Polyclonal Antibody Production Against HaptenStructured KDN Molecule by Using Different Adjuvants Alternative to Freund’s Adjuvant
Polyclonal Antibody Production Against HaptenStructured KDN Molecule by Using Different Adjuvants Alternative to Freund’s Adjuvant
Objective: KDN (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid), a member of the sialic acid family, is a hapten-structuredlow-molecular-weight monosaccharide on the cell membrane, which cannot induce immune responses without a carrier protein.Since it is over-expressed on the cancerous cells’ membrane, it is thought to be a great target molecule for anti-cancer treatments.The aim of this study is to obtain high titersof the anti-KDN polyclonal antibody response without using any carrier protein againstthe hapten-structured KDN molecule alternative to Freund’s adjuvants.Methods: Montanide™ ISA 61 VG, a water-in-oil adjuvant; ISA 201 VG, a water-in-oil-in-water emulsion adjuvant; and IMS 1313VG NPR, an aqueous-dispersion-based nanoparticle (50-200 nm) microemulsion adjuvant; and Freund’s adjuvant were used asanti-KDN antibody response stimulators. FourBALB/c mice were used for each adjuvant group, and immunization was performedat eight different time points. Anti-KDN antibody levels induced after each immunization with different adjuvants were detectedwith indirect enzyme-linked immunosorbent assay.Results: The adjuvant efficiency of Montanide™ ISA 61 VG water in oil adjuvant was 1.4 times higher than in Freund’s adjuvant(p
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