Ali RİŞVANLI, Meltem KIZIL, Mehmet Akif KILINÇ, Tarık ŞAFAK, Öznur YILMAZ, Burak Fatih YÜKSEL, İbrahim ŞEKER

Luteal Kistli Simental Irkı İneklerde Kan Adipokin, Antioksidan ve Biyokimyasal Parametre Düzeylerinin Belirlenmesi

Bu çalışmada, luteal kistli simental ırkı ineklerde irisin, leptin, adiponektin, asprosin, insülin benzeri büyüme faktörü (IGF-1), bazı biyokimyasal ve antioksidan parametrelerin düzeylerinin belirlenmesi amaçlanmıştır. Bu amaçla 20 adet Simental ırkı inek, luteal kistli (n=10) ve luteal kist bulunmayan (n=10) olmak üzere iki gruba ayrıldı ve ineklerden bir kez kan alınarak ölçümler yapıldı. Ölçülen biyokimyasal parametreler total protein, üre, albümin, laktat dehidrogenaz (LDH), kolesterol, trigliserit, triidothironin (T3), tiroksin (T4) ve glikoz’dur. Ölçülen antioksidan parametreler ise malondialdehit (MDA), glutatyon (GSH), glutatyon proksidaz (GSH-PX) ve katalaz’dır (CAT). Örneklerdeki adipokin ve IGF-1 düzeyleri ticari ELISA kitleriyle belirlendi. Oksidatif stres parametrelerinin düzeyleri spektrofotometrik olarak ve biyokimyasal parametre düzeyleri ise otoanalizör ile ölçüldü. Elde edilen veriler ışığında luteal kistli ineklerde adiponektin (4.93±0.86 ng/mL), IGF-1 (74.70±10.77 ng/mL) ve leptin (2.03±0.20 ng/mL) değerlerinin daha yüksek olduğu, antioksidan parametrelerinin ise gruplar arasında farklı olmadığı tespit edildi. Yine albümin (2.11±0.25 g/dL), kolesterol (99.10±13.79 mg/dl), LDH (1458.50±190.87 U/L), T3 (2.48±0.20 pg/dL), total protein (4.89±0.59 g/dL) ve trigliserid (10.90±2.42 mg/dL) değerlerinin luteal kistli ineklerde daha yüksek olduğu belirlendi. Sonuç olarak; ineklerde luteal kist gelişiminin adipokinlerle ilişkili olabileceği ve bu ilişkinin daha ileri çalışmalarla ortaya konmasının yaralı olacağı kanaatine varıldı.

Determination of Blood Adipokine, Antioxidant and Biochemical Parameter Levelsin Simmental Cows with Luteal Cyst

In this study, it was aimed to determine the levels of irisin, leptin, adiponectin, asprosin, insulin-like growth factor (IGF-1), some biochemical and antioxidant parameters in Simmental cows with luteal cyst. For this purpose, 20 Simmental cows were divided into two groups as those with luteal cysts (n=10) and those without luteal cysts (n=10), and analysis were made by taking blood from the cows. The biochemical parameters analyzed were total protein, urea, albumin, lactate dehydrogenase (LDH), cholesterol, triglyceride, triiodothyronine (T3), thyroxine (T4) and glucose. The antioxidant parameters included malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-PX) and catalase (CAT). Adipokine and IGF-1 levels in the samples were determined with commercial ELISA kits. The levels of oxidative stress parameters were analyzed spectrophotometrically and the levels of biochemical parameters were measured with an autoanalyzer. In the light of the data obtained, adiponectin (4.93±0.86 ng/mL), IGF-1 (74.70±10.77 ng/mL) and leptin (2.03±0.20 ng/mL) in cows with luteal cysts It was determined that the antioxidant values were higher and the antioxidant parameters were not different between the groups. Again, albumin (2.11±0.25 g/dL), cholesterol (99.10±13.79 mg/dL), LDH (1458.50±190.87 U/L), T3 (2.48±0 .20 pg/dL), total protein (4.89±0.59 g/dL) and triglyceride (10.90±2.42 mg/dL) values were found to be higher in cows with luteal cysts. As a result; It was concluded that luteal cyst development in cows may be related to adipokines and it would be useful to reveal this relationship with further studies.

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