Remzi ATILGAN, ŞEHMUS PALA, Tuncay KULOĞLU, Ömer Faruk DOĞAN, Behzat CAN, Murat ATILGAN

The Effect of Administration of Oral Clarithromycin and Tetracycline on Postoperative Adhesion Formation in Rat Uterin Horn Model

Amaç: Oral klaritromisin ve tetrasiklinin postoperatif adezyon oluĢumunu önlemedeki etkisinin incelenmesi. Gereç ve yöntem: 28 adet Wistar-albino cinsi rat rastgele dört gruba ayrıldı. Grup 1 (G1): Batın açılıp kapatılan grup. Grup 2 (G2): Sağ uterin horna 2 cm insizyon yapılıp kapatılan grup. Grup 3 (G3): Oral klaritromisin verildikten 15 dakika sonra sağ uterin horna 2 cm lineer insizyon yapılıp kapatılan grup. Grup 4 (G4): Oral tetrasiklin verildikten 15 dakika sonra sağ uterin horna 2 cm lineer insizyon yapılıp kapatılan grup. 15 gün sonra tüm grupların batınları tekrar açıldı. Sağ uterin horndaki adezyon makromorfolojik karekteristikler ve doku kesitlerinde fibrosis, anjiogenezis, VEGF ve MDA skorlaması temel alınarak değerlendirildi. Ġstatistiksel değerlendirme için Kruskal-Wallis varyans analizi ve Mann-Whitney U testi kullanıldı. Bulgular: Adezyonun Ģiddeti ve yaygınlığı G1, G3 ve G4 te G2 ile karĢılaĢtırıldığında anlamlı derecede daha düĢük idi. Fibrozis ve anjiogenezis skorları G2 de G1, G3 ve G4 ten anlamlı derecede daha yüksek idi. Sonuç: Oral klaritromisin ve tetrasiklin postoperatif adezyonu önlemede etkili bulundu.

Klaritromisin ve Tetrasiklinin Oral Verilmesinin Rat Uterin Horn Modelinde Postoperatif Adezyon Oluşumuna Etkisi

Objective: To investigate effectiveness of oral clarythromycin and tetracycline on the prevention of postoperative adhesions. Materials and Methods: A total of 28 Wistar-albino rats were divided into four groups. Group 1: abdomen was opened and closed, Group 2: a 2linear incision was made on the right uterine horn and closed, Group 3: 15 minutes after the administration of oral clarithromycin, a 2 cm incision was made on the right uterine horn and closed, Group 4: 15 minutes after the administration oral tetracycline, a 2 cm incision was made on the right uterine horn and closed. Fifteen days later, a re-laparotomy was performed in all groups. Right uterine horn adhesions were assessed on the basismacro-morphological characteristics and tissue cross-sections were further examined for the signs of fibrosis, angiogenesis, and VEGF and for MDA scoring. Kruskal-Wallis variance analysis and Mann-Whitney U test were used for statistical analyses. Results: The extent and strength of adhesions were significantly lower in Group 1, Group 3 and Group 4 as compared to Group 2. Fibrosis and angiogenesis scores were significantly higher in Group 2 than in Group 1, Group 3 and Group 4. Conclusion: Oral administration of clarithromycin and tetracycline was effective in the prevention of postoperative adhesions. cm of

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