Emrah Hicazi AKSU, Gaffari TÜRK, Tanzer BOZKURT

Farklı senkronizasyon uygulamaları ile senkronize edilen ineklerde üreme performansı üzerine vitamin E’nin etkis

Bu çalışma; farklı senkronizasyon yöntemleri kullanılarak senkronize edilen ineklerin üreme performansı üzerine vitamin E uygulamasının etkisini araştırmak amacıyla yapıldı. Çalışmada hayvan materyali olarak 84 adet Holştayn ırkı inek kullanıldı. Çift doz prostaglandin $F _{2alpha}$ (11 gün arayla $PGF _{2alpha}$), Ovsynch yöntemi ve Kontrollü intravaginal ilaç salımı (CIDR) yöntemi ile üç farklı şekilde senkronize edilen inekler her senkronizasyon grubu içerisinde kontrol ve vitamin E uygulanan grup olarak iki gruba ayrıldı. Tüm gruplardan 10 ml kan alınarak kontrol gruplarına 4 ml i.m. serum fizyolojik, uygulama gruplarına da 4 ml vitamin E (300 mg/ 2 ml) enjeksiyonu yapıldı. Alınan kan örneklerinde vitamin E ve MDA düzeyleri tespit edildi. Bütün gruplardaki hayvanlar tohumlamadan 60 gün sonra gebelik açısından muayene edildi. Gebelik oranları açısından her bir senkronizasyon grubu içerisindeki kontrol ve uygulama grupları ile senkronizasyon grupları arasında istatistikî olarak önemli bir farklılık tespit edilmedi. CIDR uygulanan ineklerde kontrol (p

Effect of vitamin E on reproductive performance in cows synchronized with different synchronization methods

This study was conducted to investigate the effect of vitamin E treatment on reproductive performance of cows synchronized with different synchronization methods. Eigthy-four holstein cows were used as material. The cows that were synchronized by double $PGF _{2alpha}$ injections 11 days apart, ovsynch and CIDR insertion were divided into two groups as control and treatment (vitamin E) within each synchronization group. Ten ml blood samples were taken from all groups, and then 4 ml physiological saline was injected to all control groups, 4 ml vitamin E (300 mg/ 2 ml) injected to all treatment groups. Vitamin E and MDA levels were determined in all blood samples. All the animals were examined for pregnancy on day 60 after insemination. No statistically significant difference was determined between different synchronization groups, and between control and treatment subgroups within same synchronization group with respect to pregnancy rates. There was a statistically significant difference between MDA levels of first and second blood samples in both control (p

PDF

___

1. Geary TW, Whittier JC. Effect of a timed insemination following synchronization of ovulation using the Ovsynch or Co-Synch protocol in beef cows. Prof Anim Sci 1998; 14: 217-220.
2. Xu ZZ, Burton LJ. Reproductive performance of dairy heifers after estrus synchronization and fixedtimed artificial insemination. J Dairy Sci 1998; 82: 910-917.
3. Foote RH. Estrus detection and estrus detection aids. J Dairy Sci 1975; 58: 248-256
4. Sturman H, Oltenacu EAB, Foote R.H. Importance of inseminating only cows in estrus. Theriogenology 2000; 53: 1657-1668.
5. Williamson NB, Morris R.S, Blood D.C, et al. A study of oestrus behaviour and oestrus detection methods in a large commercial dairy herds. Vet Rec 1972; 91: 58-62.
6. Canooğlu E. İneklerde senkronizasyon amaçlı Prostaglandin F2α uygulamalarından sonra oluşacak östrusların görülme zamanı. Erciyes Üniv Vet Fak Derg 2004; 1: 43-47.
7. Çoyan K, Ataman MB, Erdem H, ve ark. Synchronization of Estrus in Cows Using Double $PGF _{2alpha}$, GnRH- $PGF _{2alpha}$ and HCG- $PGF _{2alpha}$ Combination. Revue de Med Vet 2003; 154: 91-96.
8. Macmillan KL, Henderson H.V. Analyses of the variation in the interval form an injection of prostaglandin $F _{2alpha}$ to oestrus as a method of studying patterns of follicle development during dioestrus in dairy cows. Anim Reprod Sci 1984; 6: 245-254.
9. Milvae RA, Hinckley ST, Carlson JC. Luteotrpic and luteolytic mechanism in bovine corpus luteum. Theriogenology 1996; 45: 1327-1349.
10. Cleef JV, Macmıllan KL, Drost M, Lucy MC, Thatcher WW. Effects of administering progesteron at selected intervals after insemination of synchronized heifers on pregnancy rates and resynchronization of returns to service. Theriogenology 1996; 46: 1117-1130.
11. Çoyan K, Ataman MB, Erdem H, ve ark. Synchronization of Estrus in Cows Using Double PGF2a, GnRH- PGF2a and HCG- PGF2a Combination. Revue de Med Vet 2003;154: 91-96.
12. Olson J. Improving Pregnancy Rates in High Producing Herds, Western Dairy Management Conference, Las Vegas, Nevada. 1999
13. Pursley JR, Mee M.O, Wiltbank M.C. Synchronization of ovulation in dairy cows using PGF2 alpha and GnRH. Theriogenology 1995; 44: 915-923.
14. Thatcher WW, Moreira F, Pancarci M, et al. Strategies to optimize reproductive efficiency by regulation of ovarian function. Dom Animal Endocrinology 2002; 23: 243-254.
15. Cheeseman KH, Slater TF. An Introduction to Free Radical Biochemistry. Br Med Bull 1993;49: 481- 493.
16. Ishikawa M. Oxygen radicals-superoxide dismutase system and reproduction medicine. Nippon Sanka Fujinka Gakkai Zasshi 1993; 45: 842-848.
17. Jozwik M, Wolczynski S, Szamatowicz M. Oxidative stress markers in preovulatory follicular fluid in humans. Mol Hum Reprod 1999; 5: 409-413.
18. Sugino N, Takiguchi S, Kashida S, et al. Superoxide dismutase expression in the human corpus luteum during the menstrual cycle and in early pregnancy. Mol Hum Reprod 2000; 6: 19-25.
19. Suzuki T, Sugino N, Fukaya T, et al. Superoxide dismutase in normal cycling human ovaries: immunohistochemical localization and characterization. Fertil Steril 1999;72: 720-726.
20. Vega M, Johnson M.C, Diaz H.A, et al. Regulation of human luteal steroidogenesis in vitro by nitric oxide. Endocrine 1998;8: 185-191.
21. Aréchiga CF, Vázquez-Flores S, Ortiz O, et al. Efect of injection of β-carotene or Vitamin E and selenium on fertility of lactating dairy cows. Theriogenology 1998;50: 65-76.
22. Fainaru O, Almog B, Pinchuk I, et al. Active labour is associated with increased oxidisibility of serum lipids ex vivo. Br. J Obst Gynecol 2002;109: 938- 941.
23. Wall PD, Pressman EK, Woods J.R. (Preterm premature rupture of the membranes and antioxidants: the free radical connection. J Perinat Med 2000; 30: 447-457.
24. Sönmez M, Bozkurt T, Türk G, ve ark. The effect of vitamin E treatment during preovulatory period on reproductive performance of goats following estrous synchronization using intravaginal sponges. Anim Reprod Sci 2009;114: 183-192.
25. Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 2005; 3: 28.
26. Seino T, Saito H, Kaneko T, et al. Eight-hydroxy-2- deoxyguanosine in granulosa cells is correlated with the quality of oocytes and embryos in an in vitro fertilization-embryo transfer program. Fertil Steril 2002; 77: 1184-1190.
27. Agarwal A, Allamaneni S.S. Role of free radicals in female reproductivediseases and assisted reproduction. Reprod Biomed Online 2004; 9: 338- 347.
28. Pierce JD, Cackler AB, Arnett MG. Why should you care about free radicals? R.N.2004;67: 38-42.
29. Van Langendonckt A, Casanas-Roux F, Donnez J. Oxidative stress and peritoneal endometriosis. Fertil Steril 2002; 77: 861-870.
30. Fujii J, Iuchi Y, Okada F. Fundamental roles of reactive oxygen species and protective mechanisms in the female reproductive system. Reprod Biol Endocrinol 2005; 3: 43.
31. Aréchiga CF, Ortíz O, Hansen PJ. Effect of prepartum injection of vitamin E and selenium on postpartum reproductive function of dairy cattle. Theriogenology 1994; 41: 1251-1258.
32. Sales JNS, Dias LMK, Viveiros ATM, et al. Embryo production and quality of Holstein heifers and cows supplemented with β-carotene and tocopherol. Anim Reprod Sci 2008; 106: 77-89.
33. Okawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues bythiobarbituric acid reaction. Anal Biochem 1979; 95: 351-358.
34. Aksoy M, Alan M, Tekeli T, ve ark. İnek ve düvelerde östrus belirleme hataları ve suni tohumlama uygulamasındaki önemi. Assuit Vet Med J 1992; 53: 284-288.
35. Karaca F, Gülyüz F, Uslu B.A. Saha şartlarındaki sığırlarda ikinci PGF2α enjeksiyonunu takiben östrüste tohumlamalar ile sabit zamanlı tohumlamaların karşılaştırılması. YYÜ Sağ Bil Derg 2006; 9: 226-230.
36. King GJ, Robertson HA. A two injection schedule with prostaglandin F2α for the regulation of the ovulatory cycle of cattle. Theriogenology 1974;1: 123-128.
37. Lokhande SM, Patil VH, Mahajan DC, et al.Fertility on synchronized estrus in crossbred (Bos taurus × Bos indicus) heifers. Theriogenology 1983;20: 397- 406.
38. Xu ZZ, Burton LJ, Macmillan KL. Reproductive performance of lactating dairy cows following estrus synchronization regimens with PGF2α and progesterone. Theriogenology 1997;47: 687-701.
39. Kırbas M, Çoyan K, Bülbül B, ve ark. İnek ve Düvelerde Luteal Aktivitenin Ovsynch Protokolüne Etkisi. Uludag Univ J Fac Vet Med 2008; 27: 47-52.
40. Mialot JP, Constant F, Dezaux P, et al. Estrus synchronization in beef cows: comparison between GnRH + PGF2α + GnRH and PRID + PGF2α + eCG. Theriogenology 2003; 60: 319-330.
41. Lynch GP. Changes of tocopherols in blood serum of cows fed hay or silage. J Dairy Sci. 1983;66: 1461-1465.
42. Sivertsen T, Overnes G, Osterås O, et al. Plasma vitamin E and blood selenium concentrations in Norwegian dairy cows: regional differences and relations to feeding and health. Acta Vet Scand 2005; 46: 177-191.