7%).

PDF

___

1. Kraft S, Evermann JF, McKeirnan AJ, Rigss M. The role of neonatal canine herpesvirus infection in mixed infections in older dogs. Compend Continuing Edu Practising Veterinarian 1986; 8: 688-694.
2. Hashimoto A, Hirai K, Suzuki Y, Fujimoto Y. Experimental transplacental transmission of canine herpesvirus in pregnant bitches during the second trimester of gestation. Am J Vet Res 1983; 44: 610-614.
3. Parzefall B, Ficher A, Blutke A, Schmahl W, Matiasek K. Naturally-occuring canine herpesvirus-1 infection of vestibular labyrinth and ganglion of dogs. Vet J 2011; 189:100-102.
4. Ronsse V, Verstegen J, Onclin K, et al. Risk factors and reproductive disorders associated with canine herpesvirus- 1 (CHV-1). Theriogenology 2004; 61: 619-636.
5. Okuda Y, Ishida K, Hashimoto A, et al. Virus reactivation in bitches with a medical history of herpesvirus infection. Am J Vet Res 1993; 54: 551-554.
6. Burr PD, Campbell MEM, Nicolson L, Onions DE. Detection of canine herpesvirus 1 in a wide range of tissues using the polymerase chain reaction. Vet Microbiol 1996; 53: 227-237.
7. Lebich M, Harder TC, Frey HR, et al. Comparative immunological characterization of type-specific and conserved B-cell epitopes of pinniped, felid and canid herpesviruses. Arch Virol 1994; 136: 335-347.
8. Bujko M, Sulovic V, Zivanovic V, Lako B, Dotlic R. Effect of progesterone and pregnancy on the replication of herpes simplex virus tip 2 in vivo. Clin Exp Obstet Gynecol 1998; 15: 34-7.
9. Ronsse V, Verstegen J, Onclin K, et al. Canine herpesvirus-1 (CHV-1): Clinical, serological and virological patterns in breeding colonies. Theriogenology 2005; 64: 61-74.
10. Reading MJ, Field HJ. Detection of high levels of canine herpesvirus-1 neutralising antibody in kennel dogs using a novel serum neutralisation test. Res Vet Sci 1999; 66: 273- 275.
11. Rijsewijk FAM, Luiten EJ, Daus FJ, Heijden RW, Oirschot JT. Prevalence of antibodies against canine herpesvirus 1 in dogs in The Netherlands in 1997-1998. Vet Microbiol 1999; 65: 1-7.
12. Ye ilba K, Yalçın E, Tuncer P, Yılmaz Z. Seroprevalance of canine herpesvirus-1 in Turkish dog population. Res Vet Sci 2012; 92: 36-39.
13. Özdamar K. SPSS ile Biyoistatistik. Yenilenmi 9. Baskı, Ankara: Sözkesen Matbaacılık Tic Ltd Sti, 2013.
14. Dahlbom M, Johnson M, Myllys V, Taponen J, Andersson M. Seroprevalance of canine herpesvirus-1 and Brucella canis in Finnish breeding kennels with and without reproductive problems. Rep Dom Ani 2009; 44: 128-131.
15. Ronsse V, Verstegen J, Onclin K, et al. Seroprevalance of canine herpesvirus-1 in the Belgian dog population. Rep Dom Ani 2002; 37: 299-304.
16. Nöthling JO, Hüssy D, Steckler D, Ackermann M. Seroprevalence of canine herpesvirus in breeding kennels in the Gauteng Provience of South Africa. Theriogenology 2008; 69: 276-282.
17. König M, Neiseke J, Thiel HJ. Prevalance of canine herpesvirus 1 (CHV-1) in German kennels. Tierearztliche Umschau 2004; 59: 559-565.
18. Takumi A, Kusanagi K, Tuchiya K, et al. Serodiagnosis of canine herpesvirus infection- development of an enzymelinked immunosorbent assay and its comparison with two improved methods of serum neutralization test. J Vet Sci 1990; 52: 241-250.