Özgür İlke ATASOY ULUSOY, Güliz GORGUL, Doruk ENGİN

Primer kok kanal enfeksiyonlarında Tannerella forsythia prevalansının 16S rRNA PCR ile incelenmesi

Bu çalışmanın amacı Tannerella forsythia’nın primer kök kanal enfeksiyonlarındaki prevalansını ve bu bakterinin değişik formlardaki periradiküler hastalıklar ile ilişkisini Polimeraz Zincir Reaksiyonu PCR yöntemi ile incelemektir. Örnekler, akut ve kronik formlardaki periradiküler hastalıklar ve abselerden elde edildi. Filtreli kolon DNA izolasyon kiti kullanılarak DNA ekstraksiyonu yapıldı ve 16S rRNA gen bazlı PCR yürütüldü. Verilerin istatistiksel analizinde Ki - kare testi kullanıldı. Akut apikal periodontitisli 26 vakanın 13’ünde %50 , akut periradiküler abseli 7 vakanın 3’ünde %42,8 ve kronik asemptomatik periradiküler lezyonlu 25 vakanın 2’sinde %8 olmak üzere toplam 58 vakanın 18’inde %31,3 Tannerella forsythia DNA’sı saptandı. Bakteri varlığı ile akut apikal periodontitis grubu arasındaki ilişki istatistiksel olarak önemli bulundu P=0.002 . Tannerella forsythia periradiküler hasarın akut semptomları ile ilişkili potensiyel bir endodontik patojen olarak düşünülmelidir.

Anahtar Kelimeler:

Enfeksiyon, PCR, kök kanalı, rRNA, Tannerella forsythia

Prevalance of Tannerella Forsythia in Primary Root Canal Infections by 16S rRNA Based PCR

The purpose of this study was to assess the prevalence of Tannerella forsythia in the primary infections of root canals and its association with different forms of periradicular diseases using Polymerase Chain Reaction PCR . Samples were collected from acute and chronic forms of periradicular diseases and abscesses. DNA extraction was performed using spin column DNA isolation kit and 16S rRNA gene based PCR analysis was performed. Chi-squared test was used for data analysis. Tannerella forsythia DNA was detected in 18 31,3% of the total 58 samples, in 13 of 26 cases 50% with acute apical periodontitis, three of seven cases 42,8% with acute periradicular abscesses, and 2 of 25 cases 8% with chronic asymptomatic periradicular lesions. It was found to be significantly related with acute apical periodontitis group p=0.002 . T. forsythia must be considered as a potential endodontic pathogen associated with acute symptoms of periradicular disease.

Keywords:

Infection, PCR, root canal, rRNA, Tannerella forsythia,

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Rocas IN, Siqueira JF Jr, Santos KR, Coelho AM. ‘Red complex’ (Bacteroides forsythus, Porphyromonas denticola) in endodontic infections: a molecular approach. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2001; 91: 468-71. and Treponema
Fouad AF, Barry J, Caimano M, Clawson M, Zhu Q, Carver R et al. PCR-based identification of bacteria associated with endodontic infections. J Clin Microbiol 2002; 40: 3223-31.
Tanner ACR, Listgarten MA, Ebersole JL, Strzempko MN. Bacteroides forsythus sp. nov., a slow-growing fusiform Bacteroides sp. from the human oral cavity. Int J Syst Bacteriol 1986; 36: 213-21.
Siqueira JF Jr, Rocas IN. PCR methodology as a valuable tool for identification of endodontic pathogens. J Dent 2003; 31: 333-9.
Foschi F, Cavrini F, Montebugnoli L, Stashenko P, Sambri V, Prati C. Detection of bacteria in endodontic samples by polymerase chain reaction assays and association with defined clinical signs in Italian patients. Oral Microbiol Immunol 2005; 20: 289-95.
Jung IY, Choi BK, Kum KY, Roh BD, Lee SJ, Lee CY, Park DS. Molecular epidemiology and association of putative pathogens in root canal infection. J Endod 2000; 26: 599-604.
Siqueira JF Jr, Rocas IN, Alves FR, Santos KR. Selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. J Endod 2004; 30: 638-43.
Siqueira JF Jr, Rocas IN. Bacteroides forsythus in primary endodontic infections as detected by nested PCR. J Endod 2003; 29: 390-3.
Siqueira JF Jr, Rocas IN, Moraes SR, Santos KR. Direct amplification of rRNA gene sequences for identification of selected oral pathogens in root canal infections. Int Endod J 2002; 35: 345-51.
Saito D, Coutinho LL, Borges Saito CP, Tsai SM, Höfling JF, Gonçalves RB. Real-time polymerase Porphyromonas gingivalis and Tannerella forsythia in primary endodontic infections. J Endod 2009; 35: 1518-24. quantification of
Lacević A, Pojskić LK, Lojo NK, Ramić J, Bajrović K. Tannerella forsythia detected in infected 11 root canals using nested PCR. Am J Dent 2009; 22: 211-4.
Lu JJ, Perng CL, Lee SY, Wan CC. Use of PCR with Universal Primers and Restriction Endonuclease Identification of Common Bacterial Pathogens in Cerebrospinal Fluid. J Clin Microbiol 2000; 38: 2076-80. for Detection and
Niu SQ, Fukushima J, Jiang Y, Ishikawa Y, Ueda T, Matsumoto S. Analysis of Bacterial Community Structure in the Natural Circulation System Wastewater Bioreactor by Using a 16S rRNA Gene Clone Library. Microbiol Immunol 2006; 50: 937-50. 14. Wade W. Unculturable bacteria-the uncharacterized organisms that cause oral infections. J R Soc Med 2002; 95: 81-3.
Wyss C. Dependence of proliferation of on Bacteroides acetylmuramic acid. Infect Immun 1989; 57: 1757-9. exogenous N
Baumgartner JC, Siqueira JF Jr, Xia T, Rocas IN. Geographical differences in bacteria detected in endodontic infections using polymerase chain reaction. J Endod 2004; 30: 141-4.
Siqueira JF, Jung IY, Rocas IN, Lee CY. Differences in prevalence of selected bacterial spe- cies in primary endodontic infections from two dis- tinct geographic locations. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2005; 99: 641-7.
Huang Y, Umeda M, Takeuchi Y, Ishizuka M, Yano-Higuchi K, Ishikawa I. Distribution of Bacteroides forsythus genotypes in a Japanese periodontitis population. Oral Microbiol Immunol 2003; 18: 208-14.
Hudspeth MK, Hunt Gerardo S, Maiden MF, Citron DM, Goldstein EJ. Characterization of Bacteroides forsythus strains from cat and dog bite wounds in humans and comparison with monkey and human oral strains. J Clin Microbiol 1999; 37: 2003-6.
Takemoto Characterization of Bacteroides forsythus isolates. J Clin Microbiol 1997; 35: 1378-81. Dahlen G.