Hatice ESER, Burak DİK, Enver YAZAR, Ayşe ER, Orhan ÇORUM, Emre BAHÇİVAN

Koçlara deksametazon uygulamasının kan oksidatif durum ve prostaglandin F2α metaboliti düzeyine etkisi

Amaç: Bu araştırmanın öncelikli amacı sağlıklı koçlara deksametazon uygulamasının serum tiyobarbitürik asit reaktif ürünleri (TBARS) ve plazma 13,14-dihidro-15-keto-prostaglandin F2? (PGM) düzeyine etkisini belirlemektir. Ayrıca deksametazonun hemogram ve serum biyokimyasal parametrelere etkisini değerlendirmektir. Gereç ve Yöntem: Araştırmada 10 adet koça derialtı yolla tek sefer 0.5 mg/kg dozda deksametazon uygulandı. Hayvanlardan uygulama öncesi (0. saat, Kontrol grup) ve sonrası 0.25, 0.5, 1, 2, 4, 8, 12 ve 24. saatlerde kan örnekleri alındı. Serum TBARS ve plazma PGM düzeyleri ELISA okuyucusunda belirlendi. Hemogram değerleri kan hücresi sayım cihazında ölçülürken, biyokimyasal parametreler otoanalizörde ölçüldü.Bulgular: Deksametazonun serum TBARS düzeyinde istatistiki olmayan dalgalanmalara neden olduğu belirlenirken, plazma PGM düzeyine etkisinin olmadığı tespit edildi. Akyuvar ve serum glikoz düzeylerinde ise yükselmelere (P

Effect of dexamethasone treatment on blood oxidative status and prostaglandin F2α metabolite levels in ram.

Aim: The first aim of this research was to determine the effects of dexamethasone on serum thiobarbituric acid reactive substances (TBARS) and plasma 13,14-dihydro-15-keto-prostaglandin-F2α (PGM) levels in healthy rams. In addition, effects of dexamethasone on the hemogram and biochemical parameters were evaluated. Materials and Methods: Single dose dexamethasone (0.5 mg/kg, SC) was administered to 10 rams. Blood samples were collected before (0. hour, Control group) and after drug treatment at 0.25, 0.5, 1, 2, 4, 8, 12 and 24 hours. Serum TBARS and plasma PGM levels were measured with ELISA reader. Hemogram values were determined with hemacell counter, whereas biochemical values were measured with auto-analyzer. Results: Dexamethasone caused statistically not significant fluctuations in the serum TBARS levels while it did no effect plasma PGM levels. Dexamethasone increased (P

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