Investigation of tetracycline residues in fish caught from surrounding fish farms in Muğla district

Amaç: Su ürünlerindeki ilaç ve metabolitlerinin kalıntı analizleri Ulusal Kalıntı İzleme Planı kapsamında düzenli olarak yapılmaktadır. Fakat Türkiye'de bu kalıntıların sucul ortama etkilerine yönelik araştırmalar sınırlıdır. Bu çalışmada, Muğla bölgesindeki balık çiftliklerinin etrafından avlanan balıklarda tetrasiklin grubu antibiyotiklerin (oksitetrasiklin, tetrasiklin, klortetrasiklin, doksisiklin) kalıntılarının belirlenmesi amaçlandı. Gereç ve Yöntem: Analiz için 70 ayrı çiftlik etrafından toplanan 70 adet balık örneği kullanıldı. Balıklarda tetrasiklin kalıntıları LC MS/MS yöntemi ile analiz edildi. Seçicilik, doğ- rusallık, geri kazanım, duyarlılık ve kesinlik ölçütlerinde metot validasyonu yapıldı. Bulgular: Analizin geri kazanımları oksitetrasiklin için %100.5, tetrasiklin için %101.3, klortetrasiklin için %99 ve doksisilin için %100.5 olarak belirlendi. Analizlerin sonucunda balık örneklerinin hiçbirinde tetrasiklin grubu antibiyotikler tespit edilemedi. Öneri: Yapılan analizlerde balık örneklerinde hesaplanabilir limitlerde tetrasiklin kalıntısı bulunmaması çevre ve halk sağlığı açısından memnuniyet verici olarak kabul edilebilir.

Turk E, Oguz H. Muğla bölgesindeki balık çiftlikleri çevresinden avlanan balıklarda tetrasiklin aranması

Aim: Residue analyses of drugs and metabolites in the aquacultureare regularly conducted under the National ResidueMonitoring Plan in Turkey. However, the research on the impactof these residues on the aquatic environment is limited.In this study, it was aimed to analyze the residues of tetracyclinegroup antibiotics in fishes caught around fish farms inthe Muğla district.Materials and Methods: Seventy fish samples collectedfrom the vicinity of 70 different fish farms were used foranalysis. Tetracycline residues in the fish were analyzed withthe LC MS/MS method. Validation was performed with thecriteria of specificity, linearity, recovery, precision and sensitivity. Results: Recovery values were determined as follows: Oxytetracycline100.5%, tetracycline 101.3%, chlortetracycline99%, and doxycycline 100.5%. At the end of the analyses, notetracycline antibiotic residues were found.Conclusion: It can be expressed that no tetracycline residueswere detected in the samples at the detectable limits is a satisfactoryresult in terms of public health and environment.

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