Aytaç AKÇAY, Aydın ALAN, İsmail Hakkı NUR, Atilla YOLDAŞ

A Scanning Electron Microscopic studies on the podocytes of the kidneys of rat (Rattus norvegicus) and blind mole rat (Spalax leucodon)

Amaç: Kör fareler yer altında kendi kazdıkları tünellerde oksijen oranı oldukça düşük ortamlarda yaşayabilmektedirler. Çoğu kemiricilerden farklı olarak, kör fareler bu koşullarda metabolik faaliyetlerini yüksek oranda yerine getirebilmektedirler. Mevcut çalışmada kör fare ile ratların glomerulusların, fonksiyonlarını ve normal yapılarını sürdürebilmeleri için önemli olan podositlerinin incelemesi amaçlandı.Gereç ve Yöntem: Çalışmada altı adet erkek kör fare ve altı adet erkek rat kullanıldı. Rat ve kör farelerin podositleri morfolojik özelikleri Scaning Electron Mikroskobu (SEM) kullanılarak incelendi.Bulgular: Kör farelerin böbrek uzunluğu, genişliği ve kalınlığı 15.6±0.25, 9.60±0.24, 7.80±0.37 cm iken, bu ölçüler ratda sırası ile 16.00±0.89, 9.40±0.74, 6.20±1.35 cm olarak belirlendi. Bowman kapsulu çapının ve Bowman aralığının ratlarda, kör farelerden daha büyük olduğu tespit edildi. Filtrasyon aralığı kör farelerde 0.049±0.003 μm iken, ratlarda 0.073±0.004 μm olarak ölçüldü. Podosit pedicel'lerinin şekillerinin birbirine benzer ve iğne şeklinde oldukları, uzunluklarının 0.88 ile 1.02 μm arasında değiştikleri ve her iki türde de hücrelerin kenar uzunlukları boyunca birbirine kenetlendikleri tespit edildi.Öneri: Kör farelerin podositlerinin morfolojik olarak ratlarla aynı olduğu tespit edildi. Her iki türde glomerulusun çapları arasında istatistiksel olarak bir farklılık olmadığı belirlendi (P>0.05). Bunun yanında böbreklerin uzunluk, kalınlık ve genişlikleri arasında da istatistiksel bir farklılık olmadığı tespit edildi.

Kör fare (Spalax leucodon) ve rat (Rattus norvegicus) böbreklerinde podositler üzerinde elektron mikroskobik bir çalışma

Aim: Blind mole rats can live in tunnels which are goosed by them underground, in environments that have quite low-oxygen ratio. Contrary most rodents, they can carry out high levels of metabolic rate under these conditions. The present study aimed out to compare the blind mole rats and rats's renal podocyte, an important cell for maintaining the normal structure and function of the glomerulus.Materials and Methods: Blind mole rat (Male) (n=6) and rats (Male) (n=6) were used in this study. The morphologic features of the renal podocytes of blind mole rat and rat kidneys were examined by using scanning electron microscopic (SEM).Results: While the length, width and thickness of blind mole rat kidney were 15.6±0.25, 9.60±0.24 and 7.80±0.37 cm, in rats were 16.00±0.89, 9.40±0.74, 6.20±1.35 cm, respectively. When compared Bowman's capsule diameters and Bowman slits of blind mole rat, the rat was measured to be larger. The mean length of Pedicel spacing (Filtration slits) of blind mole rat and rats were found to be 0.049±0.003 μm and 0.073±0.004 μm, respectively. The podocyte pedicels were uniform needle-like shapes, and its length was ranging from 0.88 to 1.02 μm. They were in contact with each other intertwined processes in both species investigated.Conclusion: The podocyte of blind mole rat is morphologically similar to rat's. In both species, between to diameter of the glomerulus were not statistically significant differences (P>0.05). Furthermore, there were not statistically significant differences in the length, the width and the thickness of both species' kidneys (P>0.05).

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