Benzo(A)piren uygulanan ratlarda E Vitamini ve selenyumun kan ve dokularda lipit peroksidasyonu ve bazı antioksidan enzimler üzerine koruyucu etkileri

Bu çalışmada, benzo(a)piren (BaP) verilen ratlarda E vitamini ve selenyumun kan ve dokularda lipit peroksidasyonu ve bazı antioksidan enzimler üzerine etkileri araştırıldı. Bu amaçla, 100 Wistar Albino türü dişi rattan beş grup oluşturuldu. Bu gruplar; 1. kontrol, 2. BaP, 3. BaP + E vitamini, 4. BaP + selenyum, 5. BaP + E vitamini + selenyum olarak ayrıldı. Çalışma bulgularına göre, BaP’ın plazma ve karaciğer lipit peroksidasyonunu artırdığı, eritrosit ve karaciğerdeki antioksidan enzim düzeylerini azalttığı saptanmıştır. Diğer yandan E vitamini ve selenyum, plazma ve karaciğer MDA düzeylerini azaltırken, eritrosit ve karaciğer GSH, GSH-Px ve katalaz düzeylerini artırmıştır. Sonuç olarak, E vitamini ve selenyumun; BaP’ın sebep olduğu antioksidan enzim düzeyleri ve lipit peroksidasyonu üzerindeki olumsuz etkilerini düzeltmede ve kanserojen etkinliğini önlemede yararlı olduğu belirlenmiş, özellikle her ikisinin birlikte kullanılmasının ise daha koruyucu olduğu gözlenmiştir.

Protective effects of vitamin E and selenium on blood and tissues lipid peroxidation and some antioxidant enzyme levels in rats exposed to benzo(A)pyrene

The effects of vitamin E and selenium on blood and tissues lipid peroxidation and some antioxidant enzymes levels in rats exposed to benzo(a)pyrene (BaP) was investigated. For this purpose, 100 Wistar Albino female rats were divided in 5 groups, as group 1 (Control), group 2 (BaP), group 3 (BaP + vitamin E), group 4 (BaP + selenium), and group 5 (BaP + vitamin E + selenium). The results of the present study showed that administration of BaP caused an increase in the levels of plasma and liver lipid peroxidation and a decrease in the levels of antioxidant enzymes in the erythrocyte and liver. On the other hand, the administration of vitamin E and selenium decreased the levels of plasma and liver MDA, while increasing the levels of erythrocyte and liver GSH, GSH-Px and catalase. As a result, vitamin E and selenium administrations were useful in preventing the negative effects of BaP on the lipid peroxidation, antioxidant enzymes and its carcinogenic effects. Furthermore, vitamin E combined with selenium was more effective.

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1. Rames A, Inyoung F, Hood DB, et al. Methabolism, bioavailability, and toxicokinetics of benzo(a)pyrene in F- 344 rats following oral administration. Exp Toxicol Pathol 2001; 53: 275-290.
2. Seven A, Candan G. Antioksidan savunma sistemleri. Cerrahpaşa Med Rev 1996; 27: 41-50.
3. Badary OA, AbdEl-Gavad HM, Taha RA, Ali AA, Hamada FMA. Effects of benzo(a)pyrene on tissue activities of methabolising enzymes and antioxidant systeme in normal and protein-malnourished rats. J Biochem Mol Toxicol 2003; 17 (2): 86-91.
4. Evangelou A, Kalpouzos G, Karkabounas S, Liasko R, Nonni A, Stefanou D, Kallistratos G. Dose-related preventive and therapeutic effects of antioxidantsanticarcinogens on experimentally induced malignant tumors in Wistar rats. Cancer Lett 1997; 115: 105-111.
5. Lee BM, Lee SK, Kim HS. Inhibition of oxidative DNA damage, 8-OhdG, and carbonyl contents in smokers treated with antioxidants (vitamin E, vitamin C, β-carotene and red ginseng). Cancer Lett 1998; 132: 219-227
6. Das UN. Effect of anti-oxidants, free radical quenchers and siklo oxigenase inhibitor on benzo(a)pyrene-induced suppression of human lymphocyte mitogenesis in vitro. Med Sci Monit 2002; 8 (6): 205-207.
7. Lowy DF, Base R, Simo AA. Evidence for a high free radical state in low-grade astrocytomas. Neurosurgery 1997; 41: 146-150.
8. Boutin A.C., Shirali P., Garçon G. Gosset P at al.. Peripheral markers (clara cell protein and α-glutathione STransferase) and lipidperoxidation (Malondialdehyde) assesment in sprague-dawley rats ınstilled with haematite and benzo(a)pyrene. J App Toxicol 1998; 18(1): 39-45
9. David W, Martin JR, Peter AM, Victor WR, Daryl K G. Harper’s Review of Biochemistry. Twentieth Edition, 1988.
10. Dündar Y, Aslan R. Bir antioksidan olarak vitamin E, Genel Tıp Derg 1999; 3(9): 109-116.
11. Morrissey PA, O’Brien NM. Dietary antioxidants in health and disease. Int Dairy J 1988; (8): 463-472.
12. Ricciarelli R, Zingg JM, Azzi A. Vitamin E: protective role of a Janus molecule. Faseb J 2001; 15: 2314-2325.
13. Ulrey DE. Vitamine E for swine. J Anim Sci 1981; 53(4): 1039-1055.
14. Valko M, Rhodes CJ, Moncol J, Izakovıc M, Mazur M. Free radicals, metals and antioxidants in oxidative stres-ınduced cancer. Chem-Biol Interact 2006; 160: 1-40.
15. Putnam ME, Comben N. Vitamin E (review article). Vet Rec 1987; 5: 541-545.
16. Combs GF and Combs SB. The Role of Selenium in Nutrition. London: Academic press. Inc. Ltd. 1986.
17. Raymond JS. Selenium metabolism and function. J Anim Sci 1986; 4: 42-49.
18. Sies H, Stahl W and Sundquıst AR. Antioxidant Functions of Vitamins. Annals New York Academy of Sciences, 1982; 669: 7-15.
19. Placer AZ, Lında LC, Johnson B. Estamination of Product of Lipid Peroxidation (Malonly Dialdehyde) in Biochemical Systems. Anal Biochem 1966; 16: 359-364.
20. Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 1976; 71 (4): 952-958.
21. Sedlak J, Lındsay RHC. Estimation of Total Protein Bound And Nonprotein Sulfhydryl Groups in Tissue With Ellmann’s Reagent. Anal Biochem 1968; 25: 192-205.
22. Goth L. A simple method for determination of serum catalase activity and revision of reference range. Clin Chim Acta 1991; 196: 143-152
23. Aebi H. Catalase. In: Bergmeyer U (Editor). Methods of enzymatic analysis. New York and London: Academic Press, 1974.
24. Gsandhya D, Prasad MH and et al. Free radicals antioxidant enzymes and lipid peroxidation in different types of leukemias. Clin Chim Acta 2000; 93: 53-62.
25. Huang Y, Sheu J, Lin T. Association between oxidative stress and chenges of trace elements in patients with breast cancer. Clin Biochem 1999; 32(2): 131-136.
26. Jaund AF. Role of free radicals in cancer development. Eur J Cancer 1996; 32: 30-38.
27. Kehrer JP., Smith CV. Free radicals in biology: sources, reactivities and roles in the etiology of human disease. In: FREI B. (Ed). Natural antioxidants in human health and disease. San Diego: Academic Pres, 1994
28. Knight JA. Disease related to oxygen-derived free radicals. Ann Clin Lab Sci 1995; 25: 111-121.
29. Nikiforova NV, Khodyreva LA, Kipatovskii V I and et. al. Lipid peroxidation malignant tumors of human kidneys. Bulletin of Experiment Biol Med 2001; 132 (5): 1096-1099.
30. Lee BM, Lee SK, Kim HS. Inhibition of oxidative DNA damage, 8-OhdG, and carbonyl contents in smokers treated with antioxidants (vitamin E, vitamin C, β-carotene and red ginseng). Cancer Lett 1998; 132: 219-227
31. Selvendiran K, Singh JPV, Krishnan KB, Sakthisekaran D. Cytoprotective effect of piperine againist benzo(a)pyrene induced lung cancer with reference to lipid peroxidation and antioxidant system in swiss albino mice. Fitoterapia 2003; 74: 109-115.
32. Yarıktaş M, Döner F, Doğru H, Aynalı G, Yönden Z, Delibaş N. Baş boyun malign tümörlerinde malondialdehid düzeyleri ve antioxidan enzim aktiviteleri. SDÜ Tıp Fak Derg 2003; 10(4): 65-67.