Ayşe DEMİRKAZIK, Olca KILINÇ, Gökhan ARSLAN, Aykut PELİT, Yavuz TÜRKAY, Ahmet Şevki TAŞKIRAN, Ercan ÖZDEMİR

Comparison of Analgesic Effects Induced by Different Strengths of Extremely Low-Frequency Electromagnetic Fields

Objective: Our aim was to compare the results of the most commonly used analgesic measurement techniques and to determinethe time and intensity at which the analgesic effects of the magnetic field (MF) are most effective.Methods: This study compared the analgesic effect of MF strengths (1, 5, and 10 mT) in 30 adults, male Wistar albino rats weighing 200-250 g. The analgesic effects were measured using tail-flick (TF) and hot-plate (HP) tests. To determine the optimum MFstrength, rats were assigned into four groups: sham group and exposed to 1, 5, and 10 mT MF groups. Rats were placed in a solenoid, and MF of 50 Hz for 165 min was administered daily for 15 days. All four groups were kept in the solenoid for 165 min/15 daysand exposed to MF. However, the analgesic effect was measured only on day 0, 4, 7, 11, and 15 using TF and HP tests. The latenciesof analgesia were converted to a percentage of maximal antinociceptive effects (% MPE).Results: When the maximum analgesic effect of the 5 mT MF was determined on the seventh day, the% MPEs were 5.37±0.51,13.66±1.27, 25.89±3.00, and 25.37±2.41 in the sham, 1 mT, 5 mT, and 10 mT groups, respectively. The optimum effect was observedwith 5 mT MF on the seventh day and with 90 min in the solenoid.Conclusion: We didn’t find any differences between the analgesic responses to the TF and HP tests.

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Sartucci F, Bonfiglio L, Del SeppiaC, Luschi P, Ghione S, Murri L, et al. Changes in pain perception and pain-related somatosensory evoked potentials in humans produced by exposure to oscillating magnetic fields. Brain Res 1997; 769: 362-6. [CrossRef]
Persinger MA, Ossenkopp KP, Glavin GB. Behavioral changes in adult rats exposed to ELF magnetic fields. Int J Biometeorol 1972; 16, 155- 62. [CrossRef]
Houpt TA, Carella L, Gonzalez D, Janowitz I, Mueller A, Mueller K, et al. Behavioral effects on rats of motion within a high static magnetic field Physiol Behav 2011, 102: 338-46. [CrossRef]
Merskey DM, Bogduk N, editors. Classification of chronic pain. Pain Supplement. 1983; 3: S217.
Del Seppia C, Ghione S, Luschi P, Ossenkopp KP, Cholerisd E, Kavaliers M Pain perception and electromagnetic fields.Neurosci Biobehav Rev 2007, 31: 619-42. [CrossRef]
Kosar MI, Demir T, Demirkazik A, Deveci K, Ozdemir E, Gulturk S. Antinociceptive Effects in Normal and Diabetic Rats Exposed to 50 Hz Magnetic Field. Neurophysiology 2012; 44: 56-62. [CrossRef]
Kavaliers M, Ossenkopp KP, Lipa SM, Day-night rhythms in the inhibitory effects of 60 Hz magnetic fields on opiate-mediated ‘analgesic’ behaviors of the land snail, Cepaea nemoralis. Brain Res1990; 517: 276-82. [CrossRef]
Thomas AW, Kavaliers M, Prato FS, Ossenkopp KP. Antinociceptive effects of a pulsed magnetic field in the land snail, Cepaea nemoralis. Neurosci Lett 1997; 222: 107-10. [CrossRef]
Calcutt N., Potential mechanisms of neuropathic pain in diabetes. Int Rev Neurobiol 2002, 50, 205-28. [CrossRef]
Rishi M. Rakesh & Rashmi Mathur. 2008, Threshold of Pain in Chronic Magnetic Field- (50 Hz, 17.9 µT) Exposed Rats: Effect of Sucrose Ingestion.Electromagn Biol Med 2008; 27: 254-65. [CrossRef]
Kanaan SA, Saadé NE, Haddad JJ, Abdelnoor AM, Atweh SF, Jabbur SJ, et al. Endotoxin-induced local infl ammation and hyperalgesia in rats and mice: a new model for infl ammatory pain. Pain 1996, 66: 373-9. [CrossRef]
Luo FL, Yang N, He C, Li HL, Li C, Chen F. Exposure to extremely low-frequency electromagnetic fields alters the calcium dynamics of cultured entorhinal cortex neurons. Environ Res 2014; 135: 236- 46. [CrossRef]
Wolsko PM, Eisenberg DM, Simon LS, Davis RB, Walleczek J, MayoSmith M, et al. Double-blind placebo-controlled trial of static magnets for the treatment of osteoarthritis of the knee: results of a pilot study. Altern Ther Health Med 2004; 10, 36-43.
Prato FS, Robertson JA, Desjardins D, Hensel J, Thomas AW. Daily repeated magnetic field shielding induces analgesia in CD-1 mice. Bioelectromagnetics 2005; 26: 109-17. [CrossRef]
Kavaliers M, Ossenkopp KP. Repeated naloxone treatments and exposure to weak 60 Hz magnetic field have analgesic effects in snails. Brain Research 1993; 620: 159-62. [CrossRef]
Tiffany ST, Maude-Griffin PM. Tolerance to morphine in the rat:Associative and nonassociative effects. Behav Neurosci 1988; 102: 534- 43. [CrossRef]
Zecca L, Mantegazza C, Margonato V, Cerretelli P, Caniatti M, Piva F, et al. Biological effects of prolonged exposure to ELF electromag netic fields in rats, III: 50 Hz electromagnetic fields. Bioelectromagnetics 1998; 19: 57-66. [CrossRef]
Bao X, Shi Y, Huo X, Song T. A possible involvement of beta-endorphin, substance P, and serotonin in rat analgesia induced by extremely low frequency magnetic field. Bioelectromagnetics. 2006; 27: 467-72. [CrossRef]
Langerman L, Zakowski MI, Piskoun B, Grant GJ. Hot Plate Versus Tail Flick: Evaluation of Acute Tolerance to Continuous Morphine Infusion in the Rat Model J PharmacolToxicol Methods 1995; 34: 23-7.
Robertson JA, Juen N, Théberge, Weller JJ, Drost DJ, Prato FS, et al. Evidence for a dose-dependent effect of pulsed magnetic fields on pain processing. Neurosci Lett 2010; 482: 160-2. [CrossRef]