Gözde ATİLA USLU, Volkan GELEN, Hamit USLU, Mustafa MAKAV, Yasemen ADALI

Sıçanlarda Melatoninin Kronik Sodyum Nitrit Maruziyetine Karşı Koruyucu Etkileri

Bu çalışmada, gıda katkı maddesi olarak kullanılan sodyum nitritin (NaNO2) neden olduğu karaciğer ve böbrek hasarı üzerine melatoninin (Mel) anti-inflamatuvar etkileri araştırıldı. Çalışma grupları kontrol grubu (C), NaN02 grubu (NaN02) ve melatonin + NaN02 grubu (Mel + NaN02) olarak belirlendi. İlk gruba dimetil sülfoksit (DMSO), iki ve üçüncü gruplara NaNO2 oniki hafta boyunca oral olarak uygulandı. Üçüncü gruba NaNO2 uygulamasından 2 saat önce melatonin uygulandı. Sıçanlara oral yoldan 12 hafta süreyle NaNO2 verilmesi (80 mg/kg/gün) serum aspartate aminotransferaz (AST), alanin aminotransferaz (ALT) (P

Protective Effects of Melatonin against Chronic Sodium Nitrite Exposure in Rats

In this study, anti-inflammatory effects melatonin (Mel) on liver and kidney damage induced by sodium nitrite (NaNO2) used as a food additive were investigated. The study groups were control group (C), NaNO2 group (NaNO2) and melatonin + NaNO2 group (Mel + NaNO2). The first group received dimethyl sulfoxide (DMSO) and the second and third groups received NaNO2 orally for twelve weeks. The third group received melatonin 2 hours before the administration of NaNO2. Administration of NaNO2 (80 mg/kg/day) for 12 weeks orally to rats increased serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) (P

PDF

___

1. Al-Gayyar MMH, Al Youssef A, Sherif IO, Shams MEE, Abbas A. Protective effects of arjunolic acid against cardiac toxicity induced by oral sodium nitrite: Effects on cytokine balance and apoptosis. Life Sci 2014; 111: 18-26.
2. Hustad GO, Cerveny JG, Trenk H, et al. Effect of sodium nitrite and sodium nitrate on botulinal toxin production and nitrosamine formation in wieners. Appl Environ Microbiol 1973; 26: 22-26.
3. Sherif IO, Al-Gayyar MM. Antioxidant, anti-inflammatory and hepatoprotective effects of silymarin on hepatic dysfunction induced by sodium nitrite. Eur Cytokine Netw 2013; 24: 114-121.
4. Al-Gayyar MM, Hassan HM, Alyoussef A, et al. Nigella sativa oil attenuates chronic nephrotoxicity induced by oral sodium nitrite: Effects on tissue fibrosis and apoptosis. Redox Rep 2016; 21: 50-60.
5. Chow CK, Hong CB. Dietary vitamin E and selenium and toxicity of nitrite and nitrate. Toxicology 2002; 180: 195207.
6. Beckman JS, Beckman TW, Chen J, Marshall PA, Freeman BA. Apparent hydroxyl radical production by peroxynitrite: Implications for endothelial injury from nitric oxide and superoxide. Proc Natl Acad Sci 1990; 87: 16201624.
7. Arlt W, Hewison M. Hormones and immune function: Implications of aging. Aging Cell 2004; 3: 209-216.
8. Reppart SM, Weaver DR, Godson C. Melatonin receptors step into the light: Cloning and classification of subtypes. Trends Pharmacol Sci 1996; 17: 100-102.
9. Reiter RJ, Tan DX, Osuna C, Gitto E. Actions of melatonin in the reduction of oxidative stress. J Biomed Sci 2000; 7: 444-458.
10. Reiter RJ, Tan DX, Galano A. Melatonin reduces lipid peroxidation and membrane viscosity. Front Physiol 2014; 5: 377-380.
11. Srinivasan V, Maestroni GJM, Cardinali DP, et al. Melatonin, immune function and aging. Immunity & Ageing 2005; 2: 17.
12. Tekin ME. Sağlık Bilimleri için Örneklerle Bilgisayarda istatistik. 2. Baskı, Konya: Selçuk Üniversitesi Basımevi, 2010.
13. Suresh KP, Chandrashekara S. Sample size estimation and power analysis for clinical research studies. J Hum Reprod Sci 2012; 5: 7. 14. Whitley E, Ball J. Statistics review 4: Sample size calculations. Critical Care 2002; 6: 335. 15. World Health Organization. “Q&A on the carcinogenicity of the consumption of red meat and processed meat”. http:// www.who.int/features/qa/cancer-red-meat/en/ 13.02.2018.
16. Gui G, Meng SS, Li LJ, et al. Sodium nitrite enhanced the potentials of migration and invasion of human hepatocellular carcinoma SMMC-7721 cells through induction of mitophagy. APS 2016; 51: 59-67.
17. Liu Y, Qin Q, Bo A, et al. The effect of sodium nitrite on induction of apoptosis in human gastric adenocarcinoma epithelia (AGS) cells. Pharmacology & Pharmacy 2015; 6: 496-501.
18. Hassan RO, Ali DS. Determination of content levels of nitrogen species (Nitrite, Nitrate, and N-Nitrosamines) in processed meat consumed in Erbil City. Der Pharma Chemica 2010; 2: 31-37.
19. El–Sheikh NM, Khalil FA. L–Arginine and L–glutamine as immunonutrients and modulating agents for oxidative stress and toxicity induced by sodium nitrite in rats. Food Chem Toxicol 2011; 49: 758-762.
20. Tong M, Longato L, De La Monte SM. Early limited nitrosamine exposures exacerbate high fat diet-mediated type 2 diabetes and neurodegeneration. BMC Endocrol Disord 2010; 10: 4.
21. Hassan HA, El-Agmy SM, Gaur RL, et al. In vivo evidence of hepato-and reno-protective effect of garlic oil against sodium nitrite-induced oxidative stress. Int J Biol Sci 2009; 5: 249-255.
22. Elsherbiny NM, Maysarah NM, El-Sherbiny M, Al-Gayyar MM. Renal protective effects of thymoquinone against sodium nitrite-induced chronic toxicity in rats: Impact on inflammation and apoptosis. Life Sci 2017; 180: 1-8.
23. Ohta Y, Kongo M, Sasaki E, Nishida K, Ishiguro I. Therapeutic effect of melatonin on carbon tetrachlorideinduced acute liver injury in rats. J Pineal Res 2000; 28: 119-126.
24. Nava M, Romero F, Quiroz Y, et al. Melatonin attenuates acute renal failure and oxidative stress induced by mercuric chloride in rats. Am J Physiol Renal Physiol 2000; 279: F910-F918.
25. Karaoz E, Gultekin F, Akdogan M, Oncu M, Gokcimen A. Protective role of melatonin and a combination of vitamin C and vitamin E on lung toxicity induced by chlorpyrifosethyl in rats. Exp Toxicol Pathol 2002; 54: 97-108.
26. Othman AI, Al Sharawy S, El-Missiry MA. Role of melatonin in ameliorating lead induced haematotoxicity. Pharmacol Res 2004; 50: 301-307.
27. Ding K, Wang H, Xu J, et al. Melatonin stimulates antioxidant enzymes and reduces oxidative stress in experimental traumatic brain injury: The Nrf2–ARE signaling pathway as a potential mechanism. Free Radical Bio Med 2014; 73: 1-11.