Hüseyin GÜNGÖR, Zeynep TUNA, Mahmut ŞAHİN, Haki KARA, Alper Serhat KUMRU

Sıçanlarda Sisplatin ile Oluşturulan Deneysel Testis Hasarı Modelinde Boldin veDekspantenolün Koruyucu Etkilerinin Araştırılması

Bu çalışma, sisplatinin neden olduğu olası testis hasarına karşı dekspantenol ve boldin'in koruyucu etkilerini belirlemek amacıyla yapılmıştır. Çalışmada 48 adet 4-5 aylık erkek Wistar rat (250-270 g) kullanıldı. Her grupta 6 hayvan olacak şekilde 8 grup oluşturuldu. Ratlara sisplatin (7 mg/kg) ile birlikte boldin (20 ve 40 mg/kg) ve dekspantenol (250 ve 500 mg/kg) uygulandı. 14. günün sonunda spermatolojik ve histopatolojik incelemelerle testis dokusu malondialdehit (MDA), süperoksit dismutaz (SOD), glutatyon peroksidaz (GSH-Px) ve katalaz (CAT) düzeyleri ölçüldü. Sisplatin grubunda sperm kalitesi ve motilitesinde anlamlı azalma, MDA'da anlamlı artış, SOD, GSH-Px, CAT düzeylerinde de anlamlı azalma gözlendi. MDA seviyeleri diğer gruplarda sisplatin grubuna göre anlamlı derecede azalmıştır. Tedavi gruplarında SOD, GSH-Px ve CAT enzim seviyeleri sisplatin grubuna göre arttı ve kontrol grubu düzeyine yaklaştı. Her iki maddenin yüksek dozlarının sisplatinle kombine uygulaması histopatolojik lezyonları azalttı. Dekspantenol ve boldinin her iki dozunun sisplatinle kombine uygulandığı gruplarda sisplatin grubuna göre anlamlı artışlar tespit edildi. Sonuç olarak, ratlarda sisplatinin indüklediği testis hasarına karşı dekspantenol ve boldin uygulamalarının koruyucu etkisinin olabileceği kanaatine varıldı.

Investigation of the Protective Effects of Dexpanthenol and Boldine in Cisplatin-Induced Experimental Testis Injury Model in Rats

This study was carried out to determine the protective effects of dexpanthenol and boldin against possible testicular damage caused by cisplatin. 48 Male Wistar rats (250-270 g) aged 4-5 months were used in the study. 8 groups were formed with 6 animals in each group. Boldin (20 and 40 mg/kg) and dexpentanol (250 and 500 mg/kg) were administered to rats along with cisplatin (7 mg/kg). At the end of the 14th day, spermatogenic and histopathological examinations and testicular tissue malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) levels were determined. A significant decrease in sperm quality and motility, a significant increase in MDA, and a significant decrease in SOD, GSH-Px, and CAT levels were observed in the cisplatin group. MDA levels were significantly lower in the other groups compared to the cisplatin group. SOD, GSH-Px and CAT enzyme levels increased in the treatment groups compared to the cisplatin group and approached the control group. Combined application of high doses of both substances with cisplatin reduced histopathological lesions. Significant increases were found in the groups in which both doses of dexpanthenol and boldin were combined with cisplatin compared to the cisplatin group. As a result, it was concluded that dexpanthenol and boldin treatments may have a protective effect against cisplatin-induced testicular damage in rats.

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