Potency of rhodococcus equi culture filtrate supernatant proteins antigen for skin test in the diagnosis of rhodococcus equi in foals

Amaç: Araştırma Rhodococcus equi protein antijenlerinin deri testinin geliştirilmesi için dizayn edildi.Gereç ve Yöntem: R. equi ile enfekte 10 adet Arap atı tayına 0.1 mL (12.5 $mu$/mL) Rhodococcus antiji ve 10 adet sağlıklı kontrol grubuna 0.1 mL PBS bacak bölgesine deri içi yolla enjekte edildi. Deri kalınlıkları uygulama öncesi ve sonrası 24, 48 ve 72. saatlerde ölçüldü. Deri biyopsisi uygulama sonrasında 72. saatte yapıldı. Bulgular: Deri reaksiyonları uygulama sonrası 24. saatte hafif, 72. saatte ise yoğun gözlendi. Yapılan biyopsilerde makrofaj, nötrofil ve eozinofil gibi yangı hücreleri belirlendi. Kontrol grubu taylarda uygulama sonrası gözle görülen bir reaksiyon gözlenmedi. Öneri: Rhodococcus-kültür antijeninin, R. equi'ye mâruz kalan veya enfekte olan taylarda hastalığın tanısında diagnos-tik öneminin olabileceği belirlendi. Mevcut araştırmanın sonuçları dikkate alındığında, R. equi ile enfekte haralarda deri testinin hastalığın tanısında umut vaat etmektedir.

Taylarda rhodococcus equi tanısı deri testinde rhodococcus equi kültür filtrat supernatant protein antijeninin değerlendirilmesi

Aim: This study was designed to developing skin test proteins antigen prepared from Rhodococcus equi isolates.Materials and Methods: Ten Arabian foals experimentally infected with R. equi were injected intradermaly with 0.1 mL (12.5 ($mu$/mL) Rhodococcus antigen and 0.1 mL PBS as control each treatment at one side of neck region, while 10 healthy foals had the same treatment and used as control group. Skin thickness was measured prior and at 24, 48 and 72 hours post injection by skin caliber. Skin biopsy was taken from the site of reaction at 72 hours post injection.Results: All infected foals had slightly skin reaction at 24 hours, and maximum skin reaction was determined at 72 hours post inoculation. Inflammatory cells including macrophages, neutrophils and eosinophils were present in the sections prepared from biopsy obtained from positive skin reaction. All control foals revealed no visible reaction in the site of injection. Conclusion: Partially purified Rhodococcus-culture antigen is potential diagnostic antigens able to detect foals previously infected or exposed to R. equi infection. Our data provide a promising basis for the future development of screening diagnostic skin tests for R. equi infection in infected farms.

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Black CA, 1999. Delayed type hypersensitivity: Current theories with an historic perspective. Dermatology Online Journal, 5, 7.
Dannenberg JAM, 1991. Delayed-type hypersensitivity and cell-mediated immunity in the pathogenesis of tuberculosis. Immunol Today, 12, 228-33.
Ellenberger MA, Kaeberle ML, Roth JA, 1984. Equine cell-mediated immune response to Rhodococcus (Coryne-bacterium) equi. Am J Vet Res, 45, 2424
Gansert JL, Kiessler V, Engele M, Wittke F, Rollinghoff M, Krensky AM, Porcelli SA, Modlin RL, Stenger S, 2003. Human NKT cells express granulysin and exhibit an-timycobacterial activity. J Immunol, 170, 3154-3161.
Giguere S, Prescott JF, 1997. Clinical manifestations, diagnosis, treatment, and prevention of Rhodococcus equi infections in foals. Vet Microbiol, 56, 313-334.
Hines MT, Paasch KM, Alperin DC, Palmer GH, Westhoff NC, Hines SA, 2001. Immunity to Rhodococcus equi: antigen-specific recall responses in the lungs of adult horses. Vet Immunol Immunopathol, 79,101-114.
Hines SA, Stone DM, Hines MT, Alperin DC, Knowles DP, Norton LK, Hamilton MJ, Davis WC, McGuire TC, 2003. Clearance of virulent but not avirulent Rhodococcus equi from the lungs of adult horses is associated within tracytoplasmic gammainterferon production by CD4 and CD8 T lymphocytes. Clin Diagn Lab Immunol, 10, 208-215.
Kanaly ST, Hines SA, Palmer GH, 1995. Cytokine modulation alters pulmonary clearance of Rhodococcus equi and development of granulomatous pneumonia. Infect Im-mun, 63, 3037-3041.
Kanaly ST, Hines SA, Palmer GH 1993. Failure of pulmonary clearance of Rhodococcus equi infection in CD4+ T-lymphocyte-deficient transgenic mice. Infect Immun, 61, 4929-4932.
Macias J, Pineda JA, Borderas F, Gallardo FA, Delgado J, Leal M, Sanchez-Quijano A, Lissen E, 1997. Rhodococcus or mycobacterium? An example of misdiagnosis in HIV infection. AIDS, 11,253-254.
Nordmann P, Ronco E, Nauciel C, 1992. Role of T-lymphocyte subsets in Rhodococcus equi- infection. Infect Immun, 60, 2748-2752.
Giguere S, Hernandez J, Gaskin J, Prescott JF, Takai S, Miller C, 2003. Performance of five serological assays for diagnosis of Rhodococcus equi pneumonia in foals. Clin Diagn Lab Immunol, 10, 241-245.
Wilson MM, 1955. A study of Corynebacterium equi infection in a study of Thoroughbred horses in Victoria. Aust Vet J, 31,175-181.