Şule SARIÇAM, K. Yaprak KANTOĞLU, Ş. Şebnem ELLİALTIOĞLU

Determination of Effective Mutagen Dose for Lettuce (Lactuca sativa var. longifolia cv. Cervantes) Seeds

In plant breeding strategies induced mutagenesis has become an effective way of supplementing the existing germplasm and improving new varieties. Mutation can be induced by chemical and physical mutagens or by combination. The commercial importance and production of lettuce, which is the most popular of the local salad crops, is increasing in Turkey. Lettuce is an important vegetable commodity and in demand by the local markets throughout the year. This study was carried out to get database for mutation breeding studies on lettuce cv. Cervantes. For this aim, 0, 50, 100, 200, 300, 400, 500 and 600 Gray (Gy) doses of Co60 (gamma-rays) were treated on lettuce seeds as a physical mutagen. 30 seeds were used for each dose. Thirty days after treatment, germination and shoot developing of cloves were determined. The Effective Mutagen Dose (EMD50) calculated by linear regression analyses. According to results, 372.66 Gy dose was found as EMD50.

Keywords:

Lettuce, mutation breeding, Co60 gamma ray, EMD50, Lactuca sativa,

PDF

___

Anonymus 1977. “Manual on Mutation Breeding”. International Atomic Energy Agency, Technical Report Series No:119, Vienna. 290p.
Anonymus 2016. Turkish Statistical Institute, https://biruni.tuik.gov.tr/bitkiselapp/bitkisel.zul.
Anonymus, 1977. “Manual on Mutation Breeding”. International Atomic Energy Agency, Technical Report Series No:119, Vienna. 290p,
Dayton Wilde H., Chen Y., Jiang P. and Bhattacharya A. 2012. “Targeted mutation breeding of horticultural plants”, Emir. J. Food Agric. 24 (1): 31-41.
De Vries I. M.1997. “Origin and domestication of Lactuca sativa L. genetic resources and crop evolution”, 44, 165–174.
Franco C. H., Santos H.M., Silva L. P., Arthur V. and Silva R. G. M. 2015. “Mutagenic Potential of Lettuce Grown from Irradiated Seeds", Scientia Horticulturae 182, 27–30.
Grube R. C., Brennan E. B. and Ryder E. J. 2003. “Characterization and genetic analysis of a lettuce (Lactuca sativa L.) mutant, weary, that exhibits reduced gravitropic response in hypocotyls and inflorescence stems”, Journal of Experimental Botany, 54: 385, 1259-1268.
Masuda M., Agong S., Tanaka A., Shikazono N. and Hase Y. 2004. “Mutation spectrum of tomato seed induced by radiation with helium ion beams and coal”, Acta Hort. 637, 257-262.
Mou, B., 2011. “Mutations in Lettuce Improvement”, International Journal of Plant Genomics, 2011(3): 723518.
Mou B. 2008. Lettuce. Handbook of Plant Breeding, Vegetables I, Asteraceae, Brassicaceae, Chenopodicaceae and Cucurbitaceae, Page: 85-126.
Ohki S. and Hatashita M. 2012. “Mutation breeding by ion beam in lettuce (Lactuca sativa L.) using an in vitro regeneration”, Research and Development Department Proc, 7th ISHS on In Vitro Culture and Horticultural Breeding (Ed.: D. Geelen) Acta Hort. 961, 285-290.
Okubara P. A., Anderson P. A., Ochoa O. E. and Michelmore R. W. 1994. “Mutants of downy mildew resistance in Lactuca sativa (lettuce)”, Genetics, 137: 3, 867–874.
Sagel Z., Peskircioglu H., Tutluer I., Uslu N., Senay A., Taner K.Y., Kunter B., Sekerci S. and Yalcin S. 2002. “Bitki Islahında Mutasyon ve Doku Kültürü Teknikleri”, III. Ulusal Mutasyon Kursu Kurs Notları, TAEK, ANTHAM Nükleer Tarım Radyobiyoloji Bölümü, Ankara.
Waycott W., Fort S. B. T and Ryder E. J. 1995, “Inheritance of dwarﬁng genes in Lactuca sativa L.”, Journal of Heredity, 86:1, 39–44.