Türkiye’de İshalli Buzağılardan Bovine Astrovirus’un İlk Teşhisi ve Filogenetik Analizi

Orta Anadolu’da yer alan üç ilden (Sivas, Malatya ve Elazığ), 2014-16 yılları arasında toplam 127 dışkı örneği toplandı. Bovine astrovirus’un Nsp1ab geninin 432 bp kısmi bölgesi çoğaltıldı, dizi analizi yapıldı ve GenBank’a yüklendi. Sekans hizalaması ve filogenetik analizler yapılarak moleküler karakterizasyonu gerçekleştirildi. RT-PCR çalışmasının sonucuna göre örneklerin %3,15’i (4/127) pozitif olarak tespit edildi. Kısmi Nsp1ab gen (ORF1a) bölgesinin neighbour-joinning yöntemiyle filogeni ağacı oluşturuldu. Buna göre izolatlarımızın hiçbiri sinirsel belirti gösteren sığırlardan izole edilen Neuro1 hattında (hat 3) yer almazken BAstV-HT1-TUR suşumuz hat 1a altında ve diğer suşlarımız ise hat 2 altında konumlandı. Analiz edilen sekansların benzerliği %51,1’den %100’e varan oranlarda gerçekleşirken yeni suşların kendi arasındaki benzerlik oranının %75,8’den %100’e vardığı ortaya konuldu. Sonuç olarak, Bovine astrovirusun Türkiye’de ilk teşhisi ve filogenetik analizi rapor edilmiştir.

The First Detection and Phylogenetic Analysis of Bovine Astrovirus from Diarrheic Calves in Turkey

A total of 127 stool samples (rectal swab) from diarrheic calves, up to one mouth of age, were collected during one year period (2014-16) from three cities (Sivas, Malatya and Elazığ) located central Turkey. 432 bp partial sequence of Nsp1ab gene were amplified. The PCR amplicons were purified and sequenced and deposited to GenBank. Sequence alignment and phylogenetic analysis being based on partial nucleotide sequences of Nsp1ab gene was constructed. As a result of RT-PCR study, we found that the 3.15% of fecal samples (4/127) were positive for diarrhea calves from Turkey. The neighbor-joinning tree of partial sequence of Nsp1ab gene (ORF1a) indicated that strains were substituted under three distinct lineage. None of our strains belonged to the Neuro1 lineage (lineage 3) of Bovine astrovirus which was isolated from bovine with neurological symptoms. On the other hand, while BAstVHT1-TUR strain was substituted under lineage 1a, the other novel starts were lineage 2. While the identity of analyzed sequences varies from 51.1 to 100%, novel strains were calculated between 75.8 to 100 % each other. As a result, we report the first detection and phylogenetic analysis of Bovine astrovirus from Turkey.

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