Türkiye’de Kronik İshalli Kedilerde Tritrichomonas foetus’ün Araştırılması ve Risk Faktörlerinin Belirlenmesi
Bu çalışma ile Türkiye’de ilk kez evcil kedilerin kalın bağırsak ishallerinde Tritrichomonas foetus’ün etiyolojik ajan olarak rol oynayıp-oynamadığının moleküler teknikler ile araştırılıp enfeksiyonun risk faktörlerinin belirlenmesi amaçlanmıştır. Ondokuz Mayıs Üniversitesi Veteriner Fakültesi İç Hastalıkları Anabilim Dalı Kliniklerine kronik ishal şikâyeti ile getirilen farklı ırk, yaş, cinsiyet, beslenme ve yaşama koşullarına sahip 50 kedi ile Samsun Büyükşehir Belediyesi Güçten Düşmüş Hayvan Bakım Evi’nden 50 adet kronik ishalli kedilere ait dışkı örnekleri bireysel olarak toplanmıştır. Toplanan 100 dışkı örneğinden genomik DNA ekstraksiyonu yapılmış ve sonrasında parazitin varlığının araştırılmasında cysteine protease 2 (CP-2) ve internal transcribed spacer (ITS) geninin invitro koşullarda amplifikasyonu için PCR metodu kullanılmıştır. Araştırma sonucunda kronik ishalli kedilere ait olan 100 dışkı örneğinde T. foetus pozitifliği saptanmamıştır. Bu araştırma ile ülkemizdeki kronik ishalli kedilerde ilk kez moleküler olarak T. foetus’un varlığı araştırılmıştır.
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