Mete CİHAN, Metin ÖĞÜN, Oğuz MERHAN, Kadir BOZUKLUHAN, Gürbüz GÖKÇE

Omfalitisli Buzağılarda Bazı Oksidatif Stres Parametre Düzeylerinin Belirlenmesi

Bu çalışmanın amacı neonatal dönemde sık görülen göbek yangılarında (omfalitis) oksidatif stres parametre düzeylerinin belirlenmesidir. Bu amaçla çalışmada kontrol grubu olarak 10 adet klinik açıdan sağlıklı buzağı ve 1-2 aylık, 20 adet farklı cinsiyette (16 erkek, 4 dişi) omfalitisli toplam 30 adet montofon ırkı buzağı kullanıldı. Biyokimyasal analizler için kan örnekleri V. jugularis'ten alındı. Alınan kan örneklerinden bir miktarı tam kan olarak ayrıldıktan sonra geriye kalan kanların serumları elde edildi. Tam kan redükte glutatyon (GSH), serum malondialdehit (MDA) ve nitrik oksit (NO) düzeyleri kolorimetrik yöntemlerle belirlendi. Omfalitisli buzağılarda MDA ve NO düzeyleri artarken, GSH düzeyinin azaldığı belirlendi. Sonuç olarak, omfalitisin oksidatif stres ve lipid peroksidasyonuna sebep olduğu belirlendi.

Determination of the Levels of Some Oxidative Stress Parameters in Calves with Omphalitis

The objective of this study was to investigate the oxidative stress parameter levels in navel inflammation (omphalitis) seen frequently during neonatal period. For this purpose, 10 clinically healthy calves as control and 20 calves with omphalitis at 1-2 months of age with different genders (16 males, 4 females) a total of 30 Brown Swiss calves were used. Blood samples were collected from V. jugularis for biochemical analyses. Some part of the blood samples were stored without any process as whole blood. The serum was seperated from remaining blood samples. Whole blood reduced glutathione (GSH), serum malondialdehyde (MDA) and nitric oxide (NO) levels were determined by colorimetric methods. Levels of MDA and NO were determined to increase in blood whereas GSH decreased in calves with omphalitis. In conclusion, the results indicated that omphalitis causes oxidative stress and lipid peroxidation.

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