Nagehan ÇİMENOĞLU, Seval KELEK, Kenan ÇINAR

Kadife balığı (tinca tınca L. 1758) derisinde mukus hücrelerin histokimyasal yapısı

Bu çalışmada Kadife Balığı (Tinca tinca L. 1758)'nın gular, pelvik ve anal yüzgeç arası bölge, pelvik ve pektoral yüzgeç arası bölgelerinden alınan deri mukus hücrelerinin histokimyasal karakterlerinin belirlenmesi amaçlandı. Her üç bölgede de sülfatlı asidik glikokonjugatların güçlü reaksiyon gösterdiği belirlendi.. Uygulanan AB pH 2.5/PAS boyama metodu sonucunda gular bölgede sadece asidik glikokonjugat içeren mukus hücrelerinin bulunduğu, diğer bölgelerde de hem asidik hemde nötral giikokonjugat içeren hücrelerin bulunduğu belirlendi. Güçlü sülfatlı ve O- sülfat esterli glikokonjugatların gular ve pelvik-anal yüzgeç arası bölgedeki mukus hücrelerinde güçlü, pelvik-pektoral yüzgeç arası bölgedeki mukus hücrelerinde zayıf reaksiyon gösterdiği tespit edildi.

The histochemical structure on the mucous cells in skin of the tench (tinca tinca L. 1758)

This study was aimed to determine histochemical characters of mucus cells taken from regions of gular, between pelvic and anal fin region, between pelvic and pectoral fin of the Tench (Tinca tinca L. 1758). It was identified that strong reaction acidic sulfate glycoconjugates showed strong reactions in all three regions. From the AB pH 2.5/PAS painting method, only mucus cells containing acidic glycoconjugate were found, in gular region, the cells containing both acidic glycoconjugate neutral were found in the other regions. Strong sulphated and O-sulfate ester of glycoconjugates showed strongest reactions in the mucous cells in gular and between pelvic and anal fins regions, and weak reactions in the mucous cells in the region between pelvic and pectoral fins.

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1. Zaccone G. Histochemical studies of acid proteoglycans and glycoproteins and activities of hydrolytic and oxidoreductive enzymes in the skin epidermis of the fish Blennius sanguinolentus pallas. Histochem Cell Bio 1983; 78(2): 163-175.
2. Ekingen G. Balık anatomisi. Mersin Üniversitesi Su Ürünleri Fakültesi Yayınları.No: 1, Mersin: Güven, 2001.
3. Sarıhan E, Cengizler İ. Temel balık anatomisi ve fizyolojisi. Çukurova Üniversitesi Su Ürünleri Fakültesi, Adana: Nobel Kitabevi, 2006.
4. Demir N. ihtiyoloji, 4. Baskı, 424, Ankara: Nobel Yayın Dağıtım, 2009.
5. Bat L, Erdem Y, Ustaoğlu Tırıl S, Yardım Ö. Balık Sistematiği 1. Baskı, 26-27, Ankara: Nobel Yayın Dağıtım, 2008.
6. Genten Fy Terwinghe E, Danguy A. Atlas of fish histology. '757, New Hampshire, USA: Enfield, 2009.
7. Zaccone G, Kapoor BG, Fasulo S, Ainis L. Structural, histochemical and functional aspects of the epidermis of fishes. Adv Mar Biol 2001; 40: 253-348.
8. Whitear M. The skin of fishes including cyclostomes In: Bereiter-Hahn J.Matoltsy AG, Richards KS (Editors). Biology of the Integument. Vol. 2 Vertebrates. Berlin: Springer-Verlag, 1986: 8-64.
9. Allen A. Structure and function of gastrointestinal mucus. In: Johnson LR (Editor). Physiology of the gastrointestinal tract. New York: Raven Pres, 1981: 617-639.
10. Shephard KL. Functions for fish mucus. Rev Fish Biol Fish 1994;4:401-29.
11. Fletcher TC, Jones R, Reid L. Identification of glycoproteins in goblet cells of epidermis and gill of plaice (Pieuronectes platessa L.), flounder (Platichthys flesus (L.)) and rainbow trout (Salmo gairdneri Richardson). Histochem J 1976; 8: 597-608.
12. Alan D, Pickering D, Macey DJ. Structure, histochemistry and the effect of handling on the mucous cells of the epidermis of the char Salvelinus alpinus (L.). J Fish Biol 1977; 10(5): 505-512.
13. Gona O. Mucous glycoproteins of teleostean fish: a comparative histochemical study. Histochem J 1979; 11: 709-718.
14. Mittal AK, Whitear M, Agarwal SK. Fine structure and histochemistry of the epidermis of the fish. Monopterus cuchia J Zoo/1980; 191(1): 107-125.
15. Mittal AK, Ueda T, Fujimori O, Yamada K. Histochemical analysis of glycoproteins in the unicellular glands in the epidermis of an Indian fresh water fish Mastacembelus pancalus (Hamilton). Histochem J 1994; 26: 666-677.
16. McManus JFA. Histological and histochemical uses of periodic acid. Stain Technol 1948; 23: 99-108.
17. Lev R, Spicer SS. Specific staining of sulphate groups with alcian blue at low pH. J Histochem Cytochem 1964; 12: 309.
18. Mowry RW. Alcian blue tecniques fort he histochemical study of acidic carbohydrates. J Histochem cytochem 1956; 4: 407-408.
19. Gomari G. Gomari's aldehyde fuchsin stain. In: Culling CFA, Allison RT, Barr WT. (Editors). Cellular Pathology Tecnique. London: Butterworths, 1952: 238.
20. Spicer SS, Mayer DR. Aldehyde fuchsin/Alcian blue. In: Culling CFA, Allison RT, Barr WT. (Editors). Cellular Pathology Tecnique. London: Butterworths, London, 1960: 233.
21. Al-Banaw A, Kenngott R, Al-Hassan JM, Mehana N, Sinowatz F. Histochemical analysis of glycoconjugates in the skin of a catfish (Arius tenuispiriis, Day). Anat Histol Embryol 2009; 39(1): 42-50.
22. Sarasquete C, Gonzalez de Canales ML, Arellano J, et al. Histochemical study of skin and gills of Senegal sole, Solea senegalensis larvae and adults. Histtol Histopathol 1998; 13(3): 727-735.
23. Carmignani MP, Zaccone G. Histochemical analysis of epidermal cells in the skin of Torpedo ocellata Rafinesque. Acta Histochem 1975; 52(1): 100-110.
24. Lopez-Vidriero MT, Jones R, Reid L, Fletcher TC. Analysis5 of skin mucus of plaice Pieuronectes platessa. J Comp Patho 1980; 90(3): 415-420.
25. Harris JE, Watson A, Hunt S. Histochemical analysis of mucous cells in the epidermis of Brown trout Salmo trutta L. Comp Biochem Physiol 1972; 54: 325-328.
26. Özen, MR, Demirbağ E, Çınar K. Kalkan balığı {Psetta maxima) derisinde mukus hücrelerinin dağılımı ve histokimyasal yapısı. 20. Ulusal biyoloji kongresi. Pamukkale Üniversitesi, Denizli, Türkiye, 747-748, 2010.