RATALARDA FARKLI BİR VAZEKTOMİ TEKNİĞİ

Bu çalışmada erkek ratları kısırlaştırmak amacıyla yeni bir vazektomi tekniği uygulandı. Materyal olarak, 6 aylık fertilitesi ispatlanmış 20 albino rat kullanıldı. Hayvanlar rastgele iki gruba ayrılarak I. gruptaki (n=10) ratlara, ductus deferenslere ligatür konarak vazektomi yapıldı. İkinci gruptaki (n=10) ratlarda ise ductus deferens transversal olarak kesilerek, serbest kalan uçları ayrı ayrı kendi üzerlerine düğümlendi. Operasyon-dan 15 gün sonra iki gruptaki tüm ratların kafeslerine 5 adet dişi rat konularak çiftleşmeleri gözlendi. Sonuç olarak, her iki gruptaki ratlarda post operatif herhangi bir komplikasyona rastlanmadı. Ayrıca, tüm hayvanların dişilerle çiftleştiği görüldü. Ancak yeni operasyon tekniğinin daha kolay, daha ucuz ve daha kısa sürede bittiği tespit edildi.

Anahtar Kelimeler:

Rat, Vazektomi

A DIFFERENT VASECTOMY TECHNIQUE IN RATS

In this work a novel vasectomy technique in rats is presented. A total of 20 albino rats with proven fertility capacity that were 6 months-old were used for this study. They were randomly divided into two groups. In group 1 (n=10), vasectomy was performed by simple placement of ligatures on ductus deferens, whereas in the second group (n=10) ductus deferens was transected transversally and then free edges were separately knotted over itself. Fifteen days after the operation, 5 female rats were placed on cages in order to allow copulation. No complications were encountered postoperatively in either group. All animals achieved copulation. However, we concluded that the new operative technique (the latter) was easier to perform, cheaper and less time consuming.

Keywords:

Rat, Vasectomy,

PDF

___

1. Antrash, L. 1988: Men and Their Worries About Vasectomy: Will a New Surgical Technique Help? AVSC News Association for Voluntary Surgical Contraception. 26: 5-6.
2. Barratt, C.L., Cohen, J. 1988: Quantitative effects of short and long-termvasectomy on mouse spermatogenesis and sperm transport. Contraception, 37 (4): 415-424.
3. Clenney, T.L. and Higgins, J.C. 1999: Vasectomy Techniques. Am. Fam. Physician. 60(1), 137.
4. Davis, L.E., Stockton, M.D. 1997: No-Scalpel Vasectomy. Primary Care. 24 (2), 433-461.
5. Denniston, G.C. 1985: Vasectomy by Electrocautry: Outcomes in a Series of 2.500 Patients. J. Fam. Pract. 21 (1), 35-40.
6. Denniston, G.C. and Kuehl, L. 1994:. Open-Ended Vasectomy: Approaching the İdeal Technique. J. Am. Board Fam. Pract. 7, 285-287.
7. Elaine, A.L. 1991: Frontiers in Hormonal Male Contraception: a Call for Research. Male Contraception Information Project (MCIP) Updated Version- ©3/8/94 First Version ©8/16/1991. MCIP is a Project of the Boston Women's Health Book Collective.
8. Errey, B.B. and Edwards, I.S. 1986: Open-Ended Vasectomy: An Assessment. Fertil Steril. 45, 843-846.
9. Flickinger, C.J., Herr, J.C., Baran, M.L., Howards, S.S. 1995: Testicular development and the formation of spermatic granulosa of the epididymis after obstruction of the vas deferens in immature rats.
10. Grubb, G. S. 1991: Experimental Methods of Contraception. Current Opinion in Obstetric. and Gynecology. 3: 491-495.
11. Hogan, B., Costantini, F. and Lacy, E. 1986: Manipulating the Mouse Embryo, a Laboratory Manual, Cold Spring Harbor Laboratuvary, Cold Spring Harbor.
12. Huber, D. 1989: No-Scalpel Vasectomy: The Transfer of a Refined Surgical Technique From China to Other Countries. Advences in Contraception. (5), 217-218.
13. Kuwahara, M. 1976: The effects of ligation of vas deferens, corpus epididymidis and vasa efferentia on the testicular function in rats. Tohoku J. Exp. Med. 120 (3): 251-257..
14. Reynolds, R.D. 1991: Vas Deferens Occlusion During No-Scalpel Vasectomy. Primary Care, 39 (6), 577-582.
15. Schmidt, S.S. and Minckler, T.M. 1992: The Vas After Vasectomy: Comparison of Cauterization Techniques. Urology. (40), 468-470.
16. Skriver, M., Skovsgaard, F. and Miskowiak, J. 1997: Conventional or Li Vasectomy: a Questionnaire Study. Br. J. Urol. (79), 596-598.
17. Stockton, D.M., Davis, L.E. and Bolton, K.M. 1992: No-Scalpel Vasectomy: A Technique for Family Physician . Am. Fam. Physician. 46, 1153-1167.
18. Waynforth, H.B. and Flecknell, P.A. 1992: Experimental and Surgical Technique in the Rat, Second Ed., Academic Press.
19. Whyte, J., Sarrat, A., Torres, A., Diaz, P., Ortiz, P.P., Cisneros, A., Whyte, A. and Mazo, R. 1998: Experimental Vasectomy: Comparison of the Testicular Structure with Various Surgical Techniques. Actas Urologicals Espanolas, 2 2(3): 178-183.
20. Whyte, J., Sarrat, R., Ortiz, P.P., Mazo, R., Whyte, A., Torres, A. and Cisneros, A.A. 1998: A Morphological and Morphometric Study of the Testicle in the rat Vasectomized by Electrocautry Technicus. Archivos Espanoles de Urologia., 51 (9): 849-855.