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Koyun fötuslarında Border Disease Virus varlığının immunperoksidaz ile saptanması

Amaç: Bu çalışma Konya'da koyun fötuslarında pestiviral antijenlerin araştırılması amacı ile yapıldı.Gereç ve Yöntem: Araştırmada 34 adet koyun fötusundan elde edilen toplam 170 doku örneği (karaciğer, akciğer, lenf, dalak ve beyin) Border Disease Virus (BDV) antijen varlığı yönünden Madin-Darby Bovine Kidney devamlı hücre kültürlerinde immunperoksidaz (IP) testi ile incelendi.Bulgular: Fötusların %8.82'sinde pestiviral antijen varlığı belirlendi. 3 adet fötusa ait 8 doku örneği (3 karaciğer, 3 akciğer ve 2 beyin) pozitif tespit edildi. Aynı fötuslara ait lenf ve dalak örneklerinin hiçbirisinde pozitiflik belirlenmedi.Öneri: Konya'da BDV ile mücadele edilmesi gerekliliği ifade edilebilir.

Detection of Border Disease Virus in sheep fetuses by immunoperoxidase

Aim: The purpose of the present study was to investigate pestiviral antigen in sheep fetuses in Konya.Materials and Methods: Totally 170 samples (liver, lung, lymph, spleen, and brain) from 34 sheep fetuses were investigated to determine the presence of Border Disease Virus (BDV) with immunoperoxidase (IP) in Madin-Darby Bovine Kidney permanent cell culture.Results: Presence of pestiviral antigen was detected by IP in 8.82% of fetuses. 8 tissue (3 livers, 3 lungs, and 2 brains) samples of 3 fetuses were detected as positive. Lymph and spleen samples of the same fetus were not determined in any positivity.Conclusion: It can be expressed that control programme of BDV is needed in Konya.

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