Klamidiozisli insanlarda serbest radikal hasarı ile geçiş metallerinden Fe ve Cu arasındaki ilişkiler

Zorunlu hücre içi patojenleri olan klamidialar patojenik mekanizmaları tam olarak bilinmeyen pek çok hastalığa sebep olurlar. Bir veya daha fazla ortaklanmamış elektron içeren atom veya moleküller olan serbest radikaller aktive olmuş fagositlerin bakterisidal rollerinin sonucunda üretilirler. Hücre bütünlüğünü tehdit eden en önemli sorunlardan birisi lipid peroksidasyondur ve eser elementlerin lipid peroksidasyon üzerine önemli etkileri vardır. Hidrojen peroksitin demir ve bakır gibi geçiş elementlerinin varlığında indirgenmesi (Fenton reaksiyonu) veya süperoksit radikali ile reaksiyona girmesi sonucunda (Haber-Weiss reaksiyonu) hidroksil radikali oluşur. Hidroksil radikali lipid peroksidasyonu tetikleyici faktördür. Bu çalışmada, klamidia enfeksiyonu sırasında eser element düzeylerini ve bu eser element düzeylerindeki değişimlerin lipid peroksidasyon ile ilişkisini incelemeyi amaçladık. Çalışmada, komplement fıkzasyon testi ile klamidia etkenleri saptanan 15 hasta deneme grubunu oluştururken, 15 sağlıklı birey de kontrol grubu olarak kullanılmış ve alınan kan örneklerinden serumlarda lipid peroksidasyon göstergesi olarak malondialdehid, geçiş periyodu metallerinden demir ve bakır düzeyleri ölçülmüştür. Kontrollerle karşılaştırıldığı zaman enfekte insanların kan serumlarında lipid peroksit (pO.001) ve bakır (p0.05) düzeylerinde ise herhangi bir değişiklik saptanamamıştır. Sonuç olarak klamidial enfeksiyon sırasında reaktif oksijen türlerinin artışına bağlı olarak lipid peroksidasyon düzeyinde artış meydana gelmiştir. Lipid peroksidasyondaki artışa serum bakır düzeyindeki artış eşlik etmiştir. Serum demir konsantrasyonunda ise bir değişiklik gözlenmemiştir. Radikal toksikasyonunu engelleyen süperoksit dismutaz enziminin oksidan olaylar sırasında artması, enzim yıkımındaki artışı da beraberinde getirmektedir. Serumdaki lipid peroksidasyon artışına bakırın eşlik etmesi bakır kaynaklı bu enzimin (Cu-SOD) yıkım ürünü olarak açıklanabilir.

The association between free radical damage and transition metals Cu and Fe in humans with chlamydiosis

Chlamydiae are obligatory intracellular pathogens that cause many diseases of which the pathogenic mechanism are largely unknown. A free radical is defined as an atom or a molecule that has one or more unpaired electrons in one of its molecular orbital and they are produced by activated phagocytes as their bactericidal function. Lipid peroxidation is a significant threatining factor for cellular integrity and trace elements have significant effects on lipid peroxidation. Hydrogen peroxide is reduced by transition elements such as copper and iron (Fenton reaction) or by reacting with superoxide radicals (Haber-Weiss reaction) and thus hydroxil radicals are produced. Hydroxil radicals trigger lipid peroxidation. This study was carried out to determine lipid peroxidation, the level of copper and iron and its association with lipid peroxidation in humans with chlamydiosis. In this study, sera samples of 15 healthy subjects and 15 subjects found to be positive to chlamydia by CFT were used. Malondialdehyde was measured as indicator of lipid peroxidation, and levels of iron and copper were measured as transition metals. When they were compared with the controls, it was observed that levels of lipid peroxid (p

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