Muhammet Lütfi SELÇUK, Durmuş BOLAT, Sadullah BAHAR, Sadettin TIPIRDAMAZ

Morphometric investigations of fresh and fixed rabbit kidney

Amaç: Bu çalışmada formaldehit tespitinin böbrek morfo-metrisine etkisi ile böbreği oluşturan yapıların hacim ölçümlerinin ve ilgili yapıların böbrek içerisindeki hacimsel oranlarının Cavalieri prensibi kullanarak değerlendirilmesi amaçlandı.Gereç ve Yöntem: Çalışmada 9 adet sağlıklı erkek Yeni Zelanda beyaz tavşanına ait böbrekler kullanıldı. Taze böbreklere ait ağırlık verileri elde edildikten sonra craniocaudal uzunluk, dorsoventral ve mediolateral çap ölçümleri dijital kumpas yardımı ile yapıldı. Her bir böbreğe ait hacim ölçümleri Arşimed prensibi ile gerçekleştirildi. Bu işlemlerden sonra taze böbrekler %10 formol içeren kaplarda 15 gün süre ile tespit edildi. Tespit edilen böbrekler üzerinde taze böbreklerde yapılan ölçüm işlemleri tekrarlandı ve böbreği oluşturan yapıların (korteks, medulla ve pelvis re-nalis) hacimleri Cavalieri prensibi ile hesaplandı.Bulgular: Taze (15.8±0.26 mm) ve tespit edilmiş (14.8±0.32 mm) sağ böbreklere ait dorsoventral çap değerleri arasında istatistiki olarak önemli fark tespit edildi (p

Taze ve tespit edilmiş tavşan böbreği üzerinde morfometrik araştırmalar.

Aim: The aim of this study was to investigate the effect of formalin fixation on the morphometry of kidneys, and to assess the volume and fractions of its constituents using the Cavalieri principle. Materials and Methods: In this study, kidneys of nine healthy male White New Zealand rabbits were used. After weighing the kidneys, craniocaudal length, and mediolateral and dorsoventral diameters of fresh kidneys were measured with digital calipers, and the volume of each kidney was estimated using Archimedes' principle. The fresh kidneys were kept in a container of 10% formalin solution for 15 days. The Cavalieri principle was utilized for the determination of volume and the volume fraction of renal constituents. Results: The differences in the dorsoventral diameter of fresh (15.8±0.26 mm) and fixed (14.8±0.32 mm) right kidneys were found to be statistically significant (p

PDF

___

Akdoğan I, Kiroglu Y, Onur S, Karabulut N, 2010. The volume fraction of brain ventricles to total brain volume: a computed tomography stereological study. Folia Morphol, 69,193-200.
Akosman MS, Özdemir V, 2010. Capability of the Cavalieri method for volume estimation of the dog testis. Eurasian J Vet Sci, 26, 63-67.
Altunkaynak ME, Özbek E, Altunkaynak BZ, Can I, Unal D, Unal B, 2008. The effects of high fat diet on the renal structure and morphometric parametric of kidneys in rats. J Anat, 212,845-852.
Bailey SA, Zidell RH, Perry RW, 2004. Relationships between organ weight and body/brain weight in the rat: What is the best analytical endpoint? Toxicol Pathol, 32, 448-466.
Bas 0, Acer N, Mas N, Karabekir HS, Kusbeci OY, Sahin B, 2009. Stereological evaluation of the volume and volume fraction of intracranial structures in magnetic resonance images of patients with Alzheimer's disease. Ann Anat, 191,186-195.
Christiansen T, Rasch R, Stodkilde-J0rgensen H, Flyvbjerg A, 1997. Relationship between MRI and morphometric kidney measurements in diabetic and non-diabetic rats. Kidney Int, 51,50-56.
Cibulskyte D, Samsoe Engberg A, Hanefelt Kristensen D, El-lingsen AR, Pedersen M, Hoerlyck A, Flyvbjerg A, Mar-cussen N, Hansen HE, Madsen M, 2007. Renal effects of long-term ciclosporin A treatment in a large animal model. Nephron Exp Nephrol, 105, e91-e97.
Cotter D, Miszkiel K, Al-Sarraj S, Wilkinson I, Paley M, Harrison M, Hall-Craggs M, Everall 1,1999. The assessment of postmortem brain volume; a comparison of stereological and planimetric methodologies. Neuroradiology, 41, 493-496.
Coulam CH, Bouley DM, Sommer FG, 2002. Measurement of renal volumes with contrast enhanced MRI. J Magn Re-son Im, 15,174-179.
Gundersen HJ, Jensen EB, Kieu K, Nielsen J, 1999. The efficiency of systematic sampling in stereology-reconsid-ered. J Microsc, 193,199-211.
Hall V, Macgregor W, 1937. Relation of kidney weight to body weight in the cat. Anat Rec, 69, 319-331.
Heuer R, Sommer G, Shortliffe LD, 2003. Evaluation of renal growth by magnetic resonance imaging and computerized tomography volumes. J Urology, 170,1659-1663.
Karstoft K, L0drup AB, Dissing TH, S0rensen TS, Nyengaard JR, Pedersen M, 2007. Different strategies for MRI measurements of renal cortical volume. J Magn Reson Im, 26,1564-1571.
Lipman NS, Marini RP, Erdman SE, 1990. A comparison of ketamine xylazine and ketamine xylazine acepromazine anesthesia in the rabbit. Lab Anim Sci, 40, 395-398.
Malas A, Sulak O, Ungor B, Çetin E, Albay S, 2002. Determination of the volume of renal morphological structure by stereological method. SDU Tp Fak Derg, 9,1-5.
Mayhew TM, Gundersen HJ, 1996. If you assume, you can make an ass out of u and me': a decade of the disector for stereological counting of particles in 3D space. J Anat, 188 (Pt 1), 1-15.
Michael B, Yano B, Sellers RS, Perry R, Morton D, Roome N, Johnson JK, Schafer K, 2007. Evaluation of organ weights for rodent and non-rodent toxicity studies: a review of regulatory guidelines and a survey of current practices. Toxicol Pathol, 35, 742-750.
Mounier-Vehier C, Lions C, Devos P, Jaboureck 0, Willoteaux S, Carre A, Beregi JP, 2002. Cortical thickness: an early morphological marker of atherosclerotic renal disease. Kidney Int, 61, 591-598.
Mouton PR, 2002. Principles and practices of unbiased ster-eology, 211 pages, John Hopkins University Press, Baltimore, Maryland.
Nawaratne S, Brien JE, Seeman E, Fabiny R, Zalcberg J, Coso-lo W, Angus P, Morgan DJ, 1998. Relationships among liver and kidney volumes, lean body mass and drug clearance. Br J Clin Pharmacol, 46,447-452.
Padigala KK, Hartle JE, Kirchner HL, Schultz MF, 2009. Renal Cortical Thickness as a Predictor of Renal Function and Blood Pressure Status Post Renal Artery Stenting Angi-ology, 60, 719-724.
Pazvant G, Şahin B, Kahvecioglu KO, Gunes H, ince NG, Ba-cinoglu D, 2009. The volume fraction method for the evaluation of kidney: A stereological study. Ankara Univ Vet Fak Derg, 56, 233-239.
Prasad PV, 2006. Functional MRI of the kidney: tools for translational studies of pathophysiology of renal disease. Am J Physiol-Renal, 290, F958-F974.
Prince MR, Schoenberg SO, Ward JS, Londy FJ, Wakefield TW, Stanley JC, 1997. Hemodynamically significant atherosclerotic renal artery stenosis: MR angiographic features. Radiology, 205,128-136.
Slomianka L, West MJ, 2005. Estimators of the precision of stereological estimates: an example based on the CA1 pyramidal cell layer of rats. Neuroscience, 136,757-767.
Stowell RE, 1941. Effect on tissue volume of various methods of fixation, dehydration, and embedding. Biotech Histochem, 16, 67-83.
Taylor FB, Drury DR, Addis T, 1923. The regulation of renal activity. VIII. The relation between the rate of urea excretion and the size of the kidneys. Am J Physiol, 65, 55-61.
Turgut M, Turkkani Tunc A, Aslan H, Yazici A, Kaplan S, 2007. Effect of pinealectomy on the morphology of the chick cervical spinal cord: A stereological and histopathologi-cal study. Brain Res, 1129,166-173.