Streptozotosin ile Oluşturulmuş Diyabetik Sıçanların Beyin Dokusunda İrisin Üzerine Enalaprilin Etkileri

Amaç: Diabetes Mellitus'un (DM) uzun dönem etkileri sonucu; çeşitli hasarlar, disfonksiyonlar, organ yetmezlikleri, özellikle göz, böbrek, kalp ve damar hasarları oluşabilir. İrisin, nörodejenerasyonda oldukça etkili bir protein olan eşleşme bozucu proteinler (UCP)'in gen ekspresyonunu ve mitokondriyal yoğunluğunu artırmaktadır. Bu çalışmada streptozotosin (STZ) ile deneysel diyabet oluşturulan sıçan beyin dokusunda irisin immünreaktivitesi üzerine enalaprilin (EN) etkilerinin incelenmesi amaçlanmıştır. Gereç ve Yöntem: Çalışmamızda 18 adet Wistar albino cinsi erişkin erkek sıçan kullanıldı. Deney hayvanları her grupta 6 sıçan olmak üzere 3 eşit gruba ayrıldı. Kontrol grubuna deney süresi olan 6 hafta süresince herhangi bir işlem yapılmadı. DM grubuna 50 mg/kg tek doz STZ i.p olarak verildi. DM+EN grubuna ise 50 mg/kg tek doz STZ i.p olarak verilip enalapril 5 mg/kg/gün dozunda oral olarak uygulandı. Deney sonunda anestezi altında dekapite edilen sıçanların beyin dokuları hızla çıkartıldı. Beyin dokuları histolojik takip sonrası parafin bloklara gömüldü. Parafin bloklardan alınan kesitlere irisin immünreaktivitesi için avidin-biotin-peroksidaz metodu uygulandı. İmmünohistokimyasal boyanmanın değerlendirilmesinde; immünreaktivitenin yaygınlığı ve şiddeti esas alınarak histoskor oluşturuldu. Bulgular: Kontrol grubu ile karşılaştırıldığında irisin immünreaktivitesi DM grubunda istatistiksel olarak anlamlı bir şekilde azalmıştı. DM grubu ile karşılaştırıldığında ise irisin immünreaktivitesi DM+ EN grubunda anlamlı olarak artmıştı. Sonuç: Diyabetik beyin hasarına karşı efektif olduğu bilinen EN' in birçok mekanizma ile birlikte irisin üzerinden de etki edebileceği kanaatine varılmıştır.

Effects of Enalapril on Irisin Immunoreactivity in the Brain Tissue of Streptozotocin-Induced Diabetic Rats

Objective: Various defects, dysfunctions and organ failures may occur due to long term effects of diabetes mellitus (DM) especially in eye, kidney, heart and vascular system. Irisin increases expression and mitochondrial density of uncoupling proteins (UCPs) which are very important in the neurodegenerative process. In this study we aimed to investigate the effects of enalapril (EN) on irisin immunoreactivity in the brain tissue of streptozotocin (STZ)-induced diabetic rats. Material and method: In this study, 18 adult Wistar albino male rats were used. The study group was divided into 3 equal groups each containing 6 rats. The control group wasn't exposed to any kind of drugs during the 6 weeks of study period. 50 mg/kg single dose STZ was injected to DM group intraperitoneally. DM+EN group rats were given single 50 mg/kg intraperitoneal injection of STZ, oral 5 mg/kg/day enalapril for 6 weeks. Brain tissues of the rats were removed just after the decapitation under the anesthesia. Tissues were embedded into paraffin blocks after the routine histological preparations. Avidin-biotin-peroxidasetechnique was used in determination of irisin expression. Histoscore was created based on the diffusiveness and severity of immunoreactivity in assessment of immunohistochemical staining. Results: There was a significant decrease of irisin immunoreactivity in the DM group. Irisin immunoreactivity was found as significantly increased in DM+EN group compared to DM group. Conclusion: We can conclude that EN shows its effects due to its relationship with irisin along with other various mechanisms.

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