Ahmet ATEŞŞAHİN, İzzet KARAHAN, Seval YILMAZ

Ratlarda cisplatin ve gentamisinin kan ile karaciğerde oluşturdukları oksidatif stres üzerine likopenin etkileri

Bu çalışma, cisplatin ve gentamisinin kan ile karaciğerde oluşturabilecekleri oksidatif stresi belirlemek ve likopenin iyileştirici bir etkisinin olup olmadığını araştırmak amacıyla yapıldı. Araştırmada kullanılan ratlar her grupta 6 hayvan olacak şekilde 5 gruba bölündü ve birinci grup kontrol olarak ayrıldı. Grup 2, 3, 4 ve 5'teki hayvanlara sırasıyla tek doz cisplatin (7 mg/kg); cisplatin uygulamasını takiben 5 gün süreyle likopen (4 mg/kg); 6 gün süre ile gentamisin (100 mg/kg) ve gentamisin ile birlikte likopen uygulamaları yapıldı. Tek doz cisplatin uygulamasını takiben 5. günde, diğer gruplarda ise son uygulamadan 24 saat sonra kan ve karaciğer örnekleri alındı. Bu örneklerde malondialdehid (MDA) ve indirgenmiş glutatyon (GSH) düzeyleri spektrofotometrik yöntemlerle belirlendi. Tek doz cisplatin uygulanan ratlarda plazma MDA ve kan GSH ile karaciğer MDA düzeylerinde önemli artışların olduğu, buna karşın cisplatin uygulamasını takiben likopen verilen grupta bu değerlerin kontrol değerlerine yaklaştığı belirlendi. Karaciğer GSH düzeylerinde ise kontrol grubuna göre bir farklılık olmadığı görüldü. Gentamisin uygulaması yapılan ratlarda ise yalnızca plazma MDA düzeylerinde kontrol grubuna göre bir artış belirlenirken, karaciğer MDA ve GSH ile kan GSH düzeylerinin değişmediği tespit edildi. Diğer yandan, likopenle birlikte gentamisin uygulanan grupta ise plazma MDA düzeylerinin kontrol değerlerine yaklaştığı belirlendi. Sonuç olarak, ratlarda gentamisin uygulamasının kan ve karaciğerde oksidatif stres oluşumunu önemli ölçüde etkilemediği, buna karşın cisplatin uygulamasının ise oksidatif stresi artırdığı ve likopen uygulamasının ise artan oksidatif stresi engelleyebileceği kanaatine varıldı.

Effects of lycopene on cisplatin and gentamicin induced oxidative stres in blood and liver in rats

This study was carried out to establish the effects of lycopene on administrations of high dose cisplatin and gentamicin induced oxidative stres in blood and liver and to investigate whether lycopene have any ameloriate effects. Rats were divided into 5 groups, each consisting of 6 rats. Group 1 used as control. Animals of group 2, 3, 4 and 5 were administrated single dose cisplatin (7 mg/kg); cisplatin following 5 days lycopene (4 mg/kg); gentamicin for 6 days (100 mg/kg) and gentamicin together with lycopene respectively. Blood and liver tissue samples were collected 5 days after single dose cisplatin and 24 hours after the last treatment in other groups. The levels of malondialdehyde and reduced glutathione levels were determined spectrophotometrically. Significant increases in plasma MDA, blood GSH and liver MDA, levels were observed in single dose cisplatin treated rats. Administration of lycopene after cisplatin produced decreases in these parameters. No change was observed liver GSH levels in these groups compared to the control group. While significantly increase was determined alone the levels of plasma MDA 6 days treatment of gentamicin in group 4 when compared control group; liver MDA, GSH and blood GSH levels were not changed. Normalization of plasma MDA levels was determined in treated lycopene together with gentamicin group. In conclusion, treatment with gentamicine were not affected on oxidative stres in blood and liver, but cisplatin increased in rats, and it was concluded that lycopene may prevent increasing oxidative stres in blood and liver.

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